Screening of marine fungus from Nanji Island and activity of their metabolites against pathogenic Vibrio from Pseudosciaena crocea

Seventy-eight marine fungal strains were isolated from sediment samples collected off the coast of Nanji Island,Wenzhou,Zhejiang Province,China.Antibacterial screening using the agar disc method showed that 19 of the isolated strains could inhibit at least one pathogenic Vibrio from Pseudosciaena crocea.Subsequent screening confirmed that nine strains produced antibacterial metabolites that had activity against one or several types of pathogenic Vibrio.Strain NJ0104 had the widest antimicrobial spectrum and strong activity,particularly against Vibrio parahaemolyticus-MM0810072.A preliminary study of NJ0104 antibacterial metabolites demonstrated that they had thermal stability up to 80°C,ultraviolet stability up to 40 min and pH stability between 4.0-7.0.In addition,the antibacterial metabolites were readily soluble in butanol.To identify the specific strain,the ITS-5.8S rDNA regions of NJ0104 were PCR amplified and sequenced.Based on the combination of phenotypic and genotypic data,the strain was identified as Arthrinium sp.     

南麂岛海洋沉积物抑菌真菌的筛选及其代谢产物特性的初步研究

以7种水产病原菌为指示菌,采用菌饼法对分离自南麂岛海域的15个沉积物样品中的78株海洋真菌进行抑菌活性初筛,获得了对至少一种病原指示菌有抑菌活性的菌株19株.采用打孔扩散法对这19株真菌的发酵液进行抑菌活性复筛,共有9个菌株的次级代谢产物对一种或者几种病原指示菌有抑制作用,其中菌株NJ0104的次级代谢产物对除灿烂弧菌...