Cloning and characterization of glyceraldehyde-3-phosphate dehydrogenase encoding gene inGracilaria/Gracilariopsis lemaneiformis

Glyceraldehyde-3-phosphate dehydrogenase （GAPDH） plays important roles in various cellular processes. A cytosolic GAPDH encoding gene （gpd） of Gracilaria/Gracilariopsis lemaneiformis was cloned and characterized. Deduced amino acid sequence of the enzyme of G. lemaneiformis had high homology with those of seven red algae. The 5＇-untranslated regions of the GAPDHs encoding genes of these red algae varied greatly. GAPDHs of these red algae shared the highly conserved glyceraldehyde 3-phosphate dehydrogenase active site ASCTTNCL. However, such active site of Cyanidium caldarium was different from those of the other six algae at the last two residues （CL to LF）, thus the spatial structure of its GAPDH active center may be different from those of the other six. Phylogenetic analysis indicated that GAPDH of G. lemaneiformis might have undergone an evolution similar to those of Porphyra yezoensis, Chondrus crispus, and Gracilaria verrucosa. C. caldarium had a closer evolutionary relationship with Cyanidioschyzon merolae than with Cyanidium sp. Virtual Northern blot analysis revealed that gpd of G. lemaneiformis expressed constitutively, which suggested that it might be house-keeping and could be adapted as an inner control in gene expression analysis of G. lemaneiformis.     

Post-depositional modification of NO-3 in snow layers at EastAntarctica and at the headwater of ürumqi River

Acidic species, such as Nitrate, in polar snow and firn layers are “reversibly” deposited, and are sufficiently volatile to undergo significant postdepositional exchange between snow/firn and the atmosphere. Through comparison of the snowpit and snowpack nitrate concentrations from central East Antarctica and the headwater of ürumqi River, we conclude that the nitrate peaks in the uppermost surface snow layers in central Antarctica are not related to an atmospheric signal and must account for post-depositional effects. Such effects, however, are not found in the surface snowpack nitrate profiles from the headwater of ürumqi River. Two reasons may account for the post-depositional difference. At first, nitrate in the polar snow and firn layers appears to be hydrated ion, which can be taken up by the atmosphere, while at the headwater of ürumqi River it seems mainly as mineral ion, which assembles the behavior of aerosol-derived species that are “irreversibly” deposited and do not undergo significant post-depositional exchange with the atmosphere. Secondly, the chemical features of the snow and ice on the Antarctica are mainly determined by wet deposition, to the contrary, dry deposition is more significant at the headwater of lUrumqi River than that on the East Antarctic Plateau.     

长江口斜压诊断模式三维流场数值模拟

建立了σ坐标系下长江口斜压诊断模式三维流场数学模型,采用k-kl二方程紊流闭合模型求解垂向涡粘系数,计算域内恒定、非均匀盐度场反映了计算域密度斜压效应。验证结果表明,斜压诊断模式的模拟成果较切合实际。模型成功复演了长江口水域由往复流向旋转流过渡的流场特性;潮波从四条汊道传入后在分汊口附近相遇形成多个会潮点,其位置随着径流的大小、潮汐的强弱、汊道的形态等因素而变化。     

NCAR绘图软件在Windows下的编程

介绍了NCAR绘图软件在Windows下的编程方法和具体实现，解决了在科研绘图工作中遇到的一些实际问题，对在Windows下编程的静态链接和动态链接进行了分析和研究，为Fortran编程员提供了在Windows下编程的基础。     

雷电临近预警产品评估方法及其软件

为了适应雷电业务发展的需求,对已经投入业务运行试验的雷电临近预警系统的预警效果做出客观评估,中国气象科学研究院雷电物理和防护工程实验室开展了雷电临近预警产品评估方法的研究,并开发了配套的应用软件.评估方法通过雷电预警结果和实际监测结果的对比,得到命中率POD、虚警率FAR和TS评分三项指标,实现对雷电预警产品的评估.评估软件的实际运行表明:该方法不仅能够检验CAMS_LNWS预报雷电活动的能力,还能对预警方法的改进起到一定的指导作用,能够满足雷电业务产品评估的需要.     

涡北河网区三维地震采集技术

涡北勘探区属于河网地带,难以进行正常的地震施工。为此,本文提出了一种以规则束状观测为基础和特殊观测系统相结合的数据采集方法。该方法适应了这种复杂的地表条件,获得了"空白区"的地震资料,为今后类似地表条件地区的三维地震勘探提供了经验。      

四川九寨黄龙机场高填方地基变形三维动力分析

四川九寨黄龙机场具有高填方、高地震烈度、高海拔等三高的特点,高填方体地基在地震作用下的动力稳定与变形问题成为该机场需着重考虑的主要工程地质问题之一。在对目前各种动力分析方法研究的基础上,选用快速拉格郎日差分分析(FLAC)程序,对该机场元山子沟段高填方地基在地震作用下的变形规律进行了系统地研究,获得了一些有实际意义的认识。     

Directional dependence of P- and S-wave propagation and polarization in foliated rocks from the Kola superdeep well: Evidence from laboratory measurements and calculations based on TOF neutron diffraction

We have measured P- and S-wave velocities on two amphibolite and two gneiss samples from the Kola superdeep borehole as a function of pressure (up to 600 MPa) and temperature (up to 600 °C). The velocity measurements include compressional (Vp) and shear wave velocities (Vs1, Vs2) propagating in three orthogonal directions which were in general not parallel to inherent rock symmetry axes or planes. The measurements are accompanied by 3D-velocities calculations based on lattice preferred orientation (LPO) obtained by TOF (Time Of Flight) neutron diffraction analysis which allows the investigation of bulk volumes up to several cubic centimetres due to the high penetration depth of neutrons. The LPO-based numerical velocity calculations give important information on the different contribution of the various rock-forming minerals to bulk elastic anisotropy and on the relations of seismic anisotropy, shear wave splitting, and shear wave polarization to the structural reference frame (foliation and lineation). Comparison with measured velocities obtained for the three propagation directions that were not in accordance with the structural frame of the rocks (foliation and lineation) demonstrate that for shear waves propagating through anisotropic rocks the vibration directions are as important as the propagation directions. The study demonstrates that proper measurement of shear wave splitting by means of two orthogonal polarized sending and receiving shear wave transducers is only possible when their propagation and polarization directions are parallel and normal to foliation and lineation, respectively.     

一个简易的CRM系统的设计与实现--汽修连锁资源管理系统

首先提出引入CRM和连锁经营的必要性和紧迫性,并分析了国内外现状,接着对客户关系管理和连锁经营的基本概念及其相关理论进行阐述,最后具体实现这个系统,包括硬件架构和软件模型.     

东北地区大气污染物源排放时空特征：基于国内外清单的对比分析

基于国内外4类常用的污染源排放清单数据(EDGAR:全球大气研究排放数据库;CEDS:社区排放数据系统;MIX:亚洲排放清单;PKU-FUEL:全球燃料排放数据),对东北区域5类人为排放源(工业源、能源、交通运输源、生活民用源和农业源)的8种污染物(PM_2.5、PM₁₀、SO₂、NO_x、NMVOCs、NH₃、OC和BC)从排放总量、来源贡献和时空分布特征等方面进行对比分析。结果表明:东北污染物排放主要以SO₂、NO_x和NMVOCs为主,工业、能源和交通运输为主要贡献源;PM_2.5和PM₁₀主要来自生活民用源和工业源,贡献率前者大于后者。辽宁省污染物(除NH₃外)排放最大,其次为黑龙江省、吉林省和内蒙古东四盟市,冬季排放强度明显高于其他季节。NH₃主要来自农业源,排放峰值发生在5～7月;各清单间排放总量和来源贡献差异明显,EDGAR和PKU清单对NH...