卫矛醇对C6胶质瘤细胞体外抗癌活性及机制探讨

目的:研究卫矛醇对C6胶质瘤细胞株的体外增殖及凋亡的影响。方法：将C6大鼠胶质瘤细胞随机分为对照组、卫矛醇干预组、卫矛醇+雷帕霉素组、卫矛醇+3-MA组,各组予不同药物孵育,采用MTT法检测卫矛醇对C6细胞的体外增殖抑制率,筛选出抑制率相对较高且生长状态良好的细胞;应用Hoechest 33342染色透射电镜观察细胞凋亡亚显微结构的改变,采用免疫荧光和Western Blot技术检测与凋亡和自噬相关蛋白的表达水平。结果:卫矛醇对C6胶质瘤细胞增殖有明显的抑制作用,并呈现出良好的浓度-效应相关性,与对照组比较,随着浓度的增加,细胞的增殖抑制率上升(P＜0.01);Hoechst染色结果显示,与对照组相比,共同孵育卫矛醇24 h后的C6细胞出现明显的核固缩现象,证明有大量的细胞出现凋亡;Western Blot结果显示,与对照组相比,促凋亡基因Bax表达上调,抗凋亡基因Bcl-2表达下调。结论:卫矛醇能够抑制C6细胞的增殖,并诱导其凋亡,其作用机制可能与上调Bax、下调Bcl-2有关。     

Sm-Nd ISOTOPIC AGE OF LAMPROPHYRES IN THE GEZHEN GOLD-BEARING SHEAR ZONE ON HAINAN ISLAND AND ITS GEOLOGICAL IMPLICATIONS

Sm-Nd isotopic compositions of eight lamprophyre samples, which come from the Gezhen gold-bearing shear zone on western Hainan Island, are measured. The Sm-Nd isochron age is 495.98±13.14 Ma, (143Nd/144Nd) 0=0.512094, εNd(t) ranges from +1.80 to +2.00 and TDM from 982 Ma to 1196 Ma (average: 1060 Ma). The authors point out that the whole-rock Sm-Nd isochron age (495.98 ± 13.14 Ma) really represents the petrogenetic age of lamprophyre and the time of magmatism during subsequent subduction.     

基于复模态的有限元模型修正算法

针对地下结构地震响应分析中无限地基辐射阻尼问题，引入复模态情况下的具有非简化的堆积阻尼矩阵的阻尼模型，并针对具有集中质量阵的阻尼模型提出了合并与质量有关的阻尼和堆积阻尼的思想，并据此提出了一种修正此类有限元模型的两步法，首先从复模态参数中提取实模态参数，采用基于模态残余力的识别算法修正刚度矩阵，然后根据复模态参数和已得的刚度矩阵来识别阻尼模型中的刚度参与系数和质量阻尼堆积阻尼联合矩阵。     

Study of charge structure and radiation characteristic of intracloud discharge in thunderstorms of Qinghai-Tibet Plateau

The comprehensive observations on lightning discharges were conducted in Naqu area of Qinghai-Tibet Plateau in summer of 2002. The electric structures of thunderstorms and the characteristics of lightning discharges at initial stage were analyzed by using the observation data. The results show that most of intracloud (IC) lightning flashes were polarities inverted in thunderstorms with tripole electric charge structure and occurred between negative charge region located in the middle of the thunderstorm and positive charge region located at the bottom of the thunderstorm. The radiation characteristics of discharge processes in cloud with longer lasting time involved in Cloud-to-Ground (CG) lightning flashes were similar to that of IC discharges.A lot of radiation pulses were produced in these discharge processes. Because the IC discharges took place at the bottom of thundercloud and were near the ground, they may produce more serious damage to equipment on the ground therefore should not be neglected in lightning protection.     

Sedimentary environment and development controls of the hydrocarbon source beds:Middle and Upper Proterozoic in northern North China

Problem on development control of marine source bed hold in Chinese petroleum industry progression. The Hongshuizhuang Formation,Tieling Formation and Xiamaling Formation in the Middle and Upper Proterozoic are important hydrocarbon source beds in northern North China, and investigation of their sedimentary environments and the controls has great significance for petroleum exploration in North China. Based on sedimentology (sequence stratigraphy), palaeoecology, sedimentary geochemistry, and sedimentary palaeogeography, their development pattern is discussed. All these studies indicate that the development controls of the hydrocarbon source beds include a favorite palaeogeographic location, exceeding propagation of biomes in low and middle latitudes, anoxic environments, enrichment of phosphorus element and the adsorption of clay minerals during the preservation of organic matter in the marine carbonates.     

运用改进系统建模法对南海气象数据的建模研究

在系统建模理论的基础上,运用改进的动态数据建模方法,对南海气象数据中的温度进行建模并验证了模型的适用性.根据模型推导出格林函数、逆函数和自协方差函数等,并讨论了南海气象数据中温度模型的稳定性、可逆性和合理性.对系统的频率特性和谱函数进行分析讨论,并给出建模过程中的一些图像.根据模型的适用性检验发现,对所研究的气象数据而言,ARMA(4, 3)模型是最合适的,具有平稳可逆性.所有的建模和分析过程在MATLAB上实现.实验结果表明这种建模方案简便易行,能够快速准确地确定系统的合理模型.     

人工神经网络在岩体质量分级中的应用

结合四川省金沙江某水电站工程实例，应用BP人工神经网络方法建立3层BP网络模型，选取岩石单轴抗压强度等6个影响因素为输入变量，对坝基复杂岩体进行质量分级。通过机算机Visual C 语言编程实现神经网络模型，进行网络的学习和运算。以神经网络合理结构分析方法选取合理结构，确定合理隐层单元的数量，提高网络测试的精度。对测试结果的分析发现，经过优化的BP网络模型经多次学习后，测试精度提高，结果可靠，取得较好的实际应用效果。     

基于GIS的孔隙水文地质层三维空间离散方法

针对自然界中孔隙水文地质层空间分布的不连续性与厚度分布的不均匀性,研究基于GIS的孔隙水文地质层三维空间离散实现的技术路线,提出基于GIS的孔隙水文地质层不规则六面体元的三维空间离散方法.该法不仅能最大限度地保证不规则六面体元中水文地质层类型的一元性,而且可充分利用GIS的空间分析与数据的自动提取功能,快速提取各个计算结点上空间位置坐标与各类计算参数,大大缩短水文地质模型空间离散与相关数据文件组织所需的时间,提高地下水三维有限差分数值模拟的时效性,具有较高的实用价值.     

采用DPIV技术研究浅水平板岛屿尾流

人们对于尾流的研究大都针对绕流体在展向非常长的情形,但是在天然环境和水利工程中出现的多为水深相对很浅的流动。由于槽底和自由水面的限制,浅水域中的尾流有着与深水尾流不同的流动规律。采用数字粒子图像测速(DPIV)技术,在长30m、宽3.5m、高1m的水槽中比较系统地量测了浅水中绕平板岛屿的尾流区流场。分析了大尺度旋涡的运动规律,得到了尾流流动形式随尾流稳定性参数的变化规律。目前如此大尺度浅水岛屿尾流的定量实验研究并不多见。     

青铜文物腐蚀过程中的次生矿物

产于不同环境不同地方的青铜文物经矿相显微镜观察、电子探针成分分析和X射线衍射研究表明，在青铜文物腐蚀层中观察到次生矿物——赤铜矿、孔雀石、蓝铜矿、白铅矿和锡石。由上述矿物组成的腐蚀层在青铜器表面形成保护层。腐蚀层细分为红色赤铜矿层、绿色碳酸盐矿物层和黄色锡石层组成，其厚度小于1mm。青铜合金在潮湿条件下的腐蚀比在干旱条件下要严重，另外青铜合金是适用于作高放废物地质处置系统中的废物罐的候选材质。