The Effect of the Introduction of the American Species Marenzelleria viridis (Polychaeta; Spionidae) on the Benthic Ecosystem of Vistula Lagoon

Abstract. In the late 1980s, a radical change in the macrozoobenthos composition occurred in the Vistula Lagoon, an oligohaline and silted water basin. This structural change is a result of the mass appearance of the very strongly euryhaline spionid polychaete Marenzelleria viridis Verrill, a newcomer from North American estuaries. It considerably reduced the previous bottom macrofauna, which was composed mainly of Chironornidae larvae and oligochaetes.
The penetration depth of the macrozoobenthos into the sediments and its total biomass increased in the last years, which enhanced the organic matter displacement in sediment layers of a greater thickness. The previous bottom dwellers inhabited mainly the most superficial sediment layer, whereas the new polychaete immigrant can penetrate down to a depth of 20–40 cm.     

Effect of size, tissue parts and location on six biochemical markers in the green-lipped mussel, Perna viridis

The effect of size and tissue parts as well as site locations on a suite of six anti-oxidative biochemical markers, viz. the enzyme degrading neural transmitters acetylcholinesterase (AChE), the Phase II transforming enzyme glutathione-S-transferase (GST), the anti-oxidative enzymes superoxide dismutase (SOD) and catalase (CAT), the lipid metabolite malondialdehyde (MDA) and the ratio of CAT/MDA, in the bivalve mollusc, Perna viridis, was studied. Field mussels were collected from the piers of University of Science and Technology (UST), Pak Sha Wan or Hebe Haven (PS) and Sai Kung (SK) in Port Shelter, Hong Kong. Statistically, ANOVA showed that the activity of all six biochemical markers were generally tissue dependent, size independent and location dependent. Tukey multi comparison test indicated that the biochemical activities fell into two groups, with SK the highest activity group in most cases. The significance of these findings in relation to long-term coastal water biomonitoring is discussed.     

3种海洋微藻微胶囊培养的初步研究

用12mg/mL羧甲基纤维素钠(CMC)、10mg/mL海藻酸钠(SA)和0.5mmol/mL CaCl2制备亚心形扁藻、绿色杜氏藻和球等鞭金藻8701的中空海藻酸钙微胶囊,3种微藻在微胶囊内生长表现突出,最高细胞密度亚心形扁藻为70.4×106/mL,绿色杜氏藻为100.9×106/mL,球等鞭金藻8701为264.1×106/mL,分别是悬浮培养微藻最高密度的35倍、27倍和84倍,结果说明微胶囊化培养是微藻高密度培养的有效方式。     

基于COI序列的翡翠股贻贝Perna viridis线粒体遗传特性分析及其近缘种间的系统关系探讨

应用通用引物 COIL 1490和 COIH 2198对翡翠股贻贝Perna viridis的性腺和体细胞线粒体DNA进行PCR扩增，获得661bp长度的COI基因片段，经过比对性腺与体细胞的COI片段，发现雄性性腺与体细胞COI基因均为一个单倍型，即体内只有一种线粒体DNA类型，没有发现双单性遗传现象，雌、雄性腺的COI基因片段变异率很低（0.31%）。应用PAUP构建了NJ树、MP树以及贝叶斯法构建了贝叶斯树，对股贻贝属3种间的系统关系进行了分析，结果表明，翡翠股贻贝P. viridis与P. canaliculus 和 P. perna 之间的分化与分歧年代的估算是相吻合的。     

4种海洋贝类多糖提取和保湿性研究

以马氏珠母贝(Pinctada martensii)、企鹅珍珠贝(Pteria penguin)、翡翠贻贝(Perna viridis)和近江牡蛎(oyster)的内脏团为原料提取、纯化多糖,与甘油、聚乙二醇400(PEG-400)、丙二醇和1,3-丁二醇四种常规保湿剂进行比较.结果表明,在相对湿度为44%时,前12h 吸湿率顺序为:马氏珠母贝<近江牡蛎<企鹅珍珠贝<翡翠贻贝< PEG-400<1,3-丁二醇<甘油<丙二醇;在相对湿度为80%时,前12 h 吸湿率顺序为:近江牡蛎<马氏珠母贝<企鹅珍珠贝<1,3-丁二醇<翡翠贻贝< PEG-400<甘油<丙二醇.前12 h 的保湿率顺序为:近江牡蛎>翡翠贻贝>马氏珠母贝>企鹅珍珠贝>甘油>1,3-丁二醇>丙二醇> PEG-400.翡翠贻贝吸湿性多糖优于其他贝类多糖,稍低于常规保湿剂.在相对湿度为80%时,前12 h 翡翠贻贝多糖的吸湿率大于常规保湿剂1,3-丁二醇,翡翠贻贝多糖保湿性稍弱于近江牡蛎,但强于其他贝类多糖和常规保湿剂要强一些     

The Influence of Metoclopramide and Melatonin on Activity and Schooling Behaviour in Chromis viridis (CUVIER, 1830; Pomacentridae, Teleostei)

Abstract. The role of prolactin (PRL) in the neuroendocrine control of schooling behaviour and activity in the demoiselle Chromis viridis is analysed by administration of a prolactin-releasing drug, metoclopramide (Paspertin®). In addition, the dependence of the activity state, schooling time, and colour changes on the administration of an epiphysial hormone, melatonin, is examined. Each substance tested affects schooling behaviour and activity by a different mechanism.     

翡翠贻贝足丝器的组织结构观察

用光镜和电镜技术初步探讨了翡翠贻贝Ｐｅｒｎａｖｉｒｉｄｉｓ足丝器（Ｂｙｓｓａｌａｐｐｒａｔｕｓ）的三大组成部分之间的空间结构关系，着重观察足丝柄部发生器（ＢＳＧ）的片层结构，揭示小片具有典型的胶原原纤维电镜特征。此外，通过足丝丝部的表面结构观察，发现可用众多的突起来解释足丝表面对放射性碘的强机械吸附作用；利用足丝丝部横切片，计算出其长径约１０５μｍ，短径约４０μｍ。     

广西沿海翡翠贻贝移殖试验

1964年至1974年在广西沿海进行野生翡翠贻贝移殖试验,其结果是种贝在试养海区能够正常生长和繁殖,半人工采苗和人工育苗均获成功,幼苗下海养殖生长情况正常。28年(1967-1995年)来,每年在部分海区的固形物上面都发现有相当数量附着生长的翡翠贻贝稚贝和成贝,已在海区形成自然种群,说明广西沿海的环境条件适合翡翠贻贝的生长繁殖。广西沿海东起合浦英罗港、西至防城珍珠港、南达北海涠洲岛的河口湾外的广阔潮下带海区是适宜进行翡翠贻贝人工增、养殖的。     

翡翠贻贝鳃的组织形态学和超微结构观察

应用光镜和透射电镜观察翡翠贻贝鳃的组织形态学和超微结构．翡翠贻贝身体两侧各有一对鳃瓣，覆盖在内脏团之上，鳃瓣由众多鳃丝组成．与其它种类贻贝相似，翡翠贻贝鳃丝上皮可区分为前微绒毛柱状细胞、前侧纤毛柱状细胞、立方或扁平细胞和后微绒毛柱状细胞等4个不同区带．电镜水平下可观察到鳃丝上具微绒毛柱状细胞、具纤毛柱状细胞、立方或扁平细胞、间质细胞和粘液细胞等5种细胞类型．本文对鳃丝的组织形态学及各类细胞的超微结构与功能作了描述及讨论．