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1.
Nicholas H. Vrolijk Nancy M. Targett Bruce R. Woodin John J. Stegeman 《Marine environmental research》1995,39(1-4)
The relationship between cytochrome P450 and feeding on terpenoid-rich gorgonian corals was investigated in a species of tropical butterflyfish and compared with two other sympatric congeners that do not feed on gorgonians. Fish were collected from non-polluted waters in Belize and the levels of two cytochrome P450 isozymes (CYP2B and CYP3A) were immunoquantitated in addition to quantification of total P450. Chaetodon capistratus regularly feeds on gorgonian corals and has higher levels of total hepatic microsomal cytochrome P450 than C. ocellatus or C. striatus. The content of hepatic P450 (0.588–0.794 nmol mg−1) in C. capistratus is among the highest ever reported in teleosts from non-polluted waters and is significantly greater than detected in C. ocellatus or C. striatus. Chaetodon capistratus also had a larger hepatic index (g liver per g fish) and more microsomal protein (mg protein per g liver), factors that translate into 3.3- to 8-fold more total P450 per g fish. Sexual differences in total P450 were observed between male and female C. capistratus, but not among the other species. The contents of proteins detected by immunoassay with polyclonal anti-scup P450B (CYP2B) and anti-human P4503A (CYP3A) were 2- to 10-fold and 2- to 20-fold greater, respectively, in C. capistratus than in the congeneric species. CYP2 and CYP3 gene families in mammals are thought to have evolved partially in response to dietary allelochemicals. These results suggest that these P450 isozymes may also be important in marine teleosts that feed on terpenoid-rich prey. 相似文献
2.
John J. Stegeman 《Marine environmental research》1992,34(1-4)
A growing number of studies concerning organic chemical pollutant induction of cytochrome P450 mixed function oxidases in fish stimulate a need for common terminology. Similar names for proteins might be based on similarities in function, in regulation, and in their structure. Under the current guidelines for P450 proteins, classification is based on measured or inferred amino acid sequence. At present, a single teleost hydrocarbon-inducible P450 (rainbow trout) can conclusively be termed 1A1: a second (scup P450E) can be so termed on the basis of partial sequence. The lack of primary sequence information makes it difficult to assign a specific identity to hydrocarbon inducible P450s in other fish. Nevertheless, the sum of information on catalytic activities, introduction and immunological cross-reactivities provides a weight of evidence sufficient to assume (but not prove) gene family (I) and subfamily (A) identities for hydrocarbon-inducible proteins in many species. Reference to these proteins as P4501 or IA (or CYPI or IA) in fish species where sequence data are lacking is suggested as more appropriate than reference to them as P450IAI (or CYPIAI). Additional primary sequence data are required to further define orthologous relationships among P450IA genes in fish, and to determine whether IAI is a correct designation for a hydrocarbon-inducible P450 in many species. 相似文献
3.
Organochlorine concentrations were measured in white-sided dolphins, pilot whales, and their prey from the Gulf of Maine and used to identify species, tissue, and gender differences, and trophic transfer trends, in bioaccumulation. Polychlorinated biphenyl concentrations ([PCB]) in dolphin blubber (13 +/- 7.1 micrograms/g fresh wt.) were twice those in pilot whales, but pesticide concentrations (20 +/- 13 micrograms/g fresh) were similar between species. 4,4'-DDE, trans-non-achlor, Cl6(153) and Cl6(138) concentrations were highest. Skin tissues had more recalcitrant organochlorines than the internal organs. Male dolphins bioaccumulated higher concentrations of nonmetabolizable PCBs and hexachlorocyclohexane (HCH) isomers, whereas pilot whales had no gender-related differences in bioaccumulation. Pilot whales, mackerel, and herring had proportionately higher concentrations of DDTs, whereas [PCB] were higher in dolphins and squid. Although these odontocetes feed at the same trophic level and store a similar suite of contaminants, dolphins bioaccumulated higher and potentially hazardous 4,4'-DDE and PCB concentrations from food in their more geographically restricted range. 相似文献
4.
In mammals, the induction of cytochrome P4501A forms by chlorinated dibenzo-p-dioxins, chlorinated dibenzofurans, and halogenated biphenyls is under control of a soluble protein known as the Ah (aromatic hydrocarbon) receptor. Little is known about the presence and properties of the Ah receptor in other vertebrate and invertebrate species. In these studies, we sought evidence for an Ah receptor in the liver or liver-equivalent of 20 species of marine and freshwater animals, using the photoaffinity ligand 2-azido-3-[125I] iodo-7,8-dibromodibenzo-p-dioxin (N3[125I]Br2DD). Specific labeling of cytosolic proteins by N3[125I]Br2DD was observed in seven species of teleost and elasmobranch fish, in PLHC-I fish hepatoma cells, and in beluga whales. No specifically labeled proteins were found in cytosol from two species of agnathan fish nor in any of nine invertebrate species representing eight classes of four phyla. The presence or absence of specifically labeled polypeptides corresponds with the inducibility of cytochrome P4501A and sensitivity to the toxic effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin and related planar halogenated aromatic hydrocarbons in many of these groups. Thus, Ah receptor function may have arisen early invertebrate evolution and has been conserved from elasmobranch and teleost fish to mammals. 相似文献
5.
Coral mortality may result in macroalgal proliferation or a phase shift into an alga-dominated state. Subtidal, high-latitude western Indian Ocean coral communities at Sodwana Bay on the KwaZulu-Natal coast, South Africa, have experienced some mortality because of warm-water anomalies, storms and other causes, but the response of the macroalgae is unknown. We investigated the abundance and diversity of benthic algae on different hard natural substrata (dead digitate, brain and plate corals and beach rock) on Two-Mile Reef, Sodwana Bay. We also compared algal communities colonising ceramic, marble and pretreated ceramic tiles placed on the reef for six months. We identified 95 algae (14 Chlorophyta, 11 Phaeophyceae, 69 Rhodophyta and one cyanobacterium). Assemblages on natural and artificial substrata were dominated by the brown alga Lobophora variegata (Lamouroux) Womersley ex Oliveira and non-geniculate corallines (Rhodophyta, Corallinaceae). Cluster and ordination analyses revealed that the algae showed no affinity for particular substrata, whether natural or artificial. Algal cover was occasionally higher on rougher tiles and crustose corallines were significantly more abundant on marble than ceramic tiles. Two-Mile Reef had 23.1% dead and 48.4% live scleractinian coral cover, where dead corals were colonised indiscriminately by many small algal species, but there was no evidence of algal proliferation. The results provide a baseline for monitoring this high-latitude reef system. 相似文献
6.
辉钼矿Re-Os同位素定年方法的改进与应用 总被引:13,自引:9,他引:4
公认的Re-Os同位素定年代表物辉钼矿,目前已在金属硫化物矿床年代学研究领域获得了广泛的应用。本研究采用浓HNO3分解辉钼矿样品,大大地简化了Re和Os的化学制备过程,并根据辉钼矿中正常Os含量水平相对放射成因187Os可以忽略的特点,以正常Os标准为稀释剂,实现了仪器测量过程中Os同位素质量分馏的在线校正,改善了分析数据的质量。该方法经辉钼矿国家标准物质进行验证,获得了满意的Re、Os含量及Re-Os年龄数据,并且在南岭地区与连阳复式岩体相关的姓坪夕卡岩型钼矿床成矿年龄研究中获得了成功应用。在实际应用中,为了获得有意义的能反映真实地质事件的年龄数据,辉钼矿样品不仅要有足够的取样量,而且还要保证粒度细、混合均匀。 相似文献
7.
Esler D Ballachey BE Trust KA Iverson SA Reed JA Miles AK Henderson JD Woodin BR Stegeman JJ McAdie M Mulcahy DM Wilson BW 《Marine pollution bulletin》2011,62(3):609-614
We examined hepatic EROD activity, as an indicator of CYP1A induction, in Barrow’s goldeneyes captured in areas oiled during the 1989 Exxon Valdez spill and those from nearby unoiled areas. We found that average EROD activity differed between areas during 2005, although the magnitude of the difference was reduced relative to a previous study from 1996/1997, and we found that areas did not differ by 2009. Similarly, we found that the proportion of individuals captured from oiled areas with elevated EROD activity (?2 times unoiled average) declined from 41% in winter 1996/1997 to 10% in 2005 and 15% in 2009. This work adds to a body of literature describing the timelines over which vertebrates were exposed to residual Exxon Valdez oil and indicates that, for Barrow’s goldeneyes in Prince William Sound, exposure persisted for many years with evidence of substantially reduced exposure by 2 decades after the spill. 相似文献
8.
N Loiseau JJ Kiszka T Bouveroux MR Heithaus M Soria P Chabanet 《African Journal of Marine Science》2016,38(1):73-79
Bull sharks Carcharhinus leucas are common along the coast of Reunion Island (South-West Indian Ocean) and were suspected to aggregate in the vicinity of an aquaculture farm in Saint-Paul Bay on the west coast. In order to understand the behaviour and interaction of bull sharks near aquaculture cages at Saint-Paul Bay, we deployed an experimental unbaited stationary video camera. From 175 hours of recording during daylight hours from March to April 2012, eight individual female bull sharks (seven adults and one immature) were identified based on their natural markings. These sharks were resighted between 3 and 45 times. Residency analysis revealed site attachment under the aquaculture cages for at least three individuals over the course of the study. Recorded behaviours included intraspecific social interactions such as synchronised swimming. Social interactions and relatively strong paired associations for two pairs of females suggest some level of sociality among bull sharks around Reunion Island. Overall, our results demonstrate the utility of unbaited video systems to monitor the behaviour of adult coastal sharks. 相似文献
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