Effects of probiotic on microfloral structure of live feed used in larval breeding of turbot Scophthalmus maximus

The effects of an exogenous probiotic( Bacillus amyloliquefaciens) on microbial community structure of B ranchionus plicatils and A rtemia sinica were evaluated in this study during turbot( Scophthalmus maximus) larval breeding. The analysis and comparison of the microfloral composition of live feed with probiotic was conducted using the Illumina HiSeq PE250. The abundance of microbial species and diversity of microflora in live feed with B. amyloliquefaciens were higher than those in the control. The microfloral composition was similar among the three replicate experimental groups of B. plicatils compared with the control after enrichment. L actococcus, Pseudoalteromonas, and Alteromonas were always dominant. Additionally, some other bacterial species became dominant during the enrichment process. The microbial community during nutrient enrichment of A. sinica was rather similar among the three control replicates. Relative abundance of Cobetia sp., the most dominant species, was 54%–65.2%. Similarity in the microbial community was still high after adding B. amyloliquefaciens. Furthermore, P seudoalteromonas and Alteromonas replaced Cobetia as the dominant species, and the abundance of Cobetia decreased to 4.3%–25.3%. Mean common ratios at the operational taxonomic unit level were 50%–60% between the two B. plicatils and A. sinica treatments. Therefore, the microbial community structure changed after adding B. amyloliquefaciens during nutrient enrichment of B. plicatils or A. sinica and tended to stabilize. Additionally, the abundance of V ibrio in any kind of live feed was not significantly dif ferent from that in the control. These results will help improve the microflora of B. plicatils and A. sinica and can be used to understand the multiple-level transfer role of probiotic species among probiotic products, microflora of live feed, and fish larvae.     

我国仅存一对北极海獭喜得“娇女”

8月31日上午9时20分, 大连老虎滩极垲海洋动牛旁绾2001年从北扳芎I琏-嫒馆直鲁鹰糨顺利地生下了第一个珍贵的“婴儿”一一海獭小公童。鹰糖妈蚂目“婴儿”幽生,就一直把它敢在怀里,一刻不停垲用舌獒为它梳理度毛,甚至乏记了i莲童。我国仅存一对北极海獭喜得“娇女”@林龙安 @肖淑女     

印支运动的沉积学响应——以湖北秭归盆地中、上三叠统为例

通过对秭归盆地南缘中、上三叠统巴东组和沙镇溪组地层剖面的实测及1∶10万秭归幅地质填图发现,中三叠统巴东组在秭归盆地东南缘存在不同程度的缺失,秭归郭家坝一带,只沉积巴东组一段、二段地层。秭归楠家湾一带,整个巴东组全部缺失,上三叠统沙镇溪组直接覆盖于早三叠世嘉陵江组之上。而秭归盆地西缘沙镇溪、巴东麂子岩一带,巴东组发育齐全。上述现象表明,在早三叠世末期,由于印支运动的影响,巴东组地层出现了较大差异。沙镇溪组在沉积上继承了巴东组高低不平的古地貌特征,沉积厚度变化较大。上三叠统沙镇溪组与中统巴东组之间,在盆地不同的部位可呈现出整合或平行不整合的接触关系,而不存在断层接触关系。     

影响低渗透油藏低速非线性渗流的实验研究

以膨胀性的粘土为主要研究对象,通过粘土浆体的流变性实验和填砂管渗流实验,并使用一种新研制的微流量测量工具,研究了低渗岩心在较低流速下的渗流特性;利用微尺度流动理论,Einstein粘度定律等进行了理论分析,对引起低渗透油藏低速非线性渗流的影响因素进行了初步的探索.结论认为,粘土的膨胀性以及其水化后形成浆体的流变性是引起低速非线性渗流的主要因素之一.     

用微机研究光电倍增管伏

光电倍增管的伏安特性系指，当投射到光阴极上的光强恒定时，阳极电流与总的工作电压之间的关系:     

沙质海床对波浪的响应及其稳定性研究的回顾

本文简要回顾了前人关于沙质海床在波浪作用下的响应，液化和稳定性等方面问题和研究状况。对各研究中所采用的计算模型、计算方法、应用范围以及存在的问题作了较为系统的评述。此外对各问题的最新研究成果也作了简要的介绍，最后讨论了该领域中有待于进一步研究的问题。     

曹淑媛的养虾经

1993年、1994年我国对虾养殖连续两年大面积欠产、绝产。大连市普兰店丰荣办事处五里台村养虾专业户曹淑媛饲养的虾圈却获得了空前的丰收。她是怎样获得了丰收的呢?记者日前采访了曹淑媛。这位名叫曹淑媛的女养虾专业户,今年46岁,原先是一位普通农村妇女。党的改革开放政策使她萌生了走出家门、离开锅台、干点事业的念头。她选择了养虾这个行     

中国对虾幼体期视觉器官的发育

于１９９４年３－５月，以中国对虾无节幼体６个亚期，蚤状幼体３个亚期，糖虾幼体３个亚期及仔虾各时期的幼体为材料，用日产Ｏｌｙｍｐｕｓ万能研究显微镜观察研究各期幼体单眼及复眼的发育。结果表明：在中国对虾无节幼体Ｎ１－Ｎ６６个亚期，单眼的两侧均有复眼的原基组织。     

要为海域划界早做准备

海洋里有丰富的资源,很多品种的储量超过陆地。海洋资源大体可分成几部分:1.海水资源。世界淡水资源日益紧张,人们的目光已转向极地冰和海水。目前,海水淡化成本还比较高,随着科技的进步,人类将能从海洋里大量提取淡水;农业也正在试用海水直接浇灌。同时,海水里含有多种矿物元素,盐化工工业就是以     

Experimental Study on Stability of Breakwaters with Penetrating Box Foundations

1 .ＩｎｔｒｏｄｕｃｔｉｏｎＴｈｅｐｅｎｅｔｒａｔｉｎｇｂｕｃｋｅｔａｎｄｂｏｘｆｏｕｎｄａｔｉｏｎｉｓａｎｅｗｔｙｐｅｏｆｓｔｒｕｃｔｕｒｅ ,ｗｈｉｃｈｈａｓｂｅｅｎａｐｐｌｉｅｄｉｎｍａｎｙｅｎｇｉｎｅｅｒｉｎｇｆｉｅｌｄｓ .Ｉｎｖｉｅｗｏｆｔｈｅｈｉｇｈｃｏｎｓｔｒｕｃｔｉｏｎｃｏｓｔａｎｄｔｈｅｆｏｒｍｉｄａｂｌｅｃｏｎｓｔｒｕｃｔｉｂｉｌｉｔｙｏｆｏｆｆ ｓｈｏｒｅｐｌａｔｆｏｒｍｓｗｉｔｈｐｉｌｅｄｆｏｕｎｄａｔｉｏｎｓ ,Ｂａｅｒｈｅｉｍｅｔａｌ.(1 990 )ｐｒｏｐｏｓｅｄｔｈｅｃｏｎｃ…