Germination ofAcacia origena,A. pilispinaandPterolobium stellatumin response to different pre-sowing seed treatments, temperature and light

Seeds ofAcacia origena,A. pilispinaandPterolobium stellatumhave a hard seed coat that hampers imbibition of water and prevents maximum, uniform and rapid germination. Therefore, the seeds were subjected to different treatments involving mechanical scarification, concentrated sulphuric acid and boiling water to break the dormancy imposed by the hard seed coat. To determine the effects of different constant temperature regimes on germination, scarified seeds were incubated on a thermogradient at 10, 15, 20, 25 and 30°C. Germination of scarified seeds was also tested in light and dark. Mechanical scarification resulted in 100% germination for theAcaciaspecies and 94% forP. stellatum. ForA. origena, sulphuric acid treatments for 60, 90 and 120 min resulted in significantly higher per cent germination (>95%) than all the other treatments. ForA. pilispina, sulphuric acid treatments for 45 and 60 min, as well as immersing seeds for 1 min in hot water, gave significantly higher per cent germination (97%) than all the other treatments. ForP. stellatum, mechanical scarification and all the acid treatments applied resulted in significantly higher per cent germination (>85%) than the control and all hot water treatments. All seeds ofP. stellatumimmersed in hot water, even for 1 min, rotted soon after sowing, indicating that they are sensitive to high temperatures. Scarified seeds of the twoAcaciaspecies germinated to a higher degree (≥90%) between 10 and 30°C, while germination of scarified seeds ofP. stellatumranged from 48 to 82% between 10 and 30°C. Scarified seeds of the three species germinated to a large extent (>70%) both in light and dark, indicating that seeds do not require light for germination.     

Ein Computerprogramm zur Orientierungsbestimmung von Kristallen aus Laue-Diagrammen

Zusammenfassung Es wurde ein Algol-60-Programm verfaßt und getestet, das bei bekannten Gitterkonstanten die Orientierung für Kristalle des rhombischen, tetragonalen, kubischen und hexagonalen Kristallsystems aus Laue-Aufnahmen bestimmt.

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A computer program to determine the orientation of crystals from laue patterns

Summary An Algol-60-program was designed, which computes the orientation of crystalls with rhombic, tetragonal, cubic or hexagonal structure from Laue photographs if the lattice constants are known. Test runs were made on the IBM 7040 computer.

     

Stable carbon isotope ratios in tree rings of co-occurring species from semi-arid tropics in Africa: Patterns and climatic signals

Although several proxies have been proposed to trace the course of environmental and climatological fluctuations, precise paleoclimate records from the tropics, notably from Africa are still sorely lacking today. Stable carbon isotopes (δ¹³C) in tree rings are an attractive record of climate. In this study, the patterns and climatic signals of δ¹³C ratios were determined on tree rings of deciduous (Acacia senegal, Acacia tortilis, Acacia seyal) and an evergreen (Balanites aegyptiaca) species, from a semi-arid Acacia Woodland in Ethiopia. δ¹³C inter-annual patterns are synchronous among the co-occurring species. A declining trend with time was observed in δ¹³C, notably for B. aegyptiaca, which could be due to anthropogenic increases in atmospheric CO₂ concentration and decrease in atmospheric δ¹³C. Tree ring δ¹³C values of all the species revealed significant negative correlation with precipitation amount but not with temperature and relative humidity. The δ¹³C series of the deciduous species shows a higher correlation (r = − 0.70 to − 0.78) with precipitation than the evergreen species (r = − 0.55). A master δ¹³C series, composed of the average of the three Acacia species, displayed stronger significant correlation (r = − 0.82) than any of the individual species δ¹³C series. The weak relationship between temperature and δ¹³C in this study indicates that photosynthetic rate is not a significant factor. Moisture stress, however, may have a direct impact on the stomatal conductance and explain the strong negative relationship between δ¹³C and precipitation. The results demonstrate the potential of δ¹³C in tree rings to reflect physiological responses to environmental changes as a vehicle for paleoclimatic reconstruction, which is important to understand tree response to past and future climate change.     

Soil seed flora, germination and regeneration pattern of woody species in an Acacia woodland of the Rift Valley in Ethiopia

The objectives of this study were to determine the composition, density and spatial distribution of the soil seed bank of woody species, as well as their regeneration pattern in two different land use systems, controlled (ranch) and open grazing, in an Acacia woodland of the Rift Valley in Ethiopia. We also compared the species composition of the soil seed bank and the above-ground vegetation to find out if differences exist in the soil seed bank and advance regeneration between the two land use systems. The germination requirements of seeds of the woody species were also investigated under laboratory conditions. Acacia senegal, Acacia seyal, Acacia tortilis, Dichrostacys cinerea and Balanites aegyptiaca were encountered in the above-ground vegetation in both systems. Seeds of only A. tortilis (90±32 seeds m⁻²) from the ranch, and A. senegal (5±3 seeds m⁻²) and A. tortilis (72±34 seeds m⁻²) from the open system were found in the soil seed bank along transects with patchy horizontal pattern. The two systems were not significantly different in density of soil seed banks of A. senegal andA. tortilis . Jaccard's similarity index showed that only a few woody species were common in the soil seed flora and above ground vegetation. However, all of the species accumulated seeds (58±43–331±130 seeds m⁻²) in/on the soil under the canopy. Very large numbers of seeds of A. tortilis (19382±9722 seeds m⁻²) and D. cinerea (1278±494 seeds m⁻²) were also found in barns. Most of the seeds recovered from the soil samples (60–80%) were found in the litter layer. Acid and mechanical scarification improved legume germination (36–99% and 60–99%, respectively) over boiling water (0–48%). Treatment means differed significantly for all the legumes (p<0·001) but not for Balanites. Height and diameter class distribution of regeneration of A. tortilis and D. cinerea in both systems and A. senegal in the open system had a negative exponential correlation (r_s=−0·5, −0·25 and −0·86, respectively). A. seyal and B. aegyptiaca showed poor regeneration. Horizontal distribution of advance regeneration of all the species was patchy. Advance regeneration of A. seyal, A. tortilis and B. aegyptiaca were not significantly different, while that of A. senegal and D. cinerea were significantly different between the two systems. Poor representation of species in the soil seed bank along transects and in the different height and diameter classes may be attributed to the low density of mature trees as well as the mode and strategy of seed dispersal. Ungulate and wind dispersed species (e.g. A. senegal, A. tortilis and D. cinerea) were highly favoured. Patchiness in the distribution of seeds and advance regeneration was also a result of endozoochory. Dispersal of non-ungulate dispersed seeds (A. senegal, A. seyal and B. aegyptiaca) was restricted to the canopy zone. Piles of seeds ofA. tortilis and D. cinerea that were found in barns were a result of consumption of their pods by cattle. High concentration of seeds in the litter layer may be due to low soil disturbance and larger size of seeds. The height and diameter class distribution of A. senegal (in the open system), A. tortilis and D. cinerea also indicated that the species have good regeneration. Results from the germination tests indicated that seeds of the legumes require pre-sowing treatments to give a rapid, uniform and improved germination. Intervention through artificial regeneration should be employed to improve the density and regeneration capacity of those species with hampered regeneration at both systems.