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M.A. van Leeuwe L.A. Villerius J. Roggeveld R.J.W. Visser J. Stefels 《Marine Chemistry》2006,102(3-4):267-275
A recent development in algal pigment analysis by high-performance liquid chromatography (HPLC) is the application of automation. An optimization of a complete sampling and analysis protocol applied specifically in automation has not yet been performed. In this paper we show that automation can only be successful if the various methodological aspects of the sampling and analysis protocol are considered in coherence. We introduce an optimized protocol that involves freeze-drying of the sample, subsequent extraction in 90% acetone and the application of water-packing during analysis. The method was evaluated on both natural plankton populations and a broad spectrum of microalgal cultures: Thalassiosira weisflogii (Bacillariophyceae), Emiliania huxleyi (Prymnesiophyceae), Phaeocystis globosa and Phaeocystis antarctica (Prymnesiophyceae) and Pyramimonas sp. (Prasinophyceae). Whereas pigment extracts were unstable in methanol, with recorded chlorophyll a losses from 10% to 60% per day, pigment degradation rates in acetone were generally less than 1% over 18 h storage in the autosampler (4 °C). In addition, it was found that the extraction efficiency of acetone significantly increased upon freeze-drying prior to extraction. Increases as high as 50–60% were measured in P. antarctica. The application of water-packing of the sample during injection resulted in improved peak shape and peak separation, without diluting the pigment concentrations. Automation is especially beneficial for application in the field, when mixed algal assemblages and low biomass put a high demand on the sensitivity as well as reproducibility of the method. 相似文献
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本文简要地介绍了办公自动化的发展过程,分析了其常用的功能、特点,以及内容分类,论述了目前办公自动化系统的技术支撑和实现,并指出政府办公自动化系统在开发和应用中存在的问题和解决思路。对系统的推广使用具有一定的参考意义。 相似文献
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地下管线探测工程的资料自动化整理 总被引:1,自引:0,他引:1
地下管线探测工程一般分为野外探测和室内资料整理两阶段。内业整理包括成果表的编制、管线综合分布平面图和管线数据库。由于管线探测工程数据多,为了充分利用外业探测成果,应用Visual Basic 6.0(VB6)开发了一套地下管线管理信息系统(UTMIS),该系统实现了地下管线探测工程内业整理的自动化,极大地提高了工作效率数据精确度。 相似文献
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毫米波雷达云回波的自动分类技术研究 总被引:1,自引:0,他引:1
毫米波雷达在云探测方面比厘米波天气雷达和激光雷达具有显著优势,可获得更多的云粒子信息,是研究云特性的主要遥感探测设备。为了开展对毫米波雷达探测的云回波进行自动分类的研究,利用161次云回波的个例数据,统计得到了卷云、高层云、高积云、层云、层积云和积云6类云型的特征量和其他参量的数值范围,利用分级的多参数阈值判别方法,达到了自动分类的目标,通过与人工分类的初步验证,两种分类结果的一致性达到84%,其中,层云和积云的识别一致较低的原因在于样本数据有限,仅有6次层云和8次积云的个例样本数据。通过更多样本的处理,提取的特征参量更可靠,自动分类的准确率会得到提高,以便将基于毫米波雷达的云分类技术应用于将来的云观测自动化业务。 相似文献
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The more sensitive an analysis method the more care must be given to sample preparation. Solid phase extraction (SPE) onto RP 18 phases has established itself as a general purification and enrichment technique for trace components in aqueous samples. Various factors may thereby influence the recovery rate. For example, the ratio amount of sorbent and sample volume should be taken into account to avoid breakthrough of the investigated substance. Extracting phenylurea herbicides from drinking water, the ratio should be 1 g RP 18 sorbent per 1 L water sample. Concerning the flow rate for the fungicides procymidone, vinclozolin, and iprodione, the recovery rate decreases using higher flow rates than 3 or 6 mL/min. In the case of phenylurea herbicides, a flow rate of 10 or 14 mL/min showed best results. Even the coefficient of variation is below ± 5% at these flow rates. Furthermore, the company dependence should be considered because the recovery rate might differ by up to 40% using the nominally identical material. The same applies to the batch dependence of quality from the same company. The concentration of the eluate must also be monitored and carried out with great care to avoid decreasing the recovery rate. Considering these factors of influence, SPE is a very useful tool in sample preparation, particularly if an automated system is used. 相似文献
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Despite the growing recognition that metropolitan real estate markets are segmented spatially and that property development is still profoundly local, research has taken little notice of agents that actually initiate and propel the development process. Earlier studies that aimed at identifying metropolitan segmentation employed statistical techniques whilst ignoring the practices of agents that might shed light on the notion of segmentation. Greater Toronto’s office market, which is Canada’s largest, exemplifies a real estate market far more spatially segmented than the downtown–suburban framework, a dichotomy so extensively use in research. Locational preferences of the numerous developers that work in this market tend to be highly localized, confirming deep-rooted segmentation. Their preferences correspond to submarket classification and follow dividing lines that crisscross the metropolitan region, emphasizing the extent and the power of parochialism, which defines the nature of real estate development. 相似文献
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针对流行最广的Office Open XML 格式文档(即MS Office 2007—2013),提出一种基于冗余属性的文本数字水印算法.利用OOX(Office Open XML)文档包中部件的冗余信息来嵌入水印,并使水印与文档的格式信息绑定,可有效抵抗针对文本内容的攻击.该方法具有较强的鲁棒性和较大的嵌入容量,能够抵抗"另存为"、"清除格式"等攻击,且没有改变原始文档显示字符的任何格式信息,这是以往针对格式文档提出的水印方法做不到的,同时,也没有改变原始文档的任何语义信息,这也是以往的基于语义(自然语言)的水印方法做不到的.实验结果说明了该算法的可行性. 相似文献
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以中国华北地区五大城市办公建筑为例,利用1961—2017年气象数据和TRNSYS软件模拟的供热制冷负荷数据,评估了气候变化背景下华北地区建筑供热制冷负荷的变化。在此基础上,对模拟负荷和气象要素进行多元线性逐步回归分析,揭示了影响建筑供热、制冷负荷的主要气象因子。结果表明:1961—2017年中国华北五大城市供热负荷均呈下降趋势,降幅为0.05(石家庄)—0.13 kWh·m-2·(10 a)-1(呼和浩特);各城市制冷负荷的变化不同,仅呼和浩特为增多,增幅为0.04 kWh·m-2·(10 a)-1,其余城市制冷负荷无明显变化;从总负荷来看,各城市均呈下降趋势,降幅为0.05(太原)—0.10 kWh·m-2·(10 a)-1(呼和浩特)。由供热制冷负荷与气象要素的回归分析可知,冬季供热负荷主要受气温影响,五大城市的显著增温导致供热负荷减少;与此不同,夏季制冷负荷主要受气温、太阳辐射的共同影响,呼和浩特平均气温和太阳辐射均呈显著上升趋势,导致其制冷负荷显著增加。其他城市气温显著升高,而太阳辐射显著降低,二者的综合作用导致制冷负荷没有明显的变化趋势。总体来看,在气候变暖背景下,中国华北地区冬季供热负荷明显降低,而夏季制冷负荷并未明显增加,导致总负荷显著降低,气候变暖总体上对建筑节能有利。 相似文献