EFFECT OF LIGHT INTENSITY ON THE TOTAL LIPID AND FATTY ACID COMPOSITION OF SIX STRAINS OF MARINE DIATOMS

The effect of light intensity ( 1500 Ix and 5000 Ix) on the total lipid and fatty acid composition of six strains of marine diatoms Cylindrotheca fusiformis (B211 ), Phaeodactylum tricornutum (Bl14, Bl18 and B221) Nitzschia closterium (B222) and Chaetoceros gracilis (B13) was investigated.The total lipids of B13, Bl14, and B211 grown at 5000 Ix were lower than those grown at 1500 Ix. No evident changes were observed in Bl18, B221 and B222. Fatty acid composition changed considerably at different light intensity although no consistent correlation between the relative proportion of a single FA and light intensity. The major fatty acids of the 6 strains were 14:0, 16:0, 16:l(n- 7) and 20:5(n-3) . Cylindrothecafusiformis had high percentage of 20:4n - 6 (9.2 - 10.9% ) . The total polyunsaturated fatty acid /rl all 6 strains decreased with increasing light intensity. The percentage of the highly unsaturated fatty acid eicosapentaenoic acid (EPA) decreased with increasing light intensity in all strains except Chaetoceros gracilis.     

Seasonal and Spatial Variations of Total Mass Flux around Coral Reefs in the Southern Ryukyus,Japan

Total mass flux, size distribution of sediment particles and some chemical components such as total carbon (TC), total nitrogen (TN) and calcium carbonate (CaCO₃) were monitored monthly using a multi-cup sediment traps at seven coral reef sites (6 reef flat and 1 reef slope) of the Marine Protected Areas around Ishigaki, Kohama, Kuroshima and Iriomote Islands in the southern Ryukyus, Japan from September 2000 to September 2001. The size distribution of trapped sediments revealed mostly uni-modal fine sand to mud in the reef flat and gravelly to coarse sand in the reef slope. The total mass flux ranged between 0.54 to 872 gm⁻²d⁻¹, and showed a pronounced seasonality (high in summer-autumn and low in spring) at each site, which was consistent with the rainfall and typhoon regime. Exceptionally high values were observed on the reef slope (Iriomote) in February–March 2001 (1533 gm⁻²d⁻¹) owing to a large amount of bottom sediment re-suspension. On the reef flat (Todoroki South and North; Ishigaki), values obtained in July–August 2001 (872 gm⁻²d⁻¹) and August–September 2001 (800 gm^{− 2}d⁻¹) indicate the high terrestrial discharge from Todoroki River. Trapped sediment particles consist of CaCO₃ (1.2–27.1%) and a non-carbonate fraction (98.8–72.9%), which contains total carbon (4.9–26%), carbonate carbon (CO₂-C) (0.2–3.1%) and non-carbonate carbon (NC-C) (7.9–25.6%). Total nitrogen content was in the range 0.02–0.48%. TN is contained mainly in the carbonate fraction and NC-C may be contained in the non-carbonate fraction. The low TN/OC ratio of the trapped sediments suggests that they were mostly of terrestrial origin and that both fractions migrated. The high total mass flux derived from Todoroki River exceeded the threshold at which a lethal effect on coral community is caused. The results stress the importance of conducting seasonal studies of sedimentation over more than one year and at more than one location in south Japan coral reef ecosystems to gain an understanding of the processes controlling the total mass fluxes and their nutrients content, also to develop an awareness of how to prevent the damage of coral reef ecosystems and, if it does occur, to allow mitigation measures to be undertaken.     

Total Lipid and Fatty Acid Classes in Decomposing Mangrove Leaves of <Emphasis Type="Italic">Bruguiera gymnorrhiza</Emphasis> and <Emphasis Type="Italic">Kandelia candel</Emphasis>: Significance with respect to Lipid Input

Changes in the concentration of total lipid and fatty acids (FAs) during the decomposition of mangrove leaves were investigated by field experiments using yellow leaves of Bruguiera gymnorrhiza (L.) Lamk. and Kandelia candel (L.) Druce, in order to quantify mangrove contribution to lipid and fatty acid inputs to marine sediments. Total lipid and total FA in the fresh (green and yellow) and decomposing leaves of both species were significantly higher during winter than summer. During decomposition, total lipid content and FA concentration, in particular branched chain fatty acids (BrFAs) and bacterial fatty acids (BFAs), increased to a maximum concentration in 45 days during winter and in 17 days during summer. Lipids were lost faster in K. candel leaf detritus than in B. gymnorrhiza leaf detritus in which >90% of the total lipid original weight was lost during the summer experiment and <60% during the winter experiment. The changes in the concentrations of total lipids and FAs in the decomposing leaves also indicate that mangrove leaves are significant sources of fatty acids and probably other lipid compounds to estuarine ecosystems and that tidal waters transport the lipids and FAs adsorbed to particulate matter from mangroves to adjacent estuarine sediments and the ocean.     

台湾海峡中北部上升流区各种形态磷的化学特性

根据１９８８年７月调查资料讨论了台湾海峡中北部海域夏季上升流区各种形态磷的分布特征。结果表明，上升流中心区（即海坛岛东侧一带水域）具有低温、高盐、低溶解氧；ＤＩｐ，ＴＤＰ，ＰＰ，ＴＰ较高；ＤＯＰ较低（０．２７）；及ＤＩＰ／ＤＯＰ比值（约为１）较高的特性。ＤＯＰ是该海域磷的主要形态（占ＴＰ的４９．２％），其含量分布及其形态转化与生物活动直接相关，是该海域生物生产力高的体现。     

pH对眼点拟微绿球藻的生长、总脂含量及脂肪酸组成的影响

pH对眼点拟微绿球藻（Namnocholoropsis oculata）的生长、总脂含量及脂肪酸组成有明显影响。研究表明，眼点拟微绿球藻的生长对pH有较强的适应性，在pH6.2～9.8的范围内均能较好的生长，但在pH5.5的条件下生长受到较强的抑制，在不同pH条件下，在同一细胞周期内总脂含量及脂肪酸组成均有相同的变化规律，即总脂含量在对数初期略有下降然后逐渐上升，在稳定期达到最大值。16：0，18：1n9的含量在稳定期显著升高，而EPA的含量在稳定期明显下降，在对数初期，藻细胞在pH6.8的条件下含有较高的EPA含量，而在pH9.8的条件下，藻细胞的EPA含量明显降低。     

2001-2002年天目湖(沙河水库)浮游植物的生态学研究

2001年6月~2002年5月,对天目湖进行的浮游植物周年调查表明,天目湖共有浮游植物7门75属,其中年平均丰度为5026.20×104个/L,以蓝藻为主,占总丰度的54.45%;而年平均生物量为(15.364±9.103)mg/L,其中硅藻居首位,为6.634 mg/L,占浮游植物年平均生物量的43.18%;其周年变化是丰度和生物量最高值均出现在5月,丰度在5月和9月出现2个峰值,而生物量则在5、7、10月出现3个峰值。浮游植物丰度从大坝处1#点到河流入湖口的9#、10#点变化不明显但略有增加,而生物量则呈明显增加。通过对水温、透明度、营养盐与浮游植物丰度和生物量的线性回归分析发现,浮游植物丰度、生物量与水温、TP存在显著的正相关,而与N/P比、透明度存在显著性负相关,与TN相关性不是很明显。     

光照强度对海洋微藻脂肪含量及脂肪酸组成影响的研究

近年来，海洋微藻脂肪酸组成的研究及其应用越来越受到国内外科学家的重视。高度不饱和脂肪酸(PUFA)，特别是长链的n-3 PUFA，如二十碳五烯酸(EPA)和二十二碳六烯酸(DHA)对海洋动物和人类都具有营养学和医学上的价值。在海洋微藻的培养过程中，光照强度是海洋微藻生长的条件之一，光线的明与暗，光度的强与弱，不仅对微藻的生长速率、产量有影响，而且对其脂肪含量和脂肪酸的组成也有影响(Teshima et al.，1983；Thompson et al.，1990； Renaud et al., 1991)。 作者在以往研究的基础上，选择了3种有代表性的海洋微藻：(1)小球藻Chlorella sp-2(李荷芳等，1999)，此藻脂肪酸中EPA含量高、且不含DHA；(2)球等鞭金藻(Isochrysis galbana)，该藻DHA含量较高，但几乎不含EPA；(3)前沟藻(Amphidinium sp.)的EPA、DHA含量均高。将以上3种微藻作为原料，在不同的光照强度下进行培养，测定并分析藻体中的脂肪含量和脂肪酸组成的变化，从而了解光强对海洋微藻脂肪含量及其脂肪酸组成的影响。     

卤虫无节幼体的营养强化对黑斑口虾蛄(Oratosquilla kempi)幼体发育的影响

用扁藻、酵母、“鱼油 酵母”强化和“不强化”4种方式处理的卤虫无节幼体投喂黑斑口虾蛄各期幼体,比较不同强化方法对黑斑口虾蛄幼体成活率、变态率、生长速度及总脂与脂肪酸组成的影响。结果表明,3种方式强化12h后的卤虫无节幼体与对照组相比,总脂含量都有不同程度的增加。卤虫无节幼体的脂肪酸组成与强化方式密切相关,其中“鱼油 酵母”强化的卤虫无节幼体20∶5n-3(EPA)和22∶6n-3(DHA)占总脂肪酸的比例最高,分别为5·74%和4·84%。投喂强化后富含EPA和DHA的卤虫无节幼体,可增加黑斑口虾蛄幼体体内脂肪酸尤其是EPA和DHA等不饱和脂肪酸的含量,从而提高其幼体的成活率、变态率与发育速度。     

水中总氮的紫外分光光度分析法

本文采取K_2 S_2O_8氧化法,研究了氧化条件、紫外分光光度法测定总氮的最佳条件以及可能产生的干扰及消除方法。利用本法测定天然水中总氮回收率100±2％,检出限为0.010mg·dm~(-3)。方法快速准确,与Kjedahl法相比可提高工效27倍。     

十四株海洋微藻脂肪酸组成的研究

对 4个门的 14株 (红藻门 8株 ,甲藻门 1株 ,隐藻门 2株 ,绿藻门 3株 )海洋微藻进行了脂肪酸含量测定 ,微藻在确定的条件下生长 ,指数生长末期收获。结果表明 ,各门的微藻都有其独特的脂肪酸特征 :红藻中含有大量的 2 0 :4 n- 6和 2 0 :5n- 3,其中紫球藻 R2 5含量最高 ,占总脂肪酸的4 9.8% (AA占总脂肪酸的 2 0 .5% ,EPA占总脂肪酸的 2 9.3% )。隐藻的主要脂肪酸是 16 :0、18:1n- 9、18:3n- 3、18:4 n- 3、2 0 :5n- 3、2 2 :5n- 3。与其它甲藻有别的虫黄藻 ,18:4 n- 3含量很少并且不含EPA,其主要合成的是 16 :0、18:1n- 9和 2 2 :6 n- 3。 C16和 C18的不饱和脂肪酸是本实验研究的 3株绿藻的主要脂肪酸