栉孔扇贝foxl2基因在卵子发生中的必要性

采用RNAi技术研究了栉孔扇贝(Chlamysfarreri)foxl2基因在卵子发生和卵巢功能维持中的作用。使用体外合成的栉孔扇贝foxl2双链RNA(dsRNA),以每只50μg/次的剂量注射进闭壳肌中,连续注射2次(第一次注射后7天,再进行第二次注射);以注射相同体积PBS(相同注射方法)的扇贝作为阴性对照组,以不注射扇贝作为空白对照组。qRT-PCR检测干扰组扇贝卵巢中的foxl2 mRNA表达量较空白对照组下降了62%, Western blotting检测该蛋白在卵巢中的含量也明显下降,显示靶基因的表达水平被有效地敲降。组织学观察发现,栉孔扇贝foxl2干扰后的卵巢中卵母细胞形态异常、细胞核固缩,卵子发生明显受阻。表明该基因在栉孔扇贝卵子发生中起重要作用。     

Cloning of a chub metallothionein cDNA and development of competitive RT-PCR of chub metallothionein mRNA as a potential biomarker of heavy metal exposure

Metallothionein has been assayed in a range of aquatic animal tissues as an indicator of metal exposure. We sequenced chub (Leuciscus cephalus) metallothionein cDNA which showed over 90% homology to common carp, goldfish and stone loach and 77% homology to rainbow trout sequences for metallothionein. We then used the extended primer method to develop an accurate quantitative competitive RT-PCR assay for metallothionein mRNA. RT-PCR was used to measure metallothionein mRNA in feral chub from a range of field sites, with different levels of heavy metal pollution, in the West Midlands, UK. Measurements were complemented by analysis of liver and gill metallothionein protein by capillary electrophoresis. There was no significant difference in the metallothionein protein levels between fish of different rivers and there was no evidence of elevation of mRNA at the sites of highest metal exposure. The level of metal exposure (e.g. zinc, nickel and cadmium each ranging between 15 and 28 microg/l ) at the pH (7.5-8.5) of these rivers appears insufficient to elevate hepatic or gill metallothionein in chub. A lack of elevation of hepatic metallothionein mRNA in chub exposed to zinc, copper and manganese for 24 h and 10 days in the laboratory also suggests a non-responsiveness of this species.     

Correlative changes in metabolism and DNA damage in turbot (Scophthalmus maximus) exposed to benzo[a]pyrene

Juvenile turbot (Scophthalmus maximus) were injected intraperitoneally with either corn oil or 5 mg/kg benzo[a]pyrene (BaP) dissolved in corn oil and sampled I and 3 days after injection. After 1 day, no elevation of 7-ethoxyresorufin O-deethylase (EROD) activity was observed, however bile metabolites (BaP-7,8 dihydrodiol representing 70% of the total metabolites) and a single hepatic DNA adduct spot (0.47 adducts/10(8) nucleotides) identified by 32P-postlabelling were formed. No BaP metabolites or DNA adducts were observed in either control or carrier control fish. Fish sampled after 3 days reported 5-fold higher (P < 0.05) levels of EROD activity, a shift in the bile metabolite profile towards BaP phenol formation (1OH and 30H BaP comprising up to 60% of total metabolites detected) and the formation of two adduct spots (0.86 and 0.71 adducts/10(8) nucleotides). These results show that BaP can be metabolised and form hydrophobic DNA adducts in turbot without EROD elevation. Following EROD elevation, a shift in the profile of both BaP metabolites and BaP metabolite-DNA interactions occurs indicative of other oxidative processes.     

EVALUATION OF DAILY PRECIPITATION IN CHINA FROM ECMWF AND NCEP REANALYSES

ＩＮＴＲＯＤＵＣＴＩＯＮＷｅａｔｈｅｒｆｏｒｅｃａｓｔｉｎｇａｓａｍａｊｏｒｓｕｂｊｅｃｔｉｎａｔｍｏｓｐｈｅｒｉｃｓｃｉｅｎｃｅｈａｓｄｅｖｅｌｏｐｅｄｓｉｎｃｅｔｈｅ 1 950’ｓｉｎｔｏａｍｏｄｅｒｎｓｃｉｅｎｃｅ .Ｎｕｍｅｒｉｃａｌｗｅａｔｈｅｒｆｏｒｅｃａｓｔｍｏｄｅｌｓａｒｅｅｘｔｅｎｓｉｖｅｌｙａｎｄｆｒｅｑｕｅｎｔｌｙｕｓｅｄｔｏｃｈｅｃｋｔｈｅｔｈｅｏｒｉｅｓ,ｒｕｌｅｏｕｔｔｈｅｏｌｄｉｎｃｏｒｒｅｃｔｏｎｅｓａｎｄｐｒｅｓｅｎｔｎｅｗｉｄｅａｓ,ａｎｄｓｕｇｇｅｓｔｍｅｔｈｏｄｓ…     

吉林四平山门银矿区韧性前切带构造变形特征及研究意义

通过对吉林四平山门银矿区韧性剪切带的宏观和显微构造变形特征的研究，认识到剪切带主要由钙质和长英质两类糜棱岩系列岩石组成，具左旋剪切，剪切总位移量约为８３０ｍ，用石英自由位错密度估算出差异应力值为７９．８－１２１．９ＭＰａ。该剪切带的加深了对山门银矿成矿作用、成矿条件及成矿规律的认识，对指导找矿具有重要意义。     

利用国土数值情报和卫星遥感资料分检霜冻危险地带

一、引言与农业有关的气象灾害危害的范围一般都比较大,进行灾害预测和灾情调查需要相当大的费用和时间。为此,近年来曾有人尝试利用遥感遥测技术进行农业气象灾害调查研究。到目前为止,这些研究大多局限于灾情调查分析方面,发展到灾害预测的研究还比较少。最近,国土地理院提供的国土数值情报把关于国土开发、利用的地理情报处理成计算机可以使用的数值或记号,利用这些数值资料可以迅速地进行地形判读,也可以很容易地用于受灾地区的地形分析。另一方面,气象卫星提供了从可见光到红外波段带域的