The DNA de-methylating agent 5-azacytidine does not restore CYP1A induction in PCB resistant Newark Bay killifish (Fundulus heteroclitus)

Newark Bay (NB) killifish (Fundulus heteroclitus) have been chronically exposed to environmental contaminants that activate the aryl hydrocarbon receptor (AHR) and are tolerant to toxic effects and CYP1A induction provoked by AHR ligands. Resistance to CYP1A induction could be due to an epigenetic mechanism such as DNA methylation. We measured in-ovo CYP1A catalytic activity (ethoxyresorufin-O-deethylase, EROD) in NB and reference site killifish embryos aqueously exposed to various concentrations of the de-methylating agent 5-azacytidine, 5-AC (5, 50 and 500 μ(micro)M) with or without 0.2 μ(micro)g/l of the CYP1A inducer 3,3^′,4,4^′,5 pentachlorobiphenyl (IUPAC PCB126). Neither PCB126 alone, nor PCB126 plus 5-AC, induced EROD above levels in vehicle treated Newark Bay fish. In reference site fish, the same PCB126 dose provoked a 7.4-fold EROD induction relative to controls. We conclude that Newark Bay killifish are resistant to CYP1A induction by co-planar PCBs during early embryological development and our data suggests that DNA methylation does not play a critical role in resistance to CYP1A induction in this model.     

8000 years of vegetation history in the northern Iberian Peninsula inferred from the palaeoenvironmental study of the Zalama ombrotrophic bog (Basque‐Cantabrian Mountains,Spain)

This paper focuses on pollen, spores, non‐pollen palynomorphs (NPPs) and certain geochemical elements from the ombrotrophic blanket bog of Zalama (Basque‐Cantabrian Mountains, northern Iberian Peninsula), with the support of a robust chronology based on 17 AMS ¹⁴C dates. The main results related to the last 8000 years show that, during the early middle Holocene, pines and deciduous forests were the most extensive tree formations. At the beginning of the succession, pines reach 44%, showing regional presence, whereas after 7600 cal. a BP, deciduous forests were particularly abundant. From c. 6500 cal. a BP the pollen diagram constructed from our samples shows the first anthropogenic evidence, linked with the new economic practices related to the Neolithic of the Basque‐Cantabrian Mountains. From 3300 cal. a BP the expansion of Fagus sylvatica is particularly clear, and has since then become one of the dominant forest species in this region. We also discuss the Holocene evolution of other noteworthy plant communities in southwestern Europe, such as Taxus baccata, Juglans and shrublands.     

A primitive euhadrosaurian dinosaur from the uppermost Cretaceous of the Ager syncline (southern Pyrenees,Catalonia)

New hadrosaurid material is recorded from Fontllonga (Ager syncline, province of Lleida), in the Catalonian Pyrenees, comprising a dentary with part of the dental battery, recovered from Late Maastrichtian strata (Tremp Formation), close to the presumed Cretaceous/Tertiary boundary. This hadrosaurid is more derived than is Telmatosaurus transsylvanicus from the Haeg Basin (Romania), but lacks a number of features that diagnose the clade Hadrosaurinae plus Lambeosaurinae. It is attributed to the Euhadrosauria on the basis of the following synapomorphies: dentary teeth not recurved distally, narrow dentary crowns, and denticles not supported by subsidiary ridges. The phylogenetic relationships of the best-known iguanodontids and hadrosauroids are evaluated using mandibular and dentary tooth characters. On the basis of a cladistic analysis, the family of Hadrosauridae is diagnosed by more than 29 dentary tooth positions, parallel-sided vertical furrows formed by dentary alveoli, and absence of caudal secondary ridges. Within Hadrosauridae, successive sister-groups of Hadrosaurinae plus Lambeosaurinae are the Fontllonga taxon and Telmatosaurus. The clade Hadrosaurinae plus Lambeosaurinae is characterised mainly by a coronoid process inclined rostrally and by the absence of secondary ridges on dentary crowns. The Fontllonga find suggests the diversity of European hadrosaurids at the end of the Cretaceous to have been greater than previously thought. Moreover, the primitive character of European hadrosaurids as compared to western North American and Asian relatives may be explained in terms of geographical isolation during the Late Cretaceous.     

Effects of protandric sex change on radula,pedal morphology,and mobility in Crepidula fecunda (Gastropoda: Calyptraeidae)

Protandrism was confirmed in Crepidula fecunda Gallardo 1979 by histological analysis of the gonad. Individuals were classified as immature (12 mm or less in shell length), male (13–26 mm), intersex (25–28 mm), and female (over 26 mm). There was an evident loss of mobility with growth— large females became completely sessile. The presence of epithelial glands in mobile individuals was associated with the need for lubrication during crawling, whereas the proliferation of subepithelial glands in sessile females was associated with adhesion. Mobility of immature and male individuals was related to grazing activities on biofilms, and in males was also associated with copulation. Grazing activity of motile individuals was carried out so that the radula teeth did not come into direct contact with the substratum, but rather harvested the biofilm surface. Sessile females used the radula to rasp the area under their shells, thus cleaning the substratum in preparation for oviposition. Since this process required that the radula enter into direct contact with the substratum, it suffered abrasion of radular teeth and loss of radular denticles, producing important alterations in its appearance in mature females. A complex interaction was observed between size, mobility, histological changes in the foot, activity, and tooth‐wear of the radula, which have been related to the sessile habit, sex change, and the oviposition process in this species.     

Uvaichnites riojana: A new crane-like bird ichnotaxon from the lower Miocene of La Rioja (Ebro Basin,Spain)

A new bird ichnotaxon found in Cenicero (La Rioja, Ebro Basin, Spain) is described here. The footprints are preserved in sandstone beds in a central-distal alluvial fringe with a mud-dominated floodplain, located in the transition unit between the Nájera and Haro formations. This level is positioned between the Y and Z local Agenian biozones (lower Miocene). The footprints were preliminarily studied in another work and considered as an indeterminate ichnotaxon. Uvaichnites riojana ichnogen. nov. and ichnosp. nov. is a tridactyl footprint characterized by a prominent central pad, very large to enormous sized, with unjointed toes at the proximal end. These features differ from Aquatilavipes, Aviadactyla, Avipeda, Ludicharadripodiscus, Fuscinapeda, and Ornithotarnocia of the Avipedidae morphofamily. U. riojana is considered to be similar to common crane (Grus grus) footprints in the Gruidae family. There are a few references about this family in the Iberian Peninsula and Balearic Islands and this find could confirm the presence of Gruidae since at least the lower Miocene.     

Coastal ridge constructive processes at a multi-decadal scale in Barreta Island (southern Portugal)

Multiple ridges across prograding coasts may display variable geometries, commonly expressed through varying elevations. Changes in ridge elevation have been traditionally related to the occurrence of fluctuating progradation rates, which might, in turn, be driven by shifting environmental conditions. Here, we explore the geometry and growth mechanisms of multiple ridges, generated at Barreta Island (Ria Formosa, southern Portugal), as a consequence of the rapid progradation of the island over the last 70 years, following the artificial fixation of the downdrift Faro-Olhão inlet with jetties in 1955. The variability in the morphology of these features was analysed in combination with available wind and wave data, in order to better distinguish growth mechanisms and understand the main parameters determining the final geometry of the observed ridges. The results suggest that (1) most of the identified ridges fall in the beach ridge classification, as they have been mostly built by marine processes, and (2) the parameters derived from, or closely related to wave climate variability (e.g. progradation rates, storm occurrence) can jointly explain most of the observed morphological changes, while aeolian processes played a secondary role. Indeed, ridge geometry appears mainly controlled by progradation rates, with higher ridges associated with lower progradation rates. Progradation rate, in turn, is mostly related to longshore wave power, storminess, and the occurrence storm groups. Yet, the final configuration of ridges can also be affected by runup levels and onshore winds. Therefore, establishing the relation between ridge geometry and wave climate is not a straightforward task, because of the complex processes and interactions that control coastal morphodynamics. © 2019 John Wiley & Sons, Ltd.     

Selecting parameters for the environmental interpretation of peat molecular chemistry – A pyrolysis-GC/MS study

A number of samples from a deep peat bog in Tierra del Fuego were analyzed using pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS) in order to extract parameters that might be used to interpret the peat chemistry in terms of vegetation change, anaerobic and aerobic decomposition, and fire incidence. The choice of parameters was based on factor analysis of 177 pyrolysis products, quantified for 13 samples, separated into extract and residue, as well as the total samples. Factor analysis of extracts, residues and total samples yielded similar classifications in terms of vegetation and decomposition. Pyrolysis products and ratios that most clearly differentiated samples were used to interpret the depth profile. Although interpretation was not always straightforward, indications of parameters to describe vegetation shifts, aerobic and anaerobic decomposition, and fire largely coincided. These parameters will be used in a forthcoming study for a more complete interpretation of the peat profile.