泉州湾、围头湾翡翠贻贝海区半人工采苗技术研究

本文对泉州湾和围头湾翡翠贻贝的生殖腺性状、繁殖期、幼虫数量分布、附苗期及海区半人工采苗技术进行了研究。两湾翡翠贻贝繁殖期为5-9月,5月中旬至6月中旬为繁殖盛期,主要的附苗季节在6月中旬和7月上旬。选用胶胎绳和棕绳为采苗器,附苗效果较好;并讨论了投挂采苗器的适宜时间。     

围头弯悲翠贻贝海区禾苗生态

悲翠贻贝是亚热带暖水性经济贝类，近几年来人工养殖发展迅速，为了充分利用海区自然苗种资源，１９９２－１９９３年我们在围头湾进行了海区半人工采苗试验。结果是悲翠贻贝１９９３年附苗期在５月２５日至８月初，高峰斯出现在６月２０日至７月５日，采苗最佳水层在水面下１．５－３ｍ间。     

东山湾翡翠贻贝海区半人工采苗技术研究

本文研究了东山湾翡翠贻贝的繁殖期，附苗期及海区半人工采苗技术。结果表明：东山湾翡翠贻贝的繁殖期在５－１０月，其５－６月为繁殖盛期。贻贝附苗季节在６－１１月，主要附苗季节在６月上中旬至７月初。通过对东山湾７片采苗区海水理化因子的测定和采苗效果的比较试验，确定了东山湾合适的采苗区为２＾＃－６＾＃区，其中３＾＃，４＾３，５＾＃区采苗效果最好，同时筛选出了木麻黄，胶皮合股绳和泡沫编织袋等３种附苗效果较好的     

广西沿海翡翠贻贝移殖试验

1964年至1974年在广西沿海进行野生翡翠贻贝移殖试验,其结果是种贝在试养海区能够正常生长和繁殖,半人工采苗和人工育苗均获成功,幼苗下海养殖生长情况正常。28年(1967-1995年)来,每年在部分海区的固形物上面都发现有相当数量附着生长的翡翠贻贝稚贝和成贝,已在海区形成自然种群,说明广西沿海的环境条件适合翡翠贻贝的生长繁殖。广西沿海东起合浦英罗港、西至防城珍珠港、南达北海涠洲岛的河口湾外的广阔潮下带海区是适宜进行翡翠贻贝人工增、养殖的。     

莱州市海湾扇贝秋季自然海区半人工采苗初获成功

莱州湾东南岸海湾扇贝每年秋季都自行产卵。笔者在栉孔扇贝半人工采苗取得极大成功的启示下，经过４年探索，终于在１９９２年取得了海湾扇贝秋季自然海区半人工采苗的试验成功，共投放采苗袋１００个，采苗２０×１０４粒左右。１９９２年１１月２８日特邀请中科院海洋研究所张福绥研究员首次到莱州指导工作，经检查每袋采苗量２１００多粒，壳高２～３ｍｍ。目前笔者正进一步研究海湾扇贝秋苗的越冬问题和在生产中的应用价值。莱州市海湾扇贝秋季自然海区半人工采苗初获成功@李学祥@周经济     

栉孔扇贝秋季半人工采苗技术

随着扇贝养殖业的发展,扇贝人工育苗技术和春季半人工采苗技术都有了很大改进和提高。但对栉孔扇贝的秋季半人工采苗技术却很少研究。为解决苗源不足问题,1986年我们在荣成桑沟湾海区进行了栉孔扇贝秋季半人工采苗试验,并取得了显著效果。     

胶州湾贻贝苗源的发展

贻贝(Mytilus edulis L.)是一种冷水性双壳类软体动物，它在我国沿岸分布的南限是胶州湾，但仅见于港内码头及船底上，且数量不多。 1958年青岛市水产局、原青岛市海水养殖场和中国科学院海洋研究所等单位曾一起自大连和烟台移贻贝苗至青岛试养，次年养殖架上也曾有少量幼苗附着，但未能大量繁殖起来。1971年后胶南县海水养殖试验场及青岛市第二海水养殖场等先后进行数十亩乃至数百亩移苗养殖，附苗量虽有所增长，但直至1974年仍缺乏生产意义。这种情况引起我们的思考:胶州湾贻贝苗源能否大量发展？它的限制因素是什么？ 1972年至1973年，我们与烟台地区海水养殖试验场等单位曾对烟台沿岸贻贝苗源进行调查研究，确认当时烟台苗种产量不高的主要原因是附着基不足，其次是亲贝不足，据此试验成功了“废旧草绠采苗法”。之后，随着采苗器材的增加及养殖面积的不断扩大，烟台芝罘湾已发展成为一个较好的苗场，通常可供万亩以上生产用苗。这个经验对我们开发胶州湾的苗源很有启发。 1975年胶南县在胶州湾大力推广“废旧草绠采苗法”，收割海带后保留废旧海带架两万台采贻贝苗， 1976年进一步扩大贻贝养殖面积，继续留绠采苗。我们在青岛市水产局及胶南县水产局等单位的协助下，于1975年9月及1976年8-9月，对胶州湾内及湾口(青岛前海)留绠采苗的情况进行了调查，这些调查资料即为本文的主要依据。     

栉孔扇贝自然海区采苗技术研究

1974-1985年在烟台市、荣成和长岛县进行了自然海区采苗的试验研究。研究结果表明，采苗袋(笼)是较好的扇贝自然海区采苗器。自然海区采苗技术的关键是：采苗袋网目在1．5毫米左右，内放少量网片作为稚贝附着基；海区透明度大；适时预报投放采苗器时间；掌握适宜的采苗水层。该项技术经济效益显著。     

树脂纸板在牡蛎附苗与养成上的应用

１９８８～１９８９年，作者在山东省海阳县进行树脂涂料器材的采苗和养成筛选试验，取得了一定的效果。１９９０年，将海区试验移到了浙江省乐清湾进行，经过棚架式采苗和浮筏式养殖，附苗密度、生长速度，都能达到生产要求。１９９０年９月４日，对采苗情况进行验收，得到了专家及同行的好评。１９９１年扩大试验到１０００片，与橡皮条交叉分挂在同一棚架上采苗，然后移到同一浮筏上养成。养成期间，几次抽样检测，分别测量其密度和大小。１９９２年４月９日，委托专家和同行再次验收，以检测的结果，推算出一台筏架树脂纸板和一台筏架橡…     

同池中垂动苗帘与静置苗帘贻贝采苗效果的比较

在同一贻贝育苗池中进行了垂动苗帘与静置苗帘采苗效果的比较实验;采苗后又将两种苗帘移至同一海区养育,比较其保苗效果。实验结果表明,苗帘活动能够明显提高采苗数量以及附苗的均匀程度,但对海上的保苗效果无甚倾向性影响。     

Microbial Biomass and Organic Nutrients in the Deep-Sea Sediments of the Central Indian Ocean Basin

In order to assess the impact of deep-sea mining on the in situ benthic life, we measured the microbial standing stock and concentration of organic nutrients in the deep-sea sediments of the Central Indian Ocean Basin in the Indian pioneer area. Sediments were collected using box core and grab samples during September 1996. The total bacterial numbers ranged from 10 10 -10 11 cells per g -1 dry weight sediment. There was a marginal decrease in the number of bacteria from surface to 30 cm depth, though the subsurface section registered a higher number than did the surface. The highest numbers were encountered at depths of 4-8 cm. The retrievable number of bacteria were two orders less in comparison with the direct total counts of bacteria. An almost homogeneous distribution of bacteria, total organic carbon, living biomass, and lipids throughout the depth of cores indicates active microbial and benthic processes in the deep sea sediments. On the other hand, a uniform distribution of total counts of bacteria, carbohydrates, and total organic carbon in all the cores indicates their stable nature and suggests that they can serve as useful parameters for long-term monitoring of the area after the benthic disturbance. Further studies on temporal variability in this region would not only verify the observed norms of distribution of these variables but would also help to understand restabilization processes after the simulated benthic disturbance.     

Assessing the Validity of Seismo-Acoustic Predictor Equations for Obtaining Seabed and Sub-Surface Sediment Physical Properties

The interdependence between the seismo-acoustic properties of a marine sediment and its geotechnical/physical parameters has been known for many years, and it has been postulated that this should allow the extraction of geotechnical information from seismic data. Though in the literature many correlations have been published for the surficial layer, there is a lack of information for greater sediment depths. In this article, a desktop study on a synthetic seafloor model illustrates how the application of published near-surface prediction equations to subsurface sediments (up to several tens of meters burial depth) can lead to spurious predictions. To test this further, acoustic and geotechnical properties were measured on a number of sediment core samples, some of which were subjected to loading in acoustically-equipped consolidation cells (oedometers) to simulate greater burial depth conditions. For low effective pressures (representing small burial depths extending to around 10 meters subsurface), the general applicability of established relationships was confirmed: the prediction of porosity, bulk density, and mean grain size from acoustic velocity and impedance appears generally possible for the investigated sedimentary environments. As effective pressure increases through, the observed relationships deviate more and more from the established ones for the near-surface area. For the samples tested in this study, in some instances increasing pressure even resulted in decreasing velocities. There are several possible explanations for this abnormal behavior, including the presence of gas, overconsolidation, or bimodal grain size distribution. The results indicate that an appropriate depth correction must be introduced into the published prediction equations in order to obtain reliable estimates of physical sediment properties for greater subsurface depths.     

Geoacoustic Inversion via Genetic Algorithm and Its Application to Manganese Sediment Identification

An acoustic inversion method using a wide-band signal and two near field receivers is proposed and applied to multiple layered seabed models including a manganese sediment. The inversion problem can be formulated into a probabilistic model comprised of signals, a forward model, and additive noise. The forward model simulates wide-band signals, such as chirp signals, and is chosen to be the source-waveletconvolution plane wave modeling method. The wavelet matching technique, using weighted least-squares fitting, estimates the sediment sound-speed and thickness on which determination of the possible numerical ranges for a priori uniform distribution is based. The genetic algorithm is applied to a global optimization problem to find a maximum a posteriori solution for determined a priori search space. Here the object function is defined by an L 2 norm of the difference between measured and modeled signals. Not only the marginal pdf but also its statistics are calculated by numerical evaluation of integrals using the samples selected during importance sampling process of the genetic algorithm.     

海上大直径钢管桩打桩过程中桩周土强度弱化研究

海上大直径钢管桩打桩过程中,桩周土体受到强烈扰动而发生强度弱化,掌握桩周土体强度弱化规律对于准确预测打桩过程、保证工程安全具有重要意义。为研究土体强度弱化规律,开展了环剪试验模拟打桩对桩周土体的扰动,测试土体强度随剪切速率的变化规律,建立了描述土体强度弱化规律的拟合公式,引入到打桩分析软件中。研究结果表明：土体的强度折减程度不仅与土体本身的性质有关还受到土体的埋深和剪切速率的影响,埋深越深土体强度折减程度越低,剪切速率越高土体强度折减越高,在打桩分析中可采用这里推荐的线性折减方法来模拟不同深度处土体强度的折减规律。     

Interannual Sea Level Variations and Annual Tides in the Northwestern Pacific

Specific properties of the interannual sea level variations and annual tides in the Northwestern Pacific were studied. Several tide stations were monitored. The monthly mean sea level for the year of 1995 was analyzed at each tide station. A seismic event in 1995, some tectonic activity around the subject area, and the Kuroshio (the oceanic western boundary current) may possibly contaminate results which would have occurred from the astronomical annual tide alone.     

Deep-Sea Sediment Compression Curves: Some Controlling Factors, Spurious Overconsolidation, Predictions, and Geophysical Reproduction

Oedometer tests have been carried out on 70 undisturbed surficial clays (at approximately 250 mm below the mudline), mostly collected by free-fall corers from sites widely scattered throughout the deep-sea North Atlantic. Acoustic measurements were also made, initially on contiguous samples and ultimately on the same sample using a geophysically instrumented oedometer which also collected electrical resistivity data. Apart from those quiescent areas below the carbonate compensation depth, such as north of the West Indies where very fine clays exist, most of the samples are silty clays whose geotechnical-geophysical properties are dependent on the type of clay minerals present (and their ability to take in moisture), the sand-size fraction, and the quantity of carbonate present. Thus the pure clays have high compressibilities which decrease on the addition of coarse particles, while the converse is true for the acoustic parameters, these increasing with the sand fraction. Using the notion of the intrinsic compression line for all samples, and comparison to it of the measured compression curves, it is clear that, contrary to some previously held ideas, most deep-sea clays are normally consolidated; the addition of carbonate has the effect of creating an open, stronger sediment skeleton. Interestingly, where information is available, the variation with depth of a sample's acoustic velocity follows the void ratio pressure relationship of the compression curve. This allows the construction of an in-situ sediment compression curve using the in-situ geophysical observations.     

Flow Cytometric Analysis of Coastal Lagoon Bacterioplankton and Picophytoplankton: Fixation and Storage Effects

The effect of different fixation and storage protocols on the flow cytometric (FCM) simultaneous analysis of bacterioplankton and phytoplankton in coastal seawater samples (Mediterranean coastal lagoons) was investigated. FCM measurements (cell number, fluorescence and scatter characteristics) were obtained through DAPI staining. Three fixatives [glutaraldehyde (GA), formaldehyde (FA) and paraformaldehyde (PFA)] and two storage (3 months duration) methods (5 °C and −196 °C) were tested. Two dominant populations were detected in studied samples: bacteria and eukaryotic picophytoplankton. Adding fixatives (2% final concentration) appears necessary to obtain FCM exhaustive counts of all the bacteria and phytoplanktonic cells. This was related to the permeation effect of fixatives which allowed a better DAPI staining of the cells. Maximum fluorescence, i.e. optimal staining of the cells was obtained with FA or PFA, and significant lower fluorescences with GA. Fixed samples stored at 5 °C induced rapid cell loss. Only storage in liquid nitrogen of samples fixed with FA or PFA, allows mid-term (≥4 months) preservation of bacteria or picophytoplankton cell numbers, and limited evolution of DAPI-induced fluorescence and scatter characteristics.     

Phylogenetic diversity and phenotypic characterization of cultivable bacterioplankton isolated from polar oceans

A set of 27 marine planktonic bacteria isolated from the polar regions was characterized by 16S rDNA sequencing and physiological and biochemical testing. More than half of these bacteria were positive for caseinase, gelatinase and 13-glucosidase, and could utilize glucose, maltose or malic acid as carbon source for cell growth. Twelve isolates expressed nitrate reduction activities. Except for one antarctic isolate BSwlO175 belonging to Actinobacteria phylum, these isolates were classified as γ-Proteobacteria, suggesting that γ-Proteobacteria dominated in cultivable marine bacterioplankton at both poles. Genus Pseudoalteromonas was the predominant group in the Chukchi Sea and the Bering Sea, and genus ShewaneUa dominated in cultivable bacterioplankton in the Prydz Bay. With sequence similarities above 97%, genus Psychrobacter was found at both poles. These 27 isolates were psychrotolerant, and significant 16S rDNA sequence similarities were found not only between arctic and antarctic marine bacteria （ 〉 99% ）, but also between polar marine bacteria and bacteria from other aquatic environments （ ≥ 98.8% ）, including temperate ocean, deep sea, pond and lake, suggesting that in the polar oceans less temperature-sensitive bacteria may be cosmopolitan and have a bipolar, even global, distribution at the species level.