Molecular identification of bloom-forming species Phaeocystis globosa (Pryninesiophyta) and its dispersal based on rDNA ITS sequence analysis

The colony-forming Phaeocystis species are causative agents of dense bloom occurrences in coastal waters worldwide. It is difficult to separate them because of the different morphologies associated with their colonial stages. In this study we applied molecular approaches to analyze the genetic variation of Phaeocystis globosa and Phaeocystis pouchetii from several geographic regions, and to assist in tracing the dispersal of bloom-forming Phaeocystis species in coastal waters of China. The sequences of the internal transcribed spacers (ITS1 and ITS2) of rDNA and the 5.8S ribosomal RNA gene of Phaeocystis strains were determined. Sequence comparison shows that P.globosa was the most divergent to P. pouchetii, exhibiting sequence divergence higher than 0.08. However, lower genetic divergences existed between strains of P.globosa. The sequence comparison of the Phaeocystis rDNA ITS clearly shows that the species isolated from the southeast coast of China is identified as P.globosa rather than P. cf. pouchetii or other species. Furthermore, the significance of rDNA variation in distinct global populations of P.globosa suggested it might have had sufficient time to accumulate detectable mutations at the rDNA locus, supporting the hypothesis of ancient dispersal of P.globosa to many areas, meaning that P.globosa blooms in the coastal waters of China are endemic rather than a newly introduced species or a foreign source. Finally, based on the high divergent region of rDNA ITS, a pair of species-specific primers for P.globosa were designed, they could be useful to detect the presence of this species in mixed plankton assemblages or flagellate stages that are recognized with diffculties by means of conventional microscopy.     

三疣梭子蟹全人工工厂化育苗技术

2000年笔者在日照市水产研究所利用1450m^3水进行梭子蟹全人工工厂化育苗生产，共出Ⅱ-Ⅲ期幼蟹512．7万只，创造了出苗量为3500只／m^3，总产值为150多万元的直接经济效益，同时对幼体培育的密度进行了初步试验，结果表明：从Z1至M期幼体培育密度10－15万／m^3为宜，M期以后密度以0．8－1．5万／m^3为宜。     

Fatigue Behavior of Plain Concrete Under Biaxial Compression: Experiments and Theoretical Model

The effects of different lateral confinement stress on the fatigue behavior of and cumulative damage to plain concrete are investigated experimentally. Eighty 100 mm x 100 mm x 100 mm specimens of ordinary strength concrete are tested under constant- or variable-amplitude fatigue loading and lateral confinement pressure in two orthogonal directions. A fatigue equation is developed by modifying the classical Aas-Jakobsen S-N equation for taking into account the effect of the confined stress on fatigue strength of plain concrete. The results of variable-amplitude fatigue tests indicate that the linear damage theory proposed by Palmgren and Miner is unreasonable in the biaxial stress state. A nonlinear cumulative damage model that could model the effects of the magnitude and sequence of variable-amplitude fatigue loading and lateral confinement pressure is proposed on the basis of the evolution laws of the residual strains in the longitudinal direction during fatigue tests. The residual fatigue life predic     

An Experimental Study of Several Special Issues Regarding the Ice Force on Conical Structures

The effect of the cone spacing of a conieal structure on the ice force is studied by model experiments. The ice force reduction coefficient presented in this paper expresses the relationship between the ice force and the arrangement of cones. The experiments prove that the mode of the ice failure before the boundary of upward-downward bending cone (UDBC) is crushing. A conclusion can also be drawn from the experiments that the ice force on the boundary of UDBC is by far less than that on a vertical pile with the same diameter. Moreover, the ice force frequencies on upright and inve-rted cones are obtained under the field condition of the platform JZ20-2, respectively. The results show that the alterna-tion of the ice force on UDBC can hardly induce resonance of platform JZ20-2.     

Model Test Study of Dynamic Ice Force on Compliant Conical Structures

To study ice-induced vibration of a compliant conical structure,a series of model tests were performed from 2004 to 2005.In the tests,the ice sheet before the compliant conical structure was found to fail in two-time breaking.From 2005 to 2006,this type of ice failure was studied through more groups of tests.The tests show that two-time breaking is the typical failure of ice before steep conical structures,and is controlled by other factors at the same time,such as ice speed and the angle of the cone.     

Preliminary study on different nutrient pools supplies for the phytoplankton growth in the Jiaozhou Bay in China in the fall of 2004

The source and significance of two nutrients, nitrogen and phosphorous, were investigated by a modified dilution method performed on seawater samples from the Jiaozhou Bay, in autumn 2004. This modified dilution method accounted for the phytoplankton growth rate, microzooplankton grazing mortality rate, the internal and external nutrient pools, as well as nutrient supplied through remineralization by microzooplankton. The results indicated that the phytoplankton net growth rate increased in turn from inside the bay, to outside the bay, to in the Xiaogang Harbor. The phytoplankton maximum growth rates and microzooplankton grazing mortality rates were 1.14 and 0.92 d-1 outside the bay, 0.42 and 0.32 d-1 inside the bay and 0.98 and 0.62 d-1 in the harbor respectively. Outside the bay, the remineralized nitrogen (Kr=24.49) had heavy influence on the growth of the phytoplankton. Inside the bay, the remineralized phosphorus(Kr=3.49) strongly affected the phytoplankton growth. In the harbor, the remineralized phosphorus (Kr=3.73) was in larger demand by phytoplankton growth. The results demonstrated that the different nutrients pools supplied for phytoplankton growth were greatly in accordance with the phytoplankton community structure, microzooplankton grazing mortality rates and environmental conditions. It is revealed that nutrient remineralization is much more important for the phytoplankton growth in the Jiaozhou Bay than previously believed.     

神经网络反演散射计风场算法的研究

建立了一个神经网络反演卫星散射计海面风场的B-P算法,给出了一个神经网络反演风场的模型,并利用该反演算法和模型对实际卫星散射计数据进行了海面风场反演试验,对风向的多解性利用圆中数滤波方法进行排除.对神经网络训练和检验数据集分别采用ERS-1/2散射计数据和欧洲中期天气预报(ECMWF)提供的风场作为配准点数据.把反演的风速和风向与CMCD4和ECMWF的风场作了比较,它们吻合得比较好;研究表明神经网络反演海面风场是可行和高效的.     

Ecopath with Ecosim在水生生态系统研究中的应用

地球上的水体为人类提供着无法计量的生态系统服务和相应的生态价值。然而,水生生态系统一直承受着巨大的捕捞和环境压力。为更好地分析人类以及自然扰动对水生生态系统的影响,并建立科学合理的管理机制,许多研究种群结构和动态的模型软件以     

山东蓬莱大黑山岛碱性火山岩地球化学特征

庙岛群岛中的大黑山岛出露碱性火山岩,对研究壳-幔过程及黄、渤海演化具重要意义。文中作者对大黑山岛火山岩的常、微量元素、K-Ar年龄及Nd-Sr-Pb同位素体系进行测试确定。结果表明,岩石属钠质碱性玄武岩系列,岩浆岩喷发可分为8Ma前和8Ma后两个阶段;岩石常量元素含量除K2O、Na2O外差异小;随着火山岩的演化,岩浆源区深度变大;大黑山火山岩Nd-Sr-Pb同位素比值与夏威夷洋岛玄武岩相似,其岩浆的地幔端元组分为PREMA DM,又以PREMA为主,并有MORB物质混入。     

济阳坳陷博兴洼陷西部沙三段层序地层

选取以基准面为参照面的高分辨率层序地层学的理论与分析技术,对博兴洼陷西部沙三段开展层序地层分析工作。在博兴洼陷沙三段识别出5个层序界面和4个较大规模的洪泛面,由此将研究层段划分为4个长期基准面旋回(相当于3级层序),并通过长期旋回内部次级转换面的识别,细分出8个中期旋回(大致相当于4级层序)。通过对比建立了研究区的高分辨率层序地层格架,并分析了各层序的地层发育特征。以层序格架为基础,探讨了研究区各层序的沉积演化特征,建立了辫状三角洲—浊积扇层序发育模式,认为研究区辫状三角洲和浊积扇均具有加积作用特点;斜坡区为辫状三角洲发育区,而洼陷区为浊积扇发育区;中期基准面旋回下降期辫状三角洲发育,上升期浊积扇发育;浊积扇体的发育规模与湖泛规模相关。综合分析认为,浊积扇是形成岩性圈闭的主要储集砂体类型,其发育的有利层位是MSC8、MSC7、MSC6、MSC5旋回的上升半旋回,岩性圈闭发育的有利区是博兴南部斜坡坡折带之下的洼陷区。