渤海沉积物中细菌群落结构和基因丰度的空间分布特征

This study investigated differences in the community structure and environmental responses of the bacterial community in sediments of the Bohai Sea.Illumina high-throughput sequencing technology and real-time PCR were used to assay the bacterial 16S rRNA genes in the surface sediments of 13 sampling stations in the Bohai Sea.The results showed that sediments at the majority of the 13 sampling stations were contaminated by heavy metal mercury.The main phyla of bacteria recorded included Proteobacteria(52.92%),Bacteroidetes(11.76%),Planctomycetes(7.39%),Acidobacteria(6.53%)and Chloroflexi(4.97%).The genus with the highest relative abundance was Desulfobulbus(4.99%),which was the dominant genus at most sampling stations,followed by Lutimonas and Halioglobus.The main factors influencing bacterial community structure were total organic carbon,followed by depth and total phosphorus.The content of lead,cadmium,chromium,copper and zinc had a consistent effect on community structure.Arsenic showed a negative correlation with bacterial community structure in most samples,while the impact of mercury on community structure was not significant.The bacterial community in sediment samples from the Bohai Sea was rich in diversity and displayed an increase in diversity from high to low latitudes.The data indicated that the Bohai Sea had abundant microbial resources and was rich in bacteria with the potential to metabolize many types of pollutants.     

印度尼西亚峇淡岛海域鱾新本尼登虫（Neobenedenia girellae）形态学及28S rRNA分子鉴定

本研究通过结合形态学与DNA分子标记等生物学手段，对印度尼西亚峇淡岛海域海水鱼类养殖中的本尼登虫(Isolate Batam, IB)进行形态学分析和种类鉴定。IB虫株在形态上与鱾新本尼登虫(Neobenedenia girellae)相似，属于新本尼登属。28S rRNA序列扩增得到序列为394 bp，与其他本尼登虫属比对相似性在85.86%～99.47%之间。进化树构建结果显示IB虫株与其他珻氏新本尼登虫(Neobenedenia melleni)和鱾新本尼登虫构成一簇，而本尼登虫自成一簇。综上所述，本研究结果支持鱾新本尼登虫与珻氏新本尼登虫为同种异名的分类学观点，并将IB虫株定种为鱾新本尼登虫。