Effects of probiotic on microfloral structure of live feed used in larval breeding of turbot Scophthalmus maximus

The effects of an exogenous probiotic( Bacillus amyloliquefaciens) on microbial community structure of B ranchionus plicatils and A rtemia sinica were evaluated in this study during turbot( Scophthalmus maximus) larval breeding. The analysis and comparison of the microfloral composition of live feed with probiotic was conducted using the Illumina HiSeq PE250. The abundance of microbial species and diversity of microflora in live feed with B. amyloliquefaciens were higher than those in the control. The microfloral composition was similar among the three replicate experimental groups of B. plicatils compared with the control after enrichment. L actococcus, Pseudoalteromonas, and Alteromonas were always dominant. Additionally, some other bacterial species became dominant during the enrichment process. The microbial community during nutrient enrichment of A. sinica was rather similar among the three control replicates. Relative abundance of Cobetia sp., the most dominant species, was 54%–65.2%. Similarity in the microbial community was still high after adding B. amyloliquefaciens. Furthermore, P seudoalteromonas and Alteromonas replaced Cobetia as the dominant species, and the abundance of Cobetia decreased to 4.3%–25.3%. Mean common ratios at the operational taxonomic unit level were 50%–60% between the two B. plicatils and A. sinica treatments. Therefore, the microbial community structure changed after adding B. amyloliquefaciens during nutrient enrichment of B. plicatils or A. sinica and tended to stabilize. Additionally, the abundance of V ibrio in any kind of live feed was not significantly dif ferent from that in the control. These results will help improve the microflora of B. plicatils and A. sinica and can be used to understand the multiple-level transfer role of probiotic species among probiotic products, microflora of live feed, and fish larvae.     

基于可靠度理论的黄土高边坡优化设计

对黄土高边坡进行可靠性分析，应用数学原理和优化原理，建立了黄土高边坡的优化模型，对铜黄一级公路黄土高边坡进行分析验证。结果表明，该方法计算的结果与实际较接近．应用中易操作。     

诸广岩体南部基性岩脉的地球化学特征及成因

诸广岩体南部的晚白垩世辉绿玢岩位于西部九峰岩体中部,呈近SN向展布.它具有较高的Al2O3(15.76%～18.70%),K2O(1.97%～3.17%),Na2O(4.20%～4.98%)含量和较低的TiO2(2.76%～2.84%),SiO2(46.75%～50.41%)含量.所有样品Na2O>K2O(Na2O/K2O=1.37～2.13)、大离子亲石元素(Ba,Rb,K,Th)富集明显、Cr和Ni的含量明显低于原始玄武岩浆的参考值;样品中稀土总量较高(158.4×10-6～191.6×10-6),轻稀土富集(LREE/HREE=6.45～6.56),基本无Eu异常(δEu=0.93～0.96).且具有低的εNd(t)值(-0.9～-2.2)、较高的(87Sr/86Sr)I值(0.71276～0.71312)和典型的Dupal异常铅特征.在Nd-Sr和Nd-Pb图解上,辉绿玢岩具有DMM和EMⅡ端员混合特征.这种富集特征不是由地幔岩浆上升过程中与地壳物质之间的同化混染作用造成.而是归因于俯冲带流体对上覆地幔楔的交代改造.因此,诸广岩体南部的辉绿玢岩是在晚白垩世时期地壳伸展和岩石圈减薄的构造环境下,由具有DMM和EMⅡ地幔端员混合特征的富集地幔源区部分熔融形成的.     

西沙群岛海产蓝藻的研究 Ⅴ

本文继续报道我国西沙群岛海产色球藻目 Chroococcales 的十种蓝藻,隶于三科六属，有八种是我国海产蓝藻的新记录,它们是:铜锈微囊藻 Microcystis aeruginosa Kuetzing,小形色球藻 Chroococcus minor ( Kuetzing) Naegeli,湖沼色球藻盐泽变种 C. limneticus Lemm.var. subsalsus Lemm,易变色球藻 C.varius A.Braun,膜状色球藻C. membraninus ( Meneghini) Naegeli,圆胞束球藻 Gomphasphaeria aponina Kuetzing,附钙管鞘藻 Hormathonema epilithicum Ercegovic 和透明拟丝藻 Johannesbaptistia pellucida ( Dickie) Taylor at Drouet。其中透明拟丝藻形态特征独特,这对于蓝藻类的研究,特别是研究从色球藻目进化到丝状蓝藻类的系统演化具有重要价值,早已为藻类学家们所重视。该种也是我国海产蓝藻属的新记录。 迄今为止,藻类学家们一致认为色球藻目是蓝藻门 Cyanophyta 中最原始、最低级的类群,其植物体最简单,大都是单细胞或简单的群体,而且都十分微小,常被肥厚的胶质鞘所包埋,这就使该目蓝藻能适应复杂的自然环境。所以,在地质学和古生物学的研究中它已引起许多学者的注意。有关该目海产种类的分类研究,我国仅有少数报道,这方面的工作还有待深入。     

泉州湾河口湿地鸟类的种类组成与分布

本文根据1988-1999年进行4次泉州湾河口湿地鸟类调查资料，研究了泉州湾河口湿地自然概况，首次报道了该区鸟类13目30科108种，指出泉州湾河口地区鸟类栖息地面临的威胁因素，建议建立泉州湾河口湿地保护区以保护该区的自然生态系统和鸟类资源。     

西沙群岛海产蓝藻类的研究Ⅰ

我国西沙群岛海产蓝藻类的种类极为丰富。但迄今为止除黎尚豪报导过西沙群岛永兴岛的陆生习性的27种蓝藻外,尚未见过任何报告。本文为西沙群岛海产蓝藻类研究的第一部分。根据的材料是中国科学院海洋研究所1975年和1976年在西沙群岛采集的,计127号。本文仅报道11种,隶于颤藻科(Oscillatoriaceae)的6属,其中4种在我国为海产蓝藻的新记录。     

早第三纪济阳湖盆的形成和演变

在构造和气候的综合作用下,本区在早第三纪成为一个大型裂谷湖盆,并在沙四、沙三和沙一期因受古太平洋海泛影响而具有"近海"湖盆的特征,其演变经历了初始形成-强烈扩张-晚期扩张-收缩干涸四个阶段,构成一个完整的湖侵-湖退旋回和湖泊早期形成-中期扩张-晚期收缩三个亚旋回。