Effects of varying densities on serum reproductive parameters in pen-reared juvenile female rainbow trout <Emphasis Type="Italic">Oncorhynchus mykiss</Emphasis> farms

The primary goal of this study was to assess the effect of varying densities on serum reproductive parameters of immature rainbow trout Oncorhynchus mykiss. Experimental trout were maintained in intensive, pen-reared farms for 300 days in fresh water reservoirs. Initial densities were 4.6, 6.6, and 8.6 kg/m³ (40, 60, 80 ind./m³), indicated as SD1, SD2, SD3, and final densities were 31.1, 40.6, 49.3 kg/m³, respectively. A summary of the ovarian stages were observed by histological examination. Serum E₂ (estradiol), T (testosterone) were evaluated by radioimmunoassay and FSH (follicle-stimulating-hormone), LH (luteinizing-hormone), vitellogenin, 17α,20β-P (17α,20βdihydroxy4-pregnen-3-one) were measured by enzyme-linked immunosorbent assay. Our findings demonstrated that ovarian development were retarded (from stage III to stage IV) at highest rearing density (SD3) after 180 days of intensive culture (over 40.6 kg/m³). In addition, we observed an inverse relationship between serum reproductive parameters and rearing density. Furthermore, compared to serum reproductive parameters of SD1, E₂, T, FSH, vitellogenin, 17α,20β-P, GSI and LH of two higher density groups decreased firstly and significantly at 60 (over 15.9 kg/m 3 ), 180 (over 31.7 kg/m 3 ), 180 (over 40.6 kg/m³), 240 (over 36 kg/m³), 240 (over 36 kg/m³), 240 (over 45 kg/m³) and 300 (over 49.3 kg/m³) days, respectively. Comparing serum reproductive parameters within the same ovarian development stage of rainbow trout from varying densities revealed that higher population density also led to significantly lower overall serum reproductive parameters. Overall, this study presents the reproductive, endocrinological parameters of juvenile female rainbow trout at high rearing densities and indicates the need for rainbow trout (114.44±5.21 g, 19.69±0.31 cm) that are initially stocked at 6.6 or 8.6 kg/m³ should be classified and subdivided into lower density after 180 days of farming (not over 31.7 kg/m³).     

急性温度胁迫对虹鳟血细胞和血清生化组分的影响

本文研究急性温度胁迫对虹鳟血液生理和生化指标的影响,将虹鳟放入高温24℃和低温6℃水中热应激2h,然后放入16℃水中饲养,检测血液三类细胞数量及血清生化组分。结果表明:温度胁迫后,血液红细胞(wBC)和血红蛋白都呈现先降低后升高的趋势,低温组在12h降至最低,高温组在6h降至最低;白细胞在0h有不显著升高,其余时间与对照组没有显著差异;温度胁迫后,2个处理组血糖(GLU)在整个实验过程中都显著低于对照组;总蛋白(TP)和白蛋白(ALP)在温度胁迫后都呈现先降低后升高的趋势,低温组TP在6h达到最低值,ALP在12h达到最低值,高温组TP和ALP都在6h达到最低值;温度胁迫后,两处理组胆固醇(TC)都呈现先降低后升高再降低的趋势,在24h达到最大值;两处理组甘油三酯(TG)胁迫后都低于对照组,但没有明显变化趋势;温度胁迫后,无机物钙离子(Ca~(2+))只有高温组在24h显著降低,磷(P)只在胁迫后0h显著升高,其余时间两者没有明显变化。急性温度胁迫对虹鳟血细胞和血清生化组分产生了不同程度影响,为虹鳟运输、养殖条件优化等研究提供科学依据。