Investigation of Arsenotrophic Microbiome in Arsenic‐Affected Bangladesh Groundwater

Arsenotrophic bacteria contribute to the nutrient cycling in arsenic (As) affected groundwater. This study employed a culture‐independent and ‐dependent investigation of arsenotrophic microbiomes in As affected groundwater samples collected from Madhabpur, Sonatengra, and Union Porishod in Singair Upazila, Manikganj, Bangladesh. Total As contents, detected by Atomic Absorption Spectrophotometry (AAS) of the samples, were 47 µg/L (Madhabpur, SNGW‐1), 53 µg/L (Sonatengra, SNGW‐2), and 12 µg/L (Union porishod, SNGW‐3), whereas the control well (SNGW‐4; depths >150 m) showed As content of 6 µg/L. Denaturing Gradient Gel Electrophoresis (DGGE) analysis of the amplified 16S rRNA gene from As‐affected groundwater samples revealed the dominance of aerobic bacteria Pseudomonas within heterogeneous bacterial populations. DGGE of heterotrophic enrichments supplemented with arsenite [As (III)] for 4 weeks showed the dominance of Chryseobacterium, Flavobacterium, and Aquabacterium, whereas the dominant genera in that of autotrophic enrichments were Aeromonas, Acinetobacter, and Pseudomonas. Cultured bacteria retrieved from both autotrophic and heterotrophic enrichments were distinguished into nine genotypes belonging to Chryseobacterium, Acinetobacter, Escherichia, Pseudomonas, Stenotrophomonas, Janibacter, Staphylococcus, and Bacillus. They exhibited varying range of As(III) tolerance from 4 to 27 mM. As(III) transformation potential was confirmed within the isolates with oxidation rate as high as 0.143 mM/h for Pseudomonas sp. Sn 28. The arsenotrophic microbiome specifies their potential role in groundwater As‐cycling and their genetic information provide the scientific basis for As‐bioremediation.     

Multi-proxy geoarchaeological study redefines understanding of the paleocoastlines and ancient harbours of Liman Tepe (Iskele, Turkey)

Determining the position of Liman Tepe's (ancient `Clazomenae') archaeological features relative to the coastline is important for understanding their intended function and reconstructing the character of Aegean maritime activities and sea-based trade. Previous attempts at reconstructing harbour locations at Liman Tepe relied on extrapolating paleoenvironments based on modern surface topography. In light of this, samples from a sediment coring survey and terrestrial and underwater archaeological excavations were analysed using multi-proxy geoarchaeological methods to determine paleoenvironmental facies. Micropaleontological (foraminifera), sedimentological (grain-size analysis) and geochemical (δ¹³C/δ¹⁸O) analyses resulted in the reconstruction of the coastal paleogeomorphology, including the presence and absence of ancient harbouring areas. Neither of the previous coastal reconstructions was supported by the new results. Instead, two separate harbouring areas were recognized, one coincident with the Early Bronze Age (4800–3900 years bp) and a second during the archaic and classical periods ( c. 2800–2400 years bp). These results emphasize the necessity for multi-proxy geoarchaeological studies when approaching coastal archaeological sites as a means to reconstruct paleocoastal geomorphology and understand ancient maritime development better .     

Chromium uptake by giant reed under rhizobacterial inhibition

The role of rhizospheric microbes of giant reed (Arundo donax L.) in Cr uptake from hydroponic culture was investigated. The control group was exposed to Cr in range of 25–100 mg L^?1 containing a control itself (with no metal addition). The experimental group received same Cr treatments, but in addition was exposed to antibiotic treatment in order to inhibit rhizospheric bacteria. The range of Cr accumulated in the roots was 3–7.65 mg L^?1; in stem it ranged 2.15–42.4 mg kg^?1; while in leaves, the range of Cr content was 13.7–15 mg kg^?1. Overall, Cr uptake in A. donax (without rhizobacterial inhibition) was root < leaf < stem. However, the amount of Cr uptake in plants with rhizobacterial inhibition was significantly less (~4.6-folds in 100 mg L^?1 Cr treatment) than those without such inhibition clearly highlighting that rhizobacterial inhibition decreased the Cr uptake. The experimental results clearly demonstrated that the inhibition of the rhizobacterial populations had great influence on the Cr uptake. However, Cr uptake could not be completely inhibited as some metal uptake was observed after the rhizobacterial inhibition although it was significantly less than the Cr uptake of plants without such inhibition.     

Macrophytes’ Performance in Floating Treatment Wetlands for the Remediation of Ciprofloxacin-Contaminated Water

The presence of trace antibiotics in domestic and industrial effluents poses a risk of toxicity to fauna and flora. The application of floating treatment wetlands (FTWs) is a low-cost and sustainable approach to remediate wastewater. In this study, the performance of different macrophytes vegetated in FTWs for the remediation of ciprofloxacin (CIP)-contaminated water is investigated. Six macrophytes, Brachia mutica, Typha domingensis, Phragmites australis, Canna indica, Cyperus laevigatus, and Leptochloa fusca, are vegetated in FTWs for the removal of CIP (50 mg L⁻¹) from water. The FTWs show the potential to remove 30–43.58 mg L⁻¹ CIP from water in 28 days. They also reduce the chemical oxygen demand (118–138 mg L⁻¹) and biochemical oxygen demand (35–45 mg L⁻¹) of water. Among the macrophytes, C. indica removes maximum (43.58 mg L⁻¹) and T. domingensis minimum (30 mg L⁻¹) CIP. Canna indica and T. domingensis exhibit the maximum and minimum increase (30% and 12% of dry biomass) in growth, respectively. This study reveals that the FTWs vegetated with different plant species exhibit varying performances in removing CIP from water. This investigation is a step forward toward sustainable bioremediation of water contaminated with antibiotics.     

Riverine flood assessment in Jhang district in connection with ENSO and summer monsoon rainfall over Upper Indus Basin for 2010

Pakistan has experienced severe floods over the past decades due to climate variability. Among all the floods, the flood of 2010 was the worst in history. This study focuses on the assessment of (1) riverine flooding in the district Jhang (where Jhelum and Chenab rivers join, and the district was severely flood affected) and (2) south Asiatic summer monsoon rainfall patterns and anomalies considering the case of 2010 flood in Pakistan. The land use/cover change has been analyzed by using Landsat TM 30 m resolution satellite imageries for supervised classification, and three instances have been compared, i.e., pre-flooding, flooding, and post-flooding. The water flow accumulation, drainage density and pattern, and river catchment areas have been calculated by using Shutter Radar Topography Mission digital elevation model 90 m resolution. The standard deviation of south Asiatic summer monsoon rainfall patterns, anomalies and normal (1979–2008) has been calculated for July, August, and September by using rainfall data set of Era interim (0.75° × 0.75° resolution). El Niño Southern Oscillation has also been considered for its role in prevailing rainfall anomalies during the year 2010 over Upper Indus Basin region. Results show the considerable changing of land cover during the three instances in the Jhang district and water content in the rivers. Abnormal rainfall patterns over Upper Indus Basin region prevailed during summer monsoon months in the year 2010 and 2011. The El Niño (2009–2010) and its rapid phase transition to La Niña (2011–2012) may be the cause of severity and disturbances in rainfall patterns during the year 2010. The Geographical Information System techniques and model based simulated climate data sets have been used in this study which can be helpful in developing a monitoring tool for flood management.