基于COI序列的翡翠股贻贝Perna viridis线粒体遗传特性分析及其近缘种间的系统关系探讨

应用通用引物 COIL 1490和 COIH 2198对翡翠股贻贝Perna viridis的性腺和体细胞线粒体DNA进行PCR扩增，获得661bp长度的COI基因片段，经过比对性腺与体细胞的COI片段，发现雄性性腺与体细胞COI基因均为一个单倍型，即体内只有一种线粒体DNA类型，没有发现双单性遗传现象，雌、雄性腺的COI基因片段变异率很低（0.31%）。应用PAUP构建了NJ树、MP树以及贝叶斯法构建了贝叶斯树，对股贻贝属3种间的系统关系进行了分析，结果表明，翡翠股贻贝P. viridis与P. canaliculus 和 P. perna 之间的分化与分歧年代的估算是相吻合的。

Genetic analysis of the marine mussel Perna viridis (Bivalvia: Mytilidae) based on mitochondrial COI sequence and its phylogenetic relationship with other congeneric species

Partial mitochondrial COI gene sequences from the gonads (male and female) and somatic tissue of mussel Perna viridis were separately amplified by PCR using the universal primers COIL 1490/COIH 2198. A 661 bp nucleotide sequence of COI gene was obtained. It was found that this gene sequence was identical among different tissues of male individual, and low divergence (0.31%) of COI sequence was detected between male and female of the gonad tissues. Only one haplotype was found in both female and male for the sequence. No doubly uniparental inheritance (DUI) was detected. Based on the COI sequences obtained from this study and those of other Perna species from GenBank, NJ tree, MP tree and Bayesian inference (BI) tree were constructed with PAUP and MrBayes respectively, and phylogenetic relationships among them were analyzed accordingly.