Fast genetic mapping in barley: case studies of cuticle mutants using RNA-sequencing

Barley(Hordeum vulgare L.) is one of the earliest domesticated crop species and ranked as the fourth largest cereal production worldwide. Forward genetic studies in barley have greatly advanced plant genetics during the last century; however, most genes are identified by the conventional mapping method. Array genotyping and exome-capture sequencing have also been successfully used to target the causal mutation in barley populations, but these techniques are not widely adopted because of associated costs and partly due to the huge genome size of barley. This review summarizes three mapping cases of barley cuticle mutants in our laboratory with the help of RNA-sequencing. The causal mutations have been successfully identified for two of them and the target genes are located in the pericentromeric regions. Detailed information on the mapping-by-sequencing, mapping-and-sequencing, and RNA-sequencing assisted linkage mapping are presented and some limitations and challenges on the mapping assisted by RNA sequencing are also discussed. The alternative and elegant methods presented in this review may greatly accelerate forward genetics of barley mapping, especially for laboratories without large funding.     

流星暴的航天定义

定义ZHR^*以每小时穿过1000平方公里天顶截面的，能在铝质表面打出直径不小于1厘米的弹坑的流星体数，作为流星群对航天安全威胁强度的定量标志，给出了从ZHR到ZHR^*的换算公式．用ZHR^*取代ZHR来评价20世纪90年代以来的一些强流星雨，发现1998年的贾可比尼(天龙座)流星雨对航天安全来说，是比公认的1999、2001、2002年3次狮子座流星暴强数倍的更强流星暴．     

观驾山—黑岛一带信息量法找金研究

本文以成矿地质模型为依据，对观驾山—黑山岛一带的化探、物探、遥感及地质等信息变量进行了详细的分析，选取了１０个信息变量２４个标志状态；在此基础上，运用信息量法对研究区内４５８个单元的信息量值进行计算，并由此确定了研究范围内的成矿远景区。事实表明，研究区信息量成矿预测是非常有效的     

平凉隐伏岩溶水环境同位素研究

地下水资源可持续利用的一个急待解决的重要问题,是对地下水补给和更新能力的评价.利用环境同位素技术研究地下水的补给和可更新性是当前较为新颖的方法之一.在西北干旱、半干旱的隐伏岩溶地区,地下水埋藏条件复杂,常规的地质勘探方法所能提供的水文地质信息有限,环境同位素方法在研究地下水的补给及可更新能力方面发挥了优势,可对传统方法进行补充和验证.其结果表明,研究区隐伏岩溶水形成较早,且有大量现代水的混入,平均混入量为54%.说明区内隐伏岩溶水的补给和更新能力较好.环境同位素分析结果还显示,大岔河隐伏岩溶水为一相对独立、半开放的水文地质单元,其补给来源部分为流域内大气降水、地表水的补给,部分为东南部三道沟岩溶地下水的补给;根据环境同位素EPM模型计算,地下水的滞留时间为36 a.地下水储存量为1.314×108 m3; 储水系数为7.29×10-3.这一结果与传统勘探方法的计算结果基本吻合,说明环境同位素方法的实用性.     

Approach to Mountain Hazards in Tibet, China

Tibet is located at the southwest boundary of China. It is the main body of the Qinghai-Tibet Plateau, the highest and the youngest plateau in the world. Owing to complicated geology, Neo-tectonic movements, geomorphology, climate and plateau environment, various mountain hazards, such as debris flow, flash flood, landslide, collapse, snow avalanche and snow drifts, are widely distributed along the Jinsha River (the upper reaches of the Yangtze River), the Nu River and the Lancang River in the east, and the Yarlungzangbo River, the Pumqu River and the Poiqu River in the south and southeast of Tibet. The distribution area of mountain hazards in Tibet is about 589,000 km^2, 49.3% of its total territory. In comparison to other mountain regions in China, mountain hazards in Tibet break out unexpectedly with tremendously large scale and endanger the traffic lines, cities and towns, farmland, grassland, mountain environment, and make more dangers to the neighboring countries, such as Nepal, India, Myanmar and Bhutan. To mitigate mountain hazards, some suggestions are proposed in this paper, such as strengthening scientific research, enhancing joint studies, hazards mitigation planning, hazards warning and forecasting, controlling the most disastrous hazards and forbidding unreasonable human exploring activities in mountain areas.     

Microwave Type III Burst Group in the 1993 October 2 Flare

Microwave Type III bursts with positive frequency drifting rate were found by Stahli and Benz (1987) for first time. Type III events are especially interesting because they are well-known to be signatures of electron beams in coronal plasma, and they are effective means for diagnosting of source plasma. A microwave burst consisting of some microwave type III burst groups was registered at Beijing Astronomical Observatory with the 2545–2645–2840 MHz synchronous observing system. The distributions of frequency drifting rate, half power duration, and intensity for each impulse in the groups have been statistically analysed. From this analysis, some important parameters for the dynamic process in the flare are deduced and discussed.     

THERMAL EFFECTS OF BUILDING′S EXTERNAL SURFACES IN CITY——Characteristics of Heat Flux into and out of External Wall Surfaces

1INTRODUCTIONWiththerecentmodernizationandurbanizationofChi-na,urbanareashavegreatlyincreased, andgiantbuild-ings,especiallythosetallerthan100m,havebeendomi-nantincities,whichhave resultedinthegreatincreaseintheproportionofbuilding'sexternalsurfacetototalurbanarea.Thedifferencesofthermalpropertiesmain-lyinducedbysolarradiationbetweenbuilding'sexter-nalsurfacesareobviousfordifferentexposures,whichcaninevitablyinfluencethedistributionsofairtempera-turenearby,eventheverticaldistributionsofurb…     

有限元法与伪谱法混合求解弹性波动方程

在地震波场数值模拟中,有限差分法、有限元法和伪谱法都是常用的基本方法,但它们各有不同的适应性和优缺点,如有限差分法、有限元法都存在减弱网格频散和提高计算效率的矛盾,而伪谱法的网格频散小且计算效率高.有限差分法和伪谱法在处理地表结构复杂或地表剧烈起伏以及地下结构复杂的情况时存在较大的难度,而有限元法可较为理想地拟合起伏地表和任意弯曲界面,且可方便地处理自由边界条件和界面边界条件.尝试将有限元法和伪谱法相结合,形成地震波场数值模拟的一种混合方法,利用二者的优点,克服二者的缺点,达到既减弱网格频散又提高计算精度和效率的目的.并采用所谓的‘过度区域‘技术解决两种不同算法的衔接问题.模拟实例表明,给出的混合模拟方法不失为弹性波场数值模拟的一种有效方法.     

利用MODIS红外资料反演大气参数以及表层温度的研究

提出了用牛顿非线性迭代法同时反演大气参数和表层温度,该反演算法应用到我国渤海地区MODIS红外资料中,可反演得到中尺度范围内的大气温度、水汽廓线,其误差分别不超过1.5 K和18%.反演得到的表层温度、大气可降水量(TPW)和大气稳定度(TTI)与美国国家宇航局(NASA)MOD07产品相似.