pH对4种鲷鱼精子活力的影响

用不同ｐＨ的海水对真鲷（Ｐａｇｒｏｓｏｍｕｓ ｍａｊｏｒ）、平鲷（Ｒｈａｂｄｏｓａｒｇｕｓ ｓａｒｂａ）、黑鲷（Ｓｐａｒｕｓ ｍａｃｒｏｃｅｐｈａｌｕｓ）和黄鳍鲷（Ｓ．ｌａｔｕｓ）精子进行激活实验。结果表明，四种鲷鱼精子对ｐＨ的适应范围较广，其最适ｐＨ值与其生态习性相关。     

南海区4种鲷鱼精子的适盐性比较

研究了盐度对黄鳍鲷Ｓｐａｒｕｓ ｌａｔｕｓ Ｈｏｕｔｔｕｙｎ,平鲷Ｒｈａｂｄｏｓａｒｇｕｓ ｓａｒｂａ９Ｆｏｒｓｋａｌ）,黑鲷Ｓｐａｒｕｓ ｍａｃｒｏｃｅｐｈａｌｕｓ９Ｂａｓｉｌｅｗｓｋｙ）和真鲷Ｐａｇｒｏｓｏｍｕｓ ｍａｊｏｒ（Ｔｅｍｍｉｎｃｋ ｅｔ Ｓｃｈｌｅｇｅｌ）４种鲷鱼精子活力的影响。对南海区４种鲷鱼精子的适盐性及其活力进行了比较,黄鳍鲷,平鲷,黑鲷和真鲷精子激活所需的最低盐度分别为８,     

南海区4种鲷鱼精子的适盐性比较

研究了盐度对黄鳍鲷SparuslatusHouttuyn、平鲷Rhabdosargussarba（Forskal）、黑鲷Sparusmacrocephalus（Basilewsky）和真鲷Pagrsomusmajor（TemmincketSchlegel）4种鲷鱼精子活力的影响。对南海区4种鲷鱼精子的适盐性及其活力进行了比较,黄鳍鲷、平鲷、黑鲷和真鲷精子激活所需的最低盐度分别为8、10、＞8、＞10;激活的最适盐度则分别为21、22、25、25,与之相应的精子活动（涡动）时间分别为14．7、17．7、17．2、18．0min;在高盐条件（盐度40左右）下,黄鳍鲷精子的适应性最强,精子活力最好,其次是黑鲷、真鲷的精子,平鲷精子对高盐条件的适应性最差。     

EFFECTS OF RATION, TEMPERATURE AND BODY WEIGHT ON METABOLISM IN YOUNG BLACK PORGY

ＩＮＴＲＯＤＵＣＴＩＯＮＴｈｅｍｅｔａｂｏｌｉｓｍｉｎｆｉｓｈｉｎｃｌｕｄｅｓｓｔａｎｄａｒｄｍｅｔａｂｏｌｉｓｍ(ＲＳ)ｒｏｕｔｉｎｅｍｅｔａｂｏｌｉｓｍ(ＲＲ),ｓｐｅｃｉｆｉｃｄｙｎａｍｉｃａｃｔｉｏｎ(ＳＤＡ)ａｎｄａｃｔｉｖｅｍｅｔａｂｏｌｉｓｍ(ＲＡ),ｒｅｌａｔｅｄａｓ:　　ＲＴ=ＲＳ ＲＲ ＳＤＡ ＲＡｗｈｅｒｅＲＳｉｓｔｈｅｍｅｔａｂｏｌｉｓｍｏｆｔｈｅｆｉｓｈａｔｒｅｓｔ;ＲＲｔｈｅｍｅｔａｂｏｌｉｓｍｏｆｔｈｅｒｏｕｔｉｎｅｌｙａｃｔｉｖｅｆｉｓｈ;ＳＤＡｔｈｅｍｅｔａｂｏｌｉｓｍｏｆｔｈ…     

STUDY ON THE MODEL FOR PREDICTING SOIL EROSION AND ITS APPLICATION IN ARID AREA

１　ＴＨＥＡＮＡＬＹＳＩＳＯＦＳＯＩＬＥＲＯＳＩＯＮＦＡＣＴＯＲＳＴｈｅｉｎｔｅｎｓｉｔｙｏｆｓｏｉｌｅｒｏｓｉｏｎｉｎａｒｉｄａｒｅａｖａｒｉｅｓｗｉｔｈａｌｔｉｔｕｄｅ，ｍｏｉｓｔｕｒｅａｎｄｈｅａｔｃｏｎｄｉｔｉｏｎａｎｄｓｕｒｆａｃｅｌａｙｅｒ（Ｃｈｅｎ，１９９５）．Ｉｎｍｉｄｄｌｅａｎｄｈｉｇｈｍｏｕｎｔａｉｎｓｅｃｔｉｏｎｓ，ｔｈｅｒｅｉｓａｌｉｔｔｌｅｍｏｒｅｐｒｅｃｉｐｉｔａｔｉｏｎ（４５０－７９６．９０ｍｍ），ｒｉｃｈｅｒｖｅｇｅｔａｔｉｏｎｃｏｖｅｒｍｏｒｅｔｈａｎ６０ｐｅ…     

STUDY ON VEGETATION AND CLIMATE CHANGES IN BEIJING REGION SINCE LATE PLEISTOCENE

１　ＩＮＴＲＯＤＵＣＴＩＯＮＳｉｎｃｅｔｈｅ１９８０ｓａｓｅｒｉｅｓｏｆｒｅｓｅａｒｃｈｐｌａｎｉｎｃｌｕｄｉｎｇＩＧＢＰ（ｔｈｅＩｎｔｅｒｎａｔｉｏｎａｌＧｅｏｓｐｈｅｒｅ．ＢｉｏｓｐｈｅｒｅＰｒｏｇｒａｍｍｅ）ｈａｖｅｂｅｅｎｓｅｔｕｐ，ｏｆｔｈｅｍ，ＰＡＧＥＳ（ｔｈｅＰａｓｔＧｌｏｂａｌｃｈａｎｇｅｓ）ｉｓａｎｉｍｐｏｒｔａｎｔｃｏｒｅｐｒｏｊｅｃｔ．Ｔｈｅｎａｔｕｒｅｒｅｃｏｒｄｓｏｆｏｃｅａｎｄｅｐｏｓｉｔ，ｔｅｒｒｅｓｔｒｉａｌ（ｌｏｅｓｓ，ｌａｋｅｓａｎｄｓｗａｍｐ）ｄｅｐｏｓｉ…     

GROWTH OF NEW DESIGNATED CITIES IN CHINA

１ＩＮＴＲＯＤＵＣＴＩＯＮＭａｎｙｗｅｓｔｅｒｎｓｃｈｏｌａｒｓｓｕｃｈａｓＣｈａｎｇＳｅｎｄｏｕ（１９６３），ＣｈｅｎｇＴｉｅｊｕｎｅｔａｌ．（１９９４），ａｎｄＳｋｉｎｎｅｒＧ．Ｗ．ｅｔａｌ（１９７７）ｐｕｔｔｈｅｉｒｒｅｓｅａｒｃｈｉｎｔｅｒｅ...     

兴奋性毒性与神经和精神疾病的关系

根据Ｏｌｎｅｙ及其同事［１］以及更早的Ｌｕｃａｓ、Ｎｅｗｈｏｕｓｅ和其他研究者的工作，Ｏｌｎｅｙ在７０年代提出了兴奋性毒性（ｅｘｃｉｔｏｔｏｘｉｎ）的原始概念及兴奋性毒性这一专门术语。这些工作认为过量的兴奋性氨基酸（ｅｘｃｉｔａｔｏｒｙａｍｉｎｏａｃｉｄ，ＥＡＡ）─—谷氨酸（ｇｌｕｔａｍｉｃａｃｉｄ，ＧＬＵ）、ＧＬＵ的类似物门冬氨酸（ａｓｐａｒｔｉｃａｃｉｄ，ＡＳＰ）等─—能过度兴奋中枢神经系统（ｃｅｎｔｒａｌｎｅｒｖｏｕｓｓｙｓｔｅｍ，ＣＮＳ）内的ＧＬＵ敏感神经元而达到使它们致死的程度。在过去…     

THE CHINESE ECOLOGICAL RESEARCH NETWORK

ＴＨＥＣＨＩＮＥＳＥＥＣＯＬＯＧＩＣＡＬＲＥＳＥＡＲＣＨＮＥＴＷＯＲＫＺｈａｏＪｉａｎｐｉｎｇ（赵剑平）（ＢｕｒｅａｕｏｆＲｅｓｏｕｒｃｅｓａｎｄＥｎｖｉｒｏｎｍｅｎｔａｌＳｃｉｅｎｃｅｓ，ｔｈｅＣｈｉｎｅｓｅＡｃａｄｅｍｙｏｆＳｃｉｅｎｃｅｓ，Ｂｅ...     

花尾胡椒鲷肝脏的显微结构

花尾胡椒鲷 (Ｐｌｅｃｔｏｒｈｙｎｃｈｕｓｃｉｎｃｔｕｓ)为海水真骨鱼类 ,我国沿海均有分布 ,现已成功实现鱼苗人工繁殖 ,是一类具有较高营养价值的海水经济鱼类。国内外对鱼的内脏组织结构的研究主要集中在淡水鱼 ,对海水鱼研究较少。笔者在对花尾胡椒鲷肝脏进行了较详细解剖后 ,对其肝脏的显微结构进行了研究 ,为深入开展花尾胡椒鲷组织学与生理学研究提供资料。1　材料和方法花尾胡椒鲷采自广东省湛江市霞山东风市场 ,体重 0 5ｋｇ ,体长 2 5～ 3 0ｃｍ。买回当天即胸腔解剖 ,取下完整的肝脏材料 ,用Ｂｏｕｉｎ氏液固定 ,常规石蜡包…     

4种鲷科鱼种内细胞色素b基因序列的保守性

对真鲷 (Pagrosomusmajor)、黄鳍鲷 (Sparuslatus)、黑鲷 (S .macrocephalus)和平鲷 (Rhabdosar gussarba )的线粒体DNA细胞色素b 4 0 5bp序列进行测定。结果发现 ,4种鲷科鱼种内碱基的变异较低 ,真鲷为 0 .2 5% ,黄鳍鲷为 0 .74 % ,黑鲷和平鲷均为 0 ;真鲷有 4种单倍型 ,黄鳍鲷有 2种单倍型 ,黑鲷和平鲷分别为 1种单倍型 ,且单倍型间变异位点很少 ,真鲷有 3个变异位点 ,黄鳍鲷仅有 1个变异位点 ,而黑鲷和平鲷无变异位点。结果表明 ,细胞色素b基因在这 4种鲷科鱼种内是相当保守的。     

Spermiogenesis in the yellowfin porgy (Sparus latus Houttuyn), with emphasis on the associated mitochondrion

Transmission electron microscopy of the yellowfin porgy (Sparus latus Houttuyn) spermatozoa ultrastructure showed the spermatozoon as a primitive type made up of the acrosomeless head, the flagellum, and the midpiece, at the periphery of which was a relatively big mitochondrion with more complex structure. It was found that during spermiogenesis, only one relatively big mitochondrion occurred in both the spermatid and the spermatozoon. This is different from other teleost fishes. During spemiogenesis, the mitochondria number is one, and morphology did not change. All these are different from those of other fishes.     

SPERMIOGENESIS IN THE YELLOWFIN PORGY (SPARUS LATUS HOUTTUYN), WITH EMPHASIS ON THE ASSOCIATED MITOCHONDRION

ImODUcrlONTheultmetruCtureofthespermatosoainteeostfisheshaddridesedtalandcomParedpelllard,l97o,Mattei,l97O).ComPatalwithotherVertebrausryisheIson,l995),FisheShavegreaterdriversityinspermatosoaultfastruCture,indudingthemorphologicalcharaCteristicaofthesperm-headsophnsteinerandPatZner,l99O),thefonnswithorwithoutacrosorapeisheIson,l995),thenumberoftheflagellum,andthedistinguishingfeaturesofAntochondrialderivatives(JawhesonandGrier,l993).AllthesearereataltotheFishposi-tioninevolutionandt…     

Effect of n-3 HUFA levels in rotifers and Artemia on growth and survival of larval black sea bream （Sparus macrocephalus）

Requirement for dietary n-3 HUFA (n-3 highly unsaturated fatty acid) for growth and survival of black sea bream (Sparus macrocephalus) larvae was studied using rotifers and Artemia at various levels of n-3 HUFA. Five treatments with rotifers and Artemia differing in n-3 HUFA were prepared by enriching them with various oil emulsions. Results indicated that dietary n-3 HUFA significantly influence fish n-3 HUFA levels and are essential for growth and survival of black sea bream larvae. The results also indicated that the incorporation of n-3HUFA TG (triacylglycerols) into tissues of larval black sea bream was more effective from natural fish oil in comparison with n-3 HUFA fatty acid ethyl esters from ethyl-esterified oil.     

Effect of n?3 HUFA levels in rotifers and Artemia on growth and survival of larval black sea bream ( Sparus macrocephalus )

Requirement for dietary n−3 HUFA (n−3 highly unsaturated fatty acid) for growth and survival of black sea bream (Sparus macrocephalus) larvae was studied using rotifers andArtemia at various levels of n−3 HUFA. Five treatments with rotifers andArtemia differing in n−3 HUFA were prepared by enriching them with various oil emulsions. Results indicated that dietary n−3 HUFA significantly influence fish n−3 HUFA levels and are essential for growth and survival of black sea bream larvae. The results also indicated that the incorporation of n−3HUFA TG (triacylglycerols) into tissues of larval black sea bream was more effective from natural fish oil in comparison with n−3 HUFA fatty acid ethyl esters from ethyl-esterified oil. Project No. 39770588 supported by the NSFC.     

大口黑鲈抗菌肽hepcidin cDNA序列和结构分析

以大口黑鲈为材料,提取肝脏总RNA,经RT-PCR扩增出hepcidin cDNA的开放阅读框(ORF)及3′端非编码区序列,应用5′RACE方法得到大口黑鲈hepcidin cDNA5′末端。将所获得的两个片段分别克隆到T载体后进行测序,并拼接成大口黑鲈hepcidin全长cDNA。序列分析表明:大口黑鲈hepcidin全长cDNA为564bp,含有一个258bp的ORF,编码86个氨基酸残基,由信号肽(24个残基)、前肽(42个残基)和成熟肽(20个残基)3部分组成hepcidin前体。在前肽部分具有前肽转化酶典型的RX(K/R)R基元,成熟肽部分含有8个保守的半胱氨酸残基,可形成四个链内二硫桥,使β-折叠结构保持稳定。大口黑鲈Hepcidin与其他鱼类的同源性在29.7%~90.5%间,尤其是信号肽区域,与鳜、尼罗罗非鱼、真鲷、花鲈、黑鯛、金眼狼鲈仅有2~3个氨基酸的差别。     

不确定性对北部湾二长棘鲷渔业资源评估影响的模拟研究

利用蒙特卡罗模拟方法研究了生物学参考点F0.1、Fmax和当前捕捞死亡系数(current fishing mortality rate,Fcur)估计中的不确定性对北部湾二长棘鲷渔业资源评估的影响。结果表明:二长棘鲷渔业资源状态评估受到F0.1、Fmax、Fcur估计中的不确定性和管理者选取的决策置信水平的影响,二长棘鲷渔业被确定为过度捕捞的概率随着F0.1、Fmax、Fcur估计中不确定性和置信水平的增加而下降;F0.1和Fmax估计中的不确定性主要由生长参数的不确定性引起,自然死亡系数的不确定性对F0.1和Fmax估计中的不确定性的影响是有一定限度的。相比Fmax,将F0.1作为二长棘鲷渔业的管理目标更为合适。     

Effect of ration, temperature and body weight on growth of black porgy,Sparus macrocephalus (B.)

Growth of black porgy,Sparus macrocephalus, was studied experimentally at four ration levels from starvation to satiation and four temperatures ranging from 14.8 to 26.8°C. At maximum rations, the specific growth rate (SGR) increased with temperature and was a negative linear function of body weight. The relationship between SGR and ration levels was a decelerating curve. Stepwise regression was used to develop the predictive models for the specific growth rates. Maintenance rations and optimum rations both increased with increased temperature. The relationship between maintenance, optimum rations and body weight was influenced by temperature. Conversion efficiencies increased with ration from zero at maintenance ration to a peak at the optimum ration, then declined with further increased ration. Contribution No. 3127 from the Institute of Oceanology, Chinese Academy of Sciences. Project 39370542 supported by NSFC and study also supported by National Key Laboratory in the Institute of Hydrobiology, Chinese Academy of Sciences.