从商务日语课程设置谈高职教育课程改革

本科院校办高职学院是我国 2 0世纪末出现的一个新生事物 ,如何设置专业课程 ,提高教学质量 ,使学生一出校门就能容纳到直接为经济服务当中去 ,结合商务日语专业的具体情况对这个问题进行了思考。     

Ontogeny of the lymphoid organs of Japanese flounder, Paralichthys olivaceus

Histological study on the ontogeny of the lymphoid organs, kidney, thymus and spleen of Japanese flounder, Paralichthys olivaceus, from hatching to 40 d was carried out. The pronephric kidney duct appeared early in hatching although the primordial haemopoietic stem cells were observed within a week after hatching. The spleen was first seen after 8d of hatching. The thymus appeared after 15d, situated near the pronephric kidney. Small lymphoid cells appeared during the later phase of the post-larval stage in the sequence of thymus, kidney and spleen. During the 40d of observations, there were no distinct inner or outer zones in thymus and no red or white pulp in spleen. These results suggest that the nonspecific defense immune system plays a very important role in the early larval stage of Japanese flounder.     

CHARACTERIZATION OF LACTATE DEHYDROGENASE ISOZYME PATTERN AND MORPHOLOGY OF THREE MARINE FISH CELL LINES

Three continuous marine fish cell lines of FG (i. e., Hounder Gill) from flounder (Paralichthys olivaceus) gill, SPH (i. e. , Sea Perch Heart) from sea perch (Lateolabrax japonicus) heart and RSBF (i.e., Red Sea Bream Fin) from red sea bream (Pagrosomus major) fin, were characterized by lactate dehydrogenase (LDH) isozyme and morphological analysis. The LDH isozyme patterns of these three cell lines and their corresponding tissues of origin were investigated and compared. The results showed: (1) No difference was found in the LDH isozyme patterns of FG and flounder gill tissue. However, the LDH isozyme patterns of SPH and RSBF were significantly different from their corresponding tissues of origin; (2) LDH isozyme patterns of FG, SPH and RSBF were markedly different from each other and could serve as genetic markers for species identification and detection of cross contamination. Morphological change analysis of these three cell lines in comparison to their original tissues indicated that FG cells still appeared epithelioid without morphological transformation. However, morphological changes were found in SPH and RSBF compared to their original tissues. Therefore, the cellular morphology was still plastic in the relatively stable culture conditions, and it was possible that change of LDH patterns wasrelated to morphological changes of fish cells in vitro.     

杂交鲆(牙鲆♀×夏鲆♂)胚胎发育的初步观察

通过人工调控促使亲本牙鲆(Paralichthys olivaceus)♀×夏鲆(Paralichthys dentatus)♂的性腺发育,人工干法授精获得杂交受精卵。杂交受精卵在温度为16.5~17.5℃,光照为100~400 lx,微充气条件下孵化。对杂交鲆胚胎发育全过程进行连续观察并与牙鲆及夏鲆的胚胎发育进行比较。结果表明:杂交鲆卵裂方式与其他大部分硬骨鱼类一样,属于盘状卵裂。受精后经过2 h 30 min进入2细胞期,7 h 45 min进入囊胚期,15 h 10 min进入原肠胚阶段,25 h 30 min进入神经胚期,49 h左右进入尾芽期。授精后63 h左右发现初孵仔鱼,68h左右50%的仔鱼孵出。在杂交鲆的胚胎发育中,主要有2个发育特征与母本类似而与父本不同:视囊的出现要早于体节的分化;孵化前,仔鱼在卵膜内环绕卵黄囊不到1周。但是在其孵化时间上却与父本相似。     

利用牙鲆鳃细胞系分离和培养淋巴囊肿病毒

本文利用牙鲆鳃细胞系进行了养殖牙鲆淋巴囊肿病毒的分离及培养 ,并通过电镜对培养细胞中淋巴囊肿病毒的形态及感染循环进行了初步研究。将病鱼的淋巴囊肿组织无菌滤液接种牙鲆细胞系 ,细胞出现了明显的细胞病变 ( Cytopathic effect,CPE)。电镜观察在培养细胞的胞质中有病毒的包涵体 ,胞质中散在 6角形、5角形或圆形的病毒粒子 ,大小为 10 0～ 140 nm之间。在感染细胞的线粒体中也存在大量的病毒颗粒。     

Locomotor activity rhythm in the Japanese eel Anguilla japonica elvers

Under artificial LD cycles(6,12,18 L),the elvers of Japanese eel,Anguilla japonica,showed a 24 h cycle of locomotor activity rhythm being most active at light transitions:the eels' activity rose to a primary peak after lights-off,followed by a quiescent period during which they buried into the shelters or lying motionlessly on sand for most of the time,and then reached a secondary peak before lights-on.Elvers could resynchronize their activity rhythm with a new photo cycle within 4 d.Moreover,their activity level at dark phase significantly increased as the light period was prolonged:higher activity levels during shorter dark period.However,the elvers did not display clearly the existence of a circadian rhythm under constant light or dark conditions.The timing of daily activity rhythm evidenced in the Japanese eels may occur through the action of the LD cycles with a weak participation of an endogenous circadian system.In all the LD cycles,over 99% of the activity occurred in the dark phase,indicating that the eels were always nocturnally active no matter what time of day it might be.Under 12 L conditions,the eels' activity level and the time outside sand were significantly elevated both at light and dark phases as temperature increased from 10~15 to 20~25 ℃.The activity rhythm pattern(i.e.,two peaks occurring around light transitions) did not apparently change among temperatures.However,in contrast with the primary activity peaks immediately after lights-off at 20 and 25 ℃,the timing of the primary peaks at 10 and 15 ℃ showed a latency of a few hours following lights-off,indicating the inhibiting effect of low temperature on the eels' activity.     

石鲽鱼人工育苗技术研究

根据石鲽鱼的生物学特性 ,对亲鱼的选择培育、人工催产、挤卵、受精孵化和仔稚鱼的培育技术 ,进行试验探索。在室内人工控制水温、光照、饵料投喂的条件下 ,较为系统地研究了石鲽鱼的发育和变态及其与理化因子的关系 ,探索了人工育苗的关键技术 ,突破了过去单纯依赖采捕自然苗进行人工养殖的方式 ,为实现室内全人工育苗及养成产业化生产 ,解决苗种来源途径提供了技术保证     

大西洋牙鲆幼鱼肌肉组成与营养需求的探讨

测定了养殖大西洋牙鲆幼鱼肌肉中的蛋白质、脂类、灰分等生化组织,并计算了其比能值,分析了肌肉蛋白质中17种常见氨基酸的含量、脂肪酸组成及含量。结果表明:大西洋牙鲆属高蛋白、低脂肪、低比能值鱼类,肌肉氨基酸含量同其它鲆鲽鱼类相比,属中等水平,必需氨基酸和呈味氨基酸的含量较高;肌肉蛋白中氨基酸的支/芳值为2.794;脂肪酸含量中棕榈酸(16∶0)的含量最高,其次为油酸(18∶1 n)和DHA(22∶6 n-3);其EPA(20∶5 n-3)和DHA(22∶6 n-3)的含量较高,营养丰富且平衡良好,是值得推荐的优良养殖品种和食用鱼类。     

Issues of Globalization and Reflexivity in the Japanese Tourism Production System: The Case of Whistler,British Columbia

This article examines contemporary Japanese overseas tourism from a supplier‐side perspective using the concept of production systems. We first outline characteristics of the evolving structure of Japanese overseas tourism, with an emphasis on the global spread of Japanese travel companies. This provides a frame for presenting an empirical account of the transactional relationships in the Japanese package tour production system in Whistler, British Columbia, where Japanese tour operators play a pivotal role. We conclude that the recent expansion of Japanese travel companies is fostering the functional integration of the global tourism production system and exhibits increasing reflexivity.