Effects of probiotic on microfloral structure of live feed used in larval breeding of turbot Scophthalmus maximus

The effects of an exogenous probiotic( Bacillus amyloliquefaciens) on microbial community structure of B ranchionus plicatils and A rtemia sinica were evaluated in this study during turbot( Scophthalmus maximus) larval breeding. The analysis and comparison of the microfloral composition of live feed with probiotic was conducted using the Illumina HiSeq PE250. The abundance of microbial species and diversity of microflora in live feed with B. amyloliquefaciens were higher than those in the control. The microfloral composition was similar among the three replicate experimental groups of B. plicatils compared with the control after enrichment. L actococcus, Pseudoalteromonas, and Alteromonas were always dominant. Additionally, some other bacterial species became dominant during the enrichment process. The microbial community during nutrient enrichment of A. sinica was rather similar among the three control replicates. Relative abundance of Cobetia sp., the most dominant species, was 54%–65.2%. Similarity in the microbial community was still high after adding B. amyloliquefaciens. Furthermore, P seudoalteromonas and Alteromonas replaced Cobetia as the dominant species, and the abundance of Cobetia decreased to 4.3%–25.3%. Mean common ratios at the operational taxonomic unit level were 50%–60% between the two B. plicatils and A. sinica treatments. Therefore, the microbial community structure changed after adding B. amyloliquefaciens during nutrient enrichment of B. plicatils or A. sinica and tended to stabilize. Additionally, the abundance of V ibrio in any kind of live feed was not significantly dif ferent from that in the control. These results will help improve the microflora of B. plicatils and A. sinica and can be used to understand the multiple-level transfer role of probiotic species among probiotic products, microflora of live feed, and fish larvae.     

DEVELOPMENT STRATEGIES OF WATER AND LAND RESOURCES IN THE HEXI REGION,CHINA

ＤＥＶＥＬＯＰＭＥＮＴＳＴＲＡＴＥＧＩＥＳＯＦＷＡＴＥＲＡＮＤＬＡＮＤＲＥＳＯＵＲＣＥＳＩＮＴＨＥＨＥＸＩＲＥＧＩＯＮ，ＣＨＩＮＡ肖洪浪，高前兆，李福兴ＤＥＶＥＬＯＰＭＥＮＴＳＴＲＡＴＥＧＩＥＳＯＦＷＡＴＥＲＡＮＤＬＡＮＤＲＥＳＯＵＲＣＥＳＩＮＴＨＥ...     

基于可靠度理论的黄土高边坡优化设计

对黄土高边坡进行可靠性分析，应用数学原理和优化原理，建立了黄土高边坡的优化模型，对铜黄一级公路黄土高边坡进行分析验证。结果表明，该方法计算的结果与实际较接近．应用中易操作。     

博斯腾湖水矿化度趋势面分析初探

本文采用多项式作为趋势面分析数学模型,对博斯腾湖1984年湖水矿化度初步分析表明,博斯腾湖水矿化度分布主要受入湖盐分,入湖水流及湖水循环的影响。同时趋势面显示了入湖盐分及水流的运动路径和扩散情况,从而给出了它们的水文动态特征,取得了比较理想的拟合结果,这是以往传统方法难以实现的。     

气候变化对塔里木河来自天山的地表径流影响

塔里木河水资源主要来自天山南坡两条源流,选择西段阿克苏河和中段开都河-孔雀河作为研究区.1956-2003年研究河源山区气温呈持续升温且降水波动增加的趋势,其中1995-2003年升温强劲,升温速率高出48 a期间平均的3倍以上;降水自1986年后持续增加,20世纪90年代较80年代增幅达18%,并显示出河源山区湿岛向塔里木盆地扩展.因高山缺少气象观测,出山径流过程变化可以综合反映中高山带的气候变化.塔里木河来自天山的地表径流在1986-2003年间持续增长,以冰川融水补给为主的库玛拉克河,1994年以来年径流量增加已在前期平均值基础上提升了一个台阶;开都河以降水径流补给为主,1986-2002年出现了观测记录以来的丰水期,并使1986年后博斯腾湖水位快速上升,恢复到1958年记录的最高水位以上.两河年径流变化趋势基本相似,但也显示有西、中段的气候变化局部差异,出现丰枯水期的不一致;然而,在近16 a升温过程中,年径流增长幅度和快慢相近.     

江苏潮滩沉积物激光粒度仪与移液管-筛析分析结果的对比

激光粒度仪的广泛应用带来了与历史数据的对比问题,因此需要建立激光粒度仪与早期分析结果之间的关系。根据江苏海岸潮滩沉积物样品的激光粒度仪和移液管-筛析法分析,对粒度参数、粒度组分等进行了对比,对两种方法的差异进行了分析。结果表明,对于江苏潮滩沉积物粒度参数中平均粒径的激光粒度仪与移液管-筛析分析结果之间有良好的线性关系;筛分法测得的粗颗粒物质较激光法偏少,而移液管法法测得的细颗粒物质较激光法偏多;将样品分类之后再进行两种方法所获粒度参数的回归分析,相关性得以提高,说明不同粒度组成的沉积物对分析结果的对比有不同的影响。两种方法之间的换算关系不仅与研究区域有关,而且与沉积物本身的粒度组成有关。     

冈底斯新近纪钾质火山作用:消减沉积物折返的地球化学与Sr-Nd-Pb同位素证据

在冈底斯岩浆岩带的羊八井地区,林子宗火山岩系上部出现小规模的粗面质火山岩.岩石学与地球化学研究表明粗面岩与时空密切相关的大体积安山质-英安质-流纹质火山岩属于不同的火山岩系:碱性钾质系列与钙碱性系列.详细的地球化学研究证明林子宗晚期小体积钾质岩具有独立的岩浆源区,而非早期钙碱性系统低压岩浆过程演化的产物.粗面质熔岩SiO2含量为62.91%～64.63%,具有高K2O(7.52%～8.05%)、Al2O3(16.64%-17.35%)、低TiO2(0.59%～0.68%)与MsO(0.15%～0.77%).钾质熔岩富集LILE与LREE,亏损HFSE,具有高Rb/Sr(1.1～2.3)、Th/La(0.59～0.80)、Th/U(6.3～10.9)和低Ce/Pb(4.1～6.2)与Ba/Th(4.3～14.5)比值,其同位素组成变化范围分别是87Sr/86Sr(I)=0.7068～0.7075、143Nd/144Nd(I)=0.51241～0.51252、206Pb/204Pb=18.87～18.95、207Pb/204Pb=15.63～15.70和208Pb/204Pb=39.24～39.68.粗面岩的地球化学与同位素特征表明冈底斯新近纪钾质火山岩来源于角闪岩相的消减沉积物熔融.因此,羊八井新近纪钾质火山熔岩提供了印度-亚洲大陆碰撞早期消减沉积物折返的证据.