北极薄冰-煎饼冰区域中不同重力波驱动形成海流的敏感性分析

A coupled ocean-ice-wave model is used to study ice-edge jet and eddy genesis during surface gravity wave dissipation in a frazil-pancake ice zone. With observational data from the Beaufort Sea, possible wave dissipation processes are evaluated using sensitivity experiments. As wave energy dissipated, energy was transferred into ice floe through radiation stress. Later, energy was in turn transferred into current through ocean-ice interfacial stress. Since most of the wave energy is dissipated at the ice edge, ice-edge jets, which contained strong horizontal shear, appeared both in the ice zone and the ocean. Meanwhile, the wave propagation direction determines the velocity partition in the along-ice-edge and cross-ice-edge directions, which in turn determines the strength of the along-ice-edge jet and cross-ice-edge velocity. The momentum applied in the along-ice-edge(cross-ice-edge)direction increased(decreased) with larger incident angle, which is favorable condition for producing stronger mesoscale eddies, vice versa. The dissipation rate increases(decreases) with larger(smaller) wavenumber, which enhances(reduces) the jet strength and the strength of the mesoscale eddy. The strong along-ice-edge jet may extend to a deep layer(> 200 m). If the water depth is too shallow(e.g., 80 m), the jet may be largely dampened by bottom drag, and no visible mesoscale eddies are found. The results suggest that the bathymetry and incident wavenumber(magnitude and propagation direction) are important for wave-driven current and mesoscale eddy genesis.     

牡蛎酶解工艺优化及其酶解产物对小鼠睾酮分泌的影响

【目的】优化太平洋牡蛎(Crassostreagigas)酶解工艺条件,并研究酶解产物及其超滤组分对体外培养的睾丸间质细胞增殖及睾酮分泌的影响。【方法】以多肽得率为参考标准,对木瓜蛋白酶酶解牡蛎的加酶量、初始pH、酶解时间、酶解温度进行单因素及正交实验优化;通过MTT法及酶联免疫吸附试验(ELISA)检测不同浓度牡蛎酶解产物及其超滤组分对TM3小鼠睾丸间质细胞增殖及睾酮分泌的影响。【结果】木瓜蛋白酶最佳酶解条件为加酶量3 000 U/g,初始pH 6. 5,酶解温度62.5℃,酶解时间4.5 h,在此条件下其多肽质量浓度为14.95 mg/mL;牡蛎酶解液及其超滤组分在0.25～1.00 mg/mL质量浓度范围内,与空白对照组相比,能够显著增加TM3细胞的增殖活性(P0.05),且具有浓度依赖性和时间依赖性。其中,5 ku和5～10 ku超滤组分细胞增殖活性及促进睾酮分泌活性均最强。【结论】牡蛎酶解产物及其超滤组分可有效增强小鼠睾丸间质细胞增殖活性并促进其分泌睾酮。