EFFECT OF LIGHT INTENSITY ON THE TOTAL LIPID AND FATTY ACID COMPOSITION OF SIX STRAINS OF MARINE DIATOMS

The effect of light intensity ( 1500 Ix and 5000 Ix) on the total lipid and fatty acid composition of six strains of marine diatoms Cylindrotheca fusiformis (B211 ), Phaeodactylum tricornutum (Bl14, Bl18 and B221) Nitzschia closterium (B222) and Chaetoceros gracilis (B13) was investigated.The total lipids of B13, Bl14, and B211 grown at 5000 Ix were lower than those grown at 1500 Ix. No evident changes were observed in Bl18, B221 and B222. Fatty acid composition changed considerably at different light intensity although no consistent correlation between the relative proportion of a single FA and light intensity. The major fatty acids of the 6 strains were 14:0, 16:0, 16:l(n- 7) and 20:5(n-3) . Cylindrothecafusiformis had high percentage of 20:4n - 6 (9.2 - 10.9% ) . The total polyunsaturated fatty acid /rl all 6 strains decreased with increasing light intensity. The percentage of the highly unsaturated fatty acid eicosapentaenoic acid (EPA) decreased with increasing light intensity in all strains except Chaetoceros gracilis.     

固体潮观测资料平滑处理方法的研究

固体潮观测数据在进行各种分析之前，需进行平滑处理以便消除各种突跳及错误数据。常用的平滑方法有Ｌｅｃｏｌａｚｅｔ数字滤波法和Ｎａｋａｉ方法。本文讨论了各种方法的特点及存在问题。为了经济、快速而又有效地平滑数据，提出如下建议：对于高精度的观测数据，通常使用数字滤波法处理即可；在少数情况下，数据中出现连续错数，这时可取卅天为时间间隔，在上述滤波法平滑的基础上，分段对数据进行调和分析，如果某几段调和分析的     

负载于零漂上的低频非潮汐信息的提取

重力固体潮观测资料中的零漂项，从整体上看，在缓慢地变化着，本文以朔望月的一半作为时间长工，分段对零漂进行线性模拟，之后，再进一步消除长周期潮汐波对零漂的影响，提出了零漂的变化，这是一种低频非潮汐信息，它负载于零漂之上，可能含有地震前兆。     

露天矿台阶对高密度电法勘探影响的实验研究

露天矿边坡稳定性评估是预防边坡事故发生的基本手段之一,物探结论一直作为重要依据被广泛采纳。在露天矿台阶上进行高密度电法勘探时,为研究台阶对勘探结果的影响,本文进行了水槽相似材料模拟实验,研究了待测剖面内的电流场分布。实验结果显示：待测剖面中靠近上下坡面线及清扫平台宽度线的浅部位置电流密度大,且分布均匀;中部位置电流密度变化急剧,且电流密度等高线的形状与台阶形状相似;深部位置电流密度小,变化迟缓;待测剖面上靠近坡底线的位置出现明显的电流聚集效应,台阶地形下的电流密度比在平面地形下的电流密度大,且分布不均匀。这表明应用高密度电法实测时,露天矿台阶会导致中部位置和靠近坡底线位置处的反演成果异常。     

EFFECTS OF HARVEST STAGE ON THE TOTAL LIPID AND FATTY ACID COMPOSITION OF FOUR CYLINDROTHECA STRAINS

Four strains of Cylindrotheca (B156, B164, B196, and B200) were harvested at three phases in the growth curve and their total lipid and fatty acid composition were determined. Total lipid reached highest values in the late stationary phase in B156 (24.0%), B164 (26.6%) and B200 (17.3%), in the exponential phase in B196 (15.5%). Saturated fatty acids increased with the devel-opment of the culture in B156 and B196, and reached their highest values in the late stationary phase in B156 (41.7%) and B196 (45.1%), in the early stationary phase in B164 (45.4%) and B200 (37.6%). Monounsaturated fatty acids increased with the development of the culture in B196 and B200, and reached their highest values in the late stationary phase in B196 (32.4%) and B200 (32.8%), in the early stationary phase in B164 (31.0%) and in the exponential phase in B156 (29.3%). Polyunsaturat-ed fatty acids decreased in the later phases of the culture in B164, B196 and B200, and peaked in theexponential phase in B164 (29.5%), B196 (42.9%) and B200 (37.3%), and in the early stationary phase in B156 (32.0%).     

Effect of NaNO3 Concentrations on the Growth and Fatty Acid Compositions of Nitzschia closterium and Chaetoceros gracilis

Fatty acid compositions of two strains of marine diatoms Nitzschia closterium(MACC B222)and Chaetoceros gracilis(MACCB13)have been examined.The microalgae have been grown at different initial NaNO3 concentrations(75,375,750,1125,1500,1875mg/L)and harvested at the late exponential phase.The results by one factor analysis of variance show that the NaNO3 concentrations have no significant influence on the relative growth rate of two stains of marine diatoms;the influence of NaNO3 concentrations on the fatty acids differs from species to species.The major fatty acids of two diatom strains are 14:0,16:0,16:In-7and 20:5n-3(EPA),B13 also has high percentage of 20:4n-6(6.0-9.1%).