海南九孔鲍养成技术

自１９９３年我国皱纹盘鲍苗种及成体发生大规模死亡以来 ,北方鲍鱼产量一直处于低谷阶段 ,鲍鱼人工育苗及养殖严重受阻 ;１９９６年开始增产 ,但速度缓慢 ,生产极不稳定。南方福建东山一带 ,目前发生鲍鱼大规模死亡现象 ,并有向南方拓展之势 ,如广东湛江、汕头、汕尾也发生鲍鱼大规模死亡现象。海南省政府决定 ,今后几年内全省计划大力发展鲍类养殖 ,全省计划几年内发展网箱养鲍１０×１０５箱 ,许多企业大力开展工厂化养鲍。本文针对海南养殖九孔鲍的独特特点 ,从养殖模式及养成技术的操作和管理 ,以及养殖过程中存在的诸多问题作一简要的…     

Cloning of a chub metallothionein cDNA and development of competitive RT-PCR of chub metallothionein mRNA as a potential biomarker of heavy metal exposure

Metallothionein has been assayed in a range of aquatic animal tissues as an indicator of metal exposure. We sequenced chub (Leuciscus cephalus) metallothionein cDNA which showed over 90% homology to common carp, goldfish and stone loach and 77% homology to rainbow trout sequences for metallothionein. We then used the extended primer method to develop an accurate quantitative competitive RT-PCR assay for metallothionein mRNA. RT-PCR was used to measure metallothionein mRNA in feral chub from a range of field sites, with different levels of heavy metal pollution, in the West Midlands, UK. Measurements were complemented by analysis of liver and gill metallothionein protein by capillary electrophoresis. There was no significant difference in the metallothionein protein levels between fish of different rivers and there was no evidence of elevation of mRNA at the sites of highest metal exposure. The level of metal exposure (e.g. zinc, nickel and cadmium each ranging between 15 and 28 microg/l ) at the pH (7.5-8.5) of these rivers appears insufficient to elevate hepatic or gill metallothionein in chub. A lack of elevation of hepatic metallothionein mRNA in chub exposed to zinc, copper and manganese for 24 h and 10 days in the laboratory also suggests a non-responsiveness of this species.     

Correlative changes in metabolism and DNA damage in turbot (Scophthalmus maximus) exposed to benzo[a]pyrene

Juvenile turbot (Scophthalmus maximus) were injected intraperitoneally with either corn oil or 5 mg/kg benzo[a]pyrene (BaP) dissolved in corn oil and sampled I and 3 days after injection. After 1 day, no elevation of 7-ethoxyresorufin O-deethylase (EROD) activity was observed, however bile metabolites (BaP-7,8 dihydrodiol representing 70% of the total metabolites) and a single hepatic DNA adduct spot (0.47 adducts/10(8) nucleotides) identified by 32P-postlabelling were formed. No BaP metabolites or DNA adducts were observed in either control or carrier control fish. Fish sampled after 3 days reported 5-fold higher (P < 0.05) levels of EROD activity, a shift in the bile metabolite profile towards BaP phenol formation (1OH and 30H BaP comprising up to 60% of total metabolites detected) and the formation of two adduct spots (0.86 and 0.71 adducts/10(8) nucleotides). These results show that BaP can be metabolised and form hydrophobic DNA adducts in turbot without EROD elevation. Following EROD elevation, a shift in the profile of both BaP metabolites and BaP metabolite-DNA interactions occurs indicative of other oxidative processes.     

若尔盖县生活垃圾填埋场渗滤液回喷处理设计

结合当地蒸发量远大于降雨量的特点,在若尔盖县生活垃圾卫生填埋场渗滤液污染控制中采用了渗滤液循环回喷处理技术,可实现在填埋运营期渗滤液不外排,同时,结合工程设计,介绍了渗滤液产量的计算方法、处理系统的组成、回喷系统的设计以及构筑物设计等情况.     

海洋浮游微食物网结构及其影响因素

微食物环是海洋生态系统中重要的物质和能量过程,是传统食物链的有效补充。微食物环研究是当前海洋生态学研究的热点之一,但对其结构的系统研究较少,海洋微食物网结构在2000年才被Garrison提出。尽管微食物网各个类群的丰度在不同海洋环境中有相对变化,但是这些变化都处于一定的范围之内,其丰度结构约为纤毛虫10 cell ml-1、鞭毛虫103 cell ml-1、微微型真核浮游生物104 cell ml-1、蓝细菌104-5 cell ml-1、异养细菌106 cell ml-1、病毒107 particle ml-1。海洋浮游食物链中捕食者和饵料生物粒径的最佳比值为10:1,实际研究中该比值会略低,例如纤毛虫与其饵料的粒径比值为8:1,鞭毛虫为3:1。Pico和Nano浮游植物的丰度比（Pico:Nano）是研究微食物网结构的指数之一,该指数具有不受研究尺度影响的优点,可用于研究区域性和全球性微食物网结构。近年来,学者们从多角度对海洋微食物网的结构开展了研究,不同海区微食物网各类群丰度、生物量的时间和空间变化研究有很多报道,微食物网的结构可受空间、季节、摄食、营养盐等多种因素影响。在对不同空间微食物网的研究中,学者往往研究不同物理性质的水团中各类群生物丰度的不同,以此来表征微食物网结构的不同;同一海区微食物网结构的季节变化也是使用各个类群丰度和生物量的变化来表示,该变化主要受水文环境因素影响。摄食者对微食物网各类生物的影响通过三种途径：1. 中型浮游动物摄食;2. 中型浮游动物摄食微型浮游动物,通过营养级级联效应影响低营养级生物;3. 中型浮游动物通过释放溶解有机物、营养盐影响细菌和低营养级生物。浮游植物通过产生化感物质和溶解有机物影响微食物网结构,而营养盐的浓度及变化则可以对微食物网产生直接或间接影响。     

利用二次离子质谱分析珊瑚的氧同位素组成

本文针对二次离子质谱分析珊瑚氧同位素组成过程中存在的非规律性基体效应问题,提出多控制点分段计算并分段校正珊瑚的氧同位素分馏方法。为验证该方法的有效性,我们对比了原位微区分析的珊瑚δ~(18)O_(PDB)和磷酸法分析对应的月分辨率的珊瑚粉末氧同位素结果,两者展示了很好的相关性,说明本文提出的校正方法可以校正样品差异带来的基体效应,能够简单并有效地提高珊瑚微区氧同位素的准确度。该方法对研究珊瑚中氧同位素分馏机理、评估珊瑚生命效应的影响以及利用氧同位素重建高分辨率古气候记录的合理性具有重要意义。     

改进的混合像元分解法用于快速水污染遥感评价研究

该文介绍了一种改进的混合像元分解方法。利用该方法对滇池的各类污染（叶绿素,营养盐等）进行快速评价,可以直接得出滇池各个区域所受各类污染的程度,从而给出滇池全区各类污染的情况分布。