Isolation of Protoplasts from Undaria pinnatifida by Alginate Lyase Digestion

1　Introduction　Protoplastsserveasabasicandversatiletoolforge neticengineeringandbiochemicalresearchforseveralreasons :theymayregeneratetheirwallsandprovideamodelsystemforstudyingwallbiogenesis ,theirlysisprovidesagentlemeansofpreparingsubcellularor ganelles ,andthemembraneexpositionallowsgeneticmanipulationsinvolvingfusionoruptakeofnucleicacidstobepossible .Theseadvantagesarelessachiev ablewhenusingintactcells (Liciaetal.,1999) .Incomparisonwithlandplantsandunicellularalgae ,on lylimitedrepo…     

ANALYSIS OF DRIVING FORCES ON THE CHANGE OF HISTORICAL FARMLAND USE IN SHANDONG PROVINCE DURING THE QING DYNASTY (1644-1911)

1INTRODUCTIONTheIGBP-IHDP(InternationalGeosphere-BiosphereProgramme,andInternationalHumanDimensionPro-gramme)jointproject—LUCC(landuse/coverchange)isaninterdisciplinaryprogramdesignedtoimprovetheunderstandingofthedynamicsofland-useandland-coverchangeasinputstoandconsequencesofglobalenvironmentalchangeandsustainabledevelopment(CHARLOTTE,2002).Inparticularthedifferentbio-geochemicalmodeling(e.g.theglobalcarboncycle)ac-tivitiesandcomparativestudies,suchasthoseconduct-edbyIGBP-G…     

CARBON DYNAMICS OF WETLAND IN THE SANJIANG PLAIN

1INTRODUCTIONWetlandsplayanimportant roleintheprocessofcar-bonstorage.Thetotalcarbonstoredindifferentkindsofwetlandsisabout15%-35%ofthetotalcarboninthegloballandsoils(POSTetal.,1982;GORHAM,1991).Inaddition,wetlandsaresignificantnaturalsources fortheatmospheric CH4 (MOORE,1994).It isestimatedthatabout110×1012gCH4 originates fromanaerobicdecompositioninthenaturalwetlands,CH4 emission fromthenaturalwetlandsis15%-30%oftheglobalCH4 emission andtheCH4 emission from thepeat land at hi…     

^226Ra and ^228Ra in the Seawater of the Western Yellow Sea

1Introduction Therearefourradiumisotopesinnature,ofwhich224Ra,226Raand228Rahavebeenwidelyinvestigated inmarineresearch,butthespecificactivityof223Rais toolowtobemeasured,therefore,itsapplicationsarelimited(Moore,1969;Broeckeretal.,1976;KuandLin,1976;Nozak…     

STRATEGY OF MAIZE′S CONCENTRATING TO ADVANTAGE AREAS IN JILIN PROVINCE

WiththedevelopmentofagricultureandChina'sentryintoWTO,thesituationofsupplyanddemandinmaizemarkethaschangedgreatly.Facingtherelativesurplusofmaizeandtheformationofbuyer'smarket,weshouldfullydeveloprelativeadvantages,carryouttheunevenstrategyofsupportingthesuperior inmaizeproduction andcultivateadvantagedareasofmaize.Theabovestrategicmeasurescanrapidlyim-provetheinternationally competitiveabilityandpro-ductivityofmaizeinJilinProvince,getoutofthea-griculturalpredicamentandmaketheagriculturalde-…     

THERMAL EFFECTS OF BUILDING′S EXTERNAL SURFACES IN CITY——Characteristics of Heat Flux into and out of External Wall Surfaces

1INTRODUCTIONWiththerecentmodernizationandurbanizationofChi-na,urbanareashavegreatlyincreased, andgiantbuild-ings,especiallythosetallerthan100m,havebeendomi-nantincities,whichhave resultedinthegreatincreaseintheproportionofbuilding'sexternalsurfacetototalurbanarea.Thedifferencesofthermalpropertiesmain-lyinducedbysolarradiationbetweenbuilding'sexter-nalsurfacesareobviousfordifferentexposures,whichcaninevitablyinfluencethedistributionsofairtempera-turenearby,eventheverticaldistributionsofurb…     

Marine Ingressive Events Recorded in Epicontinental Sequences:Example from the Cretaceous Songliao Basin of NE China in Comparison with the Triassic Central Europe Basin of SW Germany

Songliao Basin is filled predominantly with continental facies sediments including alluvial fan, fluvial plain, fan delta , lacustrine delta, shore ?shallow lacustrine, beach salty flat, semi - deep to deep lacustrine, subaqueous gravity flow, lacustrine swamp and pyroclastic sediments. However, some event units were formed during lake - marine linking periods of the Mid - Cretaceous in the basin, which include black shales with high values for salinity ( Sr/Ba) , alkalinity (Ca Mg)/ (Si Al) , reducibility ( Ni Zn)/Ga and sulfide sulfur as well as heavy isotopes. The Breitenholz ?section to be represen-ted for facies comparison with the Cretaceous evaporitic series in Northeast China is localized in Southwest Germany. Stratig-raphically it belongs to the Crabfeld Formation of Keuper of the Germanic Triassic corresponding to Ladinian - Camian of the international reference scale, and is generally called Lower Gipskeuper. The Germanic Triassic was deposited in the epiconti-nental(cratonic) central Euro     

EMERGY ANALYSIS OF GRAIN PRODUCTION SYSTEM IN JIANGSU AND SHAANXI PROVINCE

1INTRODUCTIONGrain production has always been top-priority issue con-cerned by government and people (MA etal., 2002). Af-ter China put household contract responsibility system in force, its domestic issue of grain was basically settled in the mid 1980s (MA and XU, 1999). However, in the days when idea of sustainable development is broadly accepted, efficiency and sustainability of grain produc-tion are now in question, which have prompted interest in reviewing grain production patterns…     

Tanlu Fault Zone and Gold Ore Metallogenesis

Tanlu Fault Zone (TFZ) is a large NE trending deep fracture system in East China and is about 2500 km long. It extends from the south of Lujiang to Tancheng, and passes through the Bohai Sea and continues to the north, where it branches into Yilan ?Shulan Fault and Dunhua ?Mishan Fault. TFZ is a long lasting huge deep - rooted lithospherical fault system with different characteristics in different parts and at different periods of its evolution. TFZ also controls the distribution and occur-rences of many superlarge, large, middle and small sized gold ore deposits, e. g. Jiapigou, Haigou, Xiaoxihancha and Ciwei-gou gold deposits in Northeast China; Linglong, Jiaojia, Sanshandao, Taishang, Xincheng, Rushan, and Pengjiakuang gold deposits in Jiaodong Peninsula of Shandong Province; and Guilaizhuang and Yinan in Western Shandong Province.     

THE CHARACTERISTIC OF MARINE ENVIRONMENT IN LINGDINGYANG ESTUARY

1 HYDROLOGIC FEATURES Lingdingyang Estuary, located at the middle south of Guangdong Province, is a bell-shaped estuary with a north-south direction. Its area is about 2100km2. The north of Qi′ao Island and Inner-Lingding Island, and the south of Humen are grouped as Neilingdingyang Estuary, having an area of 1041km2. Affected by topography, runoff and tide, its dynamic condition is very complicated. Different water areas have different hydrologic features. The topography under …