Observation of clouds and solar radiation over the Pacific Ocean as relation to global climate

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Atmospheric input of trace elements to the western Pacific Ocean and the Kuroshio ocean area

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Study on the characteristics of the marine boundary layer in the Equatorial Pacific

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中国卤虫早期胚胎发育相关基因的研究--mRNA差异显示技术的三种不同染色方法比较

利用mRNA差异显示技术研究中国孤雌生殖卤虫胚胎发育的相关基因，并对中国孤雌生殖卤虫差异显示中SYBR Green Ⅰ、硝酸银和溴化乙锭三种染色方法进行了比较。以三种单碱基锚定引物对发育0h，4h，8h，12h，16h和10天的中国孤雌生殖卤虫的RNA进行逆转录和26种随机引物进行PCR反应，经非变性的6％聚丙稀酰胺凝胶电泳后，分别用SYBR Green Ⅰ、硝酸银和溴化乙锭染色，最后进行差异条带的回收和再扩增。结果表明：(1)6种样本的DD-PCR产物显示长200-3．0k不等的扩增片段，条带清晰，并呈现出有无和强弱的变化(2)SYBR Green Ⅰ和硝酸银染色的灵敏度比溴化乙锭染色的灵敏度高(3)经SYBR Green Ⅰ和溴化乙锭染色的凝胶的回收和再扩增效率比硝酸银染色的高(4)水煮法和NAP-25过柱法对凝胶片段的回收效果几乎相当。利用mRNA差异显示技术研究中国孤雌生殖卤虫胚胎发育相关基因时，得到了较好的扩增片段；三种染色方法中SYBR Green Ⅰ染色灵敏度较高，并且SYBR Green Ⅰ染色有利于凝胶片段的回收和再扩增。     

Characterization of water bursting and discharge into underground mines with multilayered groundwater flow systems in the North China coal basin

A conceptual framework for characterization of water bursting and discharge into underground mines with multilayered groundwater flow systems is presented, based on the features of the site conditions and analyses of the water bursting and mine inundation events of the North China coal basin. Comprehensive analyses of the hydrogeological conditions for establishment of the three-dimensional groundwater flow systems of the North China coal basin revealed different vertical hydraulic connection paths or channels. These connections include karst collapse columns, fault or fracture zones, buried weathering zones, and fracture networks in aquitards, within the multilayered groundwater flow systems. Also examined, was the effect of the primary features of the flow system and those different connection paths on water bursting and discharge into the underground coal mines. It was demonstrated that appropriately identifying and adequately understanding the three-dimensional multilayered groundwater flow systems and those various vertical hydraulic connection mechanisms are critical for appropriately preventing water bursting hazards and predicting water discharge into underground mines. A valuable guideline is presented on characterization of water bursting and discharge into underground mines with multilayered groundwater flow systems.

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Resumen Un cadre de travail conceptuel pour la caractérisation du jaillissement et de la vidange des eaux dans les mines à plusieurs niveaux d'écoulement est présenté, basé sur les conditions et l'analyse sur site du jaillissement des eaux et des inondations dans les bassins charbonneux du Nord de la Chine. L'analyse compréhensive des conditions hydrogéologiques des systèmes d'écoulement tridimensionnel de ces bassins, révèle des trajets ou des chenaux qui sont autant de connections hydrauliques verticales. Ces connections comprennent des effondrement karstiques en colonne, des zones de fractures et de failles, des zones d'altération, des réseaux de fractures dans les aquitards, dans le système hydrogéologique multicouche. L'influence des caractéristiques primaires du système d'écoulement et de ces différentes connections sur le jaillissement et la vidange dans les mines, est également examiné. Il a été démontré que l'identification appropriée et la compréhension adéquate du système d'écoulement souterrain multicouche et tridimensionnel, et des mécanismes variés de connection hydraulique verticale est critiquée pour prévenir de manière appropriée les risques de jaillissement et de vidange dans les mines. Des guidelines valorisables sont présentées, pour la caractérisation du jaillissement et de la vidange dans de tels systèmes.

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Résumé Se presenta un marco conceptual para la de caracterización de la despresurización y descarga de agua a minas subterráneas con sistemas de acuíferos de múltiples niveles, el cual se basa en las condiciones del sitio, análisis de la despresurización de agua e inundaciones de minas en la cuenca de carbón del norte de la China.Un análisis comprehensivo de las condiciones hidrogeológicas para el establecimiento de los sistemas de flujo de agua de tri-dimensionales en la cuenca de carbón del norte de la China ha revelado diferentes canales hidráulicos verticales. Estas conexiones incluyen columnas de karst colapsadas, fallas o zonas de fracturas, zonas de regalita hundida y redes de fracturas en zonas de poca permeabilidad dentro de los sistemas de flujo de múltiples niveles.También se investigó el efecto de los rasgos primarios del sistema de flujo y los diferentes canales conectivos sobre la despresurización y descarga de agua a minas subterráneas. Se demostró que el entendimiento e identificación adecuada de los sistemas de acuíferos de múltiples niveles en tres dimensiones es crítico para prevenir apropiadamente la despresurización y descarga de agua a minas subterráneas. Se presentan lineamientos valiosos en relación con la despresurización y descarga de agua a minas subterráneas en sistemas de flujo de múltiple nivel.

     

青藏高原近地面层微气象学特征

利用1998年5月-7月在改则、当雄和昌都三测站获得的近地面层气象要素变化的观测资料，分析了青藏高原近地面层风速、温度和湿度日变化特征及廓线规律，发现高原近地面层微气象学特征具有自己的特点；同时还讨论了高原近地面层白天出现的逆湿现象。     

一次冬季锋面暴风雪天气过程的斜压边界层特征的观测分析

对STORM-FESTIOP17一次冬季锋面暴风雪天气过程的斜压边界层结构演变及特征进行了分析。发现：暖湿空气沿锋面抬升凝结成云，产生降水过程中释放的大量潜热显著增加锋两侧的水平温度差异，产生锋生。与锋生相伴，在锋前产生低空急流和高空急流。当锋生至最强时，锋两侧温差可达20K，锋前低空急流开始减弱，锋后低空急流增强，锋后冷平流开始主导锋两侧的环流系统。该冷平流削弱锋两侧的温度水平梯度，产生锋消作用。对这次锋面斜压对流边界层的湍流特征分析表明：在边界层之上切应力wv明显增大；湍能收支分析表明在边界层之上的风切变产生项很强，即大尺度天气系统有利于斜压对流边界层的发展，边界层内各量充分混合。这次冬季锋面暴风雪天气过程，冷锋前的低空南风急流从墨西哥湾携带来的充足水汽及锋区边界层大气的强斜压性是其产生的关键因子：冷锋过后，大尺度高空急流的作用更有利于对流边界层的充分发展。     

离散裂隙渗流方法与裂隙化渗透介质建模

流体渗流模拟的连续介质方法通常适用于多孔地质体，并不一定适用于裂隙岩体，由于裂隙分布及其特征与孔隙差异较大。若流体渗流主要受裂隙的控制，对于一定尺寸的裂隙岩体，多孔介质假设则较难刻划裂隙岩体的渗流特征。离散裂隙渗流方法不但可直接用于模拟裂隙岩体非均质性和各向异性等渗流特征，而且可用其确定所研究的裂隙岩体典型单元体及其水力传导（渗透）张量大小。主要讨论了以下问题：（1）饱和裂隙介质中一般的离散流体渗流模拟；（2）裂隙岩体中的REV（典型单元体）及其水力传导（渗透）张量的确定；（3）利用离散裂隙网络流体渗流模型研究裂隙方向几何参数对水力传导系数和REV的影响；（4）在二维和三维离散裂隙流体渗流模型中对区域大裂隙和局部小裂隙的处理方法。调查结果显示离散裂隙流体渗流数学模型可用来评价不同尺度上的裂隙岩体的水力特征，以及裂隙方向对裂隙化岩体的水力特征有着不可忽视的影响。同时，局部小裂隙、区域大裂隙应当区别对待，以便据其所起的作用及水力特征，建立裂隙化岩体相应的流体渗流模型。     

Vertical dynamic and thermodynamic characteristics of urban lower boundary layer and its relationship with aerosol concentration over Beijing

By utilizing observational data from a 325 m tower of the Institute of Atmospheric Physics (IAP), Chinese Academy of Sciences (CAS) on March 19-29, 2001 and August 11-25, 2003, a comprehensive study was conducted on the vertical dynamical and thermodynamic characteristics of the urban lower boundary layer (ULBL) and its relationship with aerosol concentration over Beijing. Firstly, a comparative analysis was made on the gradient data (wind, temperature and humidity), ultrasonic data (atmospheric turbulences) and air-quality observations at different tower heights (47, 120 and 280 m). Secondly, a diagnosis was made to reveal the major features of normalized variances of velocity and temperature, turbulence kinetic energy as well as their relationship with aerosol concentrations. Furthermore, the characteristics of the ULBL vertical structure and the TSP concentration/distribution variations during a sand/dust weather process were also analyzed. The outcome of the study showed that under unstable stratification, the normalized variances of velocity (σu/u*, σv/u*, σw/u*) and temperature (σT/T*) at 47 and 120 m heights fit the Monin-Obukhov similarity (MOS) framework and the fitting formulas were given out accordingly. According to the stratification parameter (z′/L), the stable ULBL could be divided into 2 zones. With z′/L<0.1, it was a weakly stable zone and MOS framework was applicable. The other was a highly stable zone with z′/L>0.1 and the normalized velocity variances tended to increase along with higher stability, but it remained constant for normalized temperature variances. At daytime, the near-surface layer includes two heights of 47 and 120 m, while 280 m has been above it. The ULBL analysis in conjunction with a sand/dust weather process in Beijing in March 2001 indicated that the maximum concentration of Total Suspended Particulates (TSP) at 320 m reached 913.3 μg/m3 and the particles were transported from the upper to lower ULBL, which was apparently related to the development process of a low-level jet and its concomitant strong sinking motion.     

川西坳陷新场构造带须家河组超压演化与流体的关系

沉积盆地超压体系是油气勘探与开发过程中一个不容忽视的问题,不仅影响了地质流体的运移和聚集,更为勘探带来安全隐患。以实测地压和油田水化学数据为基础,对川西坳陷新场构造带须四段和须二段现今地层水矿化度与现今压力系数关系进行比较,结合地层水特征系数和阴阳离子关系,综合前人研究结果,对须四段和须二段压力演化史与地层水演化过程进行分析,考察川西坳陷须家河组超压系统演化与地层水演化的耦合关系。结果表明,须家河组超压发育与地层水具有密切的联系:(1)须家河组储层段压力分布范围较广,在弱超压至超强压之间,须二段属于中超压而须四段属于超高压,平面分布中须二段探测井中矿化度随压力系数增加而减少,须四段则相反;(2)在压实过程中,由于流体排驱受阻导致"欠压实"超压的产生,随着超压的不断积聚,局部出现裂缝,导致地层水更加强烈的混合作用和运移;(3)生烃作用导致自生压力增大,地层水离子水岩作用强烈,造成流体包裹体与现今地层水离子成分分异;(4)构造挤压抬升过程中,须四段裂缝不发育,压力进一步升高,须二段则产生泄压,出现了凝析水和水侵现象,造成须四段和须二段现今地层水特征的差异。