Erratum to: Purification and characterization of iron-cofactored superoxide dismutase from Enteromorpha linza

<正>Erratum to:Chinese Journal of Oceanology and Limnology Vol.31 No.6,P.1190-1195,2013http://dx.doi.org/10.1007/s00343-013-3049-3The supporting grant information on the footnote of the original version of this article needs to be altered and updated.The new information is given below.     

浒苔孢子放散与附着萌发特性及其干出适应性的初步研究

研究了浒苔（Enteromorpha prolifera）孢子的放散与附着时限及其对附着基质的选择性,并探讨不同温度的干出处理对浒苔活力、孢子释放水平及其萌发的影响。实验结果表明：浒苔在20℃（黑暗）干出12h后连续放散孢子时限约为1～10h,放散高峰出现在4h左右;浒苔孢子附着时限约为1～12h,附着高峰出现在6h左...     

不同氮磷水平对缘管浒苔生长及光合作用的影响

近岸海域的富营养化是被广泛关注的重要海洋生态环境问题,其中N和P是富营养化过程主要的2种营养盐.本实验旨在探讨N和P对缘管浒苔的生长、光合作用以及可溶性蛋白含量的影响.结果表明:添加N和P都显著增加藻体的光合作用速率、可溶性蛋白含量,最终显著提高藻体的相对生长率.缘管浒苔对N和P的利用可以在一定范围内相互弥补.这表明缘...     

低盐在短时间内对绿潮浒苔(Ulva prolifera)氮磷吸收的动态影响

我国黄海出现的绿潮发源于黄海南部苏北辐射沙洲紫菜养殖区,苏北沙洲区濒临的沿岸河网众多,来自沿岸径流的淡水携带大量氮磷等营养盐间歇性入海,导致海水的富营养化并伴随着盐度的周期性波动。本研究通过模拟实验研究低盐度(15和5)对浒苔吸收氮盐(NO_3~–-N和NH_4~+-N)和磷盐(PO_4~(3–)-P)的影响,主要发现:与盐度30相比,在低盐度(15和5)时,浒苔对NO_3~–-N的1h最大吸收速率(V_(max))和亲和力(V_(max)/Ks)分别提高280%和350%左右,半饱和常数(Ks)下降15%左右,并能够维持对NO_3~–-N的高效吸收(24h);盐度15和5时,浒苔对NH_4~+-N的1h最大吸收速率(V_(max))分别提高40%和200%,亲和力(V_(max)/Ks)分别提高20%和180%, Ks分别提高15%和30%,但是盐度降低对NH_4~+-N的长效吸收产生负面影响,甚至在盐度5条件下出现吸收高浓度NH_4~+-N后再释放的现象;与盐度30相似,盐度15条件下浒苔能够快速吸收PO_4~(3–)-P,而盐度5则导致藻细胞内的PO_4~(3–)-P在早期阶段快速流失,并在后期不能有效吸收PO_4~(3–)-P。本实验的结果表明,降低盐度有利于浒苔对氮源的快速吸收,在盐度15下浒苔能够实现对硝酸盐和磷盐的高效吸收。     

Inhibitory activity of an extract from a marine bacterium Halomonas sp. HSB07 against the red-tide microalga Gymnodinium sp. （Pyrrophyta）

In recent years, red tides occurred frequently in coastal areas worldwide. Various methods based on the use of clay, copper sulfate, and bacteria have been successful in controlling red tides to some extent. As a new defensive agent, marine microorganisms are important sources of compounds with potent inhibitory bioactivities against red-tide microalgae, such as Gymnodinium sp. （Pyrrophyta）. In this study, we isolated a marine bacterium, HSB07, from seawater collected from Hongsha Bay, Sanya, South China Sea. Based on its 16S rRNA gene sequence and biochemical characteristics, the isolated strain HSB07 was identified as a member of the genus Halomonas. A crude ethyl acetate extract of strain HSB07 showed moderate inhibition activity against Gymnodinium sp. in a bioactive prescreening experiment. The extract was further separated into fractions A, B, and C by silica gel column chromatography. Fractions B and C showed strong inhibition activities against Gymnodinium. This is the first report of inhibitory activity of secondary metabolites of a Halomonas bacterium against a red-tide-causing microalga.     

脊尾白虾精子体外保存的研究

采用精子存活率和顶体酶活力两种评价精子质量方法,研究了不同稀释剂与抗冻保护剂对脊尾白虾(Exopalaemon carinicauda)精子体外保存效果的影响。结果表明：稀释剂Ⅰ-壬氏液为脊尾白虾精子体外保存的最佳稀释剂,4℃下保存4 d后,稀释剂Ⅰ组精子存活率为71.50%,顶体酶活力为1.803μIU/106,两种指标值均高于其他实验组,各组精子存活率与顶体酶活力之间相关性显著γ=0.846(P<0.05);在液氮-196℃下保存2 d后,稀释剂Ⅰ组精子存活率达83.50%,顶体酶活力为2.507μIU/106,两种指标值均高于其他各组以及4℃下稀释剂Ⅰ组同期保存效果,各组精子存活率与顶体酶活力之间相关性极显著,γ=0.862(P<0.01)。15%DMSO为脊尾白虾精子超低温保存的最佳抗冻保护剂,此条件下超低温保存2 d后,精子存活率为81.32%,顶体酶活力为1.385μIU/106,各组精子存活率与顶体酶活力之间相关性极显著,相关系数γ=0.864(P<0.01)。     

坛紫菜叶状体营养细胞与生殖细胞叶绿素荧光特性比较

利用叶绿素荧光技术对坛紫菜(Porphyra haitanensis)叶状体营养细胞和生殖细胞的光能利用特性进行了比较。结果表明:坛紫菜叶状体营养细胞和雌性生殖细胞的实际量子效率(ΔF/Fm′)差异不明显,但显著高于雄性生殖细胞;快速光曲线测定表明雌、雄藻体营养细胞的最大相对电子传递速率(rETRmax)相近,但显著高于雄性生殖细胞;不同生殖细胞半饱和光强(Ik)无显著差异;在生长光强下,营养细胞和雌性生殖细胞rETR、光化学猝灭(qP)和非光化学猝灭(NPQ)差异不明显,而雄性生殖细胞rETR、qP等荧光参数均显著低于营养细胞和雌性生殖细胞。本文表明坛紫菜叶状体营养细胞和雌性生殖细胞具有较高的光能利用能力,能够将吸收的光能多数用于电子传递,而雄性生殖细胞对光能的利用能力较低。     

盐生能源植物菊芋研究进展

种植抗盐耐海水经济作物是合理开发利用沿海滩涂、海水资源、耐盐植物的有效措施之一.就盐生能源植物菊芋的生物学特性、用途及其耐盐耐海水性研究进展,以及不同收获时间和储藏温度对菊芋块茎干物质、总可溶物及糖和菊粉组分的影响、微生物菊粉酶对菊粉水解研究进展进行了论述和展望.     

Purification and characterization of iron-cofactored superoxide dismutase from Enteromorpha linza

A superoxide dismutase was purified from Enteromorpha linza using a simple and safe procedure, which comprised phosphate buffer extraction, ammonium sulphate precipitation, ion exchange chromatography on Q-sepharose column, and gel filtration chromatography on Superdex 200 10/300GL. The E. linza superoxide dismutase （E/SOD） was purified 103.6-fold, and a yield of 19.1% and a specific activity of 1 750 U/rag protein were obtained. The SDS-PAGE exhibited E/SOD a single band near 23 kDa and the gel filtration study showed E/SOD＇s molecular weight is near 46 kDa in nondenatured condition, indicating it＇s a homodimeric protein. E/SOD is an iron-cofactored superoxide dismutase （Fe-SOD） because it was inhibited by hydrogen peroxide, insensitive to potassium cyanide. The optimal temperature for its maximal enzyme activity was 35℃, and it still had 29.8% relative activity at 0℃, then E/SOD can be classified as a cold-adapted enzyme. E/SOD was stable when temperature was below 40℃ or the pH was within the range of 5 10. The first 11 N-terminal amino acids orE/SOD were ALELKAPPYEL, comparison of its N-terminal sequence with other Fe-SOD N-terminal sequences at the same position suggests it is possibly a chloroplastic Fe-SOD.