Development of a real-time PCR method for Thalassiosira rotula rapid detection

Gene specific primers and DNA probe were designed based on the sequence of 18S rDNA cloned from the red tide alga Thalassiosira rotula. A real-time fluorescent quantitative PCR (RFQ-PCR) method was developed for quantitative detection of T.rotula. The RFQ-PCR assay data showed that the results obtained with the RFQ-PCR quite good agreement with those with the light microscope (LM) counting method, which suggested that the RFQ-PCR could be a useful method for red tide alga detection.     

杂交鲆(牙鲆♀×夏鲆♂)胚胎发育的初步观察

通过人工调控促使亲本牙鲆(Paralichthys olivaceus)♀×夏鲆(Paralichthys dentatus)♂的性腺发育,人工干法授精获得杂交受精卵。杂交受精卵在温度为16.5~17.5℃,光照为100~400 lx,微充气条件下孵化。对杂交鲆胚胎发育全过程进行连续观察并与牙鲆及夏鲆的胚胎发育进行比较。结果表明:杂交鲆卵裂方式与其他大部分硬骨鱼类一样,属于盘状卵裂。受精后经过2 h 30 min进入2细胞期,7 h 45 min进入囊胚期,15 h 10 min进入原肠胚阶段,25 h 30 min进入神经胚期,49 h左右进入尾芽期。授精后63 h左右发现初孵仔鱼,68h左右50%的仔鱼孵出。在杂交鲆的胚胎发育中,主要有2个发育特征与母本类似而与父本不同:视囊的出现要早于体节的分化;孵化前,仔鱼在卵膜内环绕卵黄囊不到1周。但是在其孵化时间上却与父本相似。     

Three-dimensional numerical simulation of oblique wave action on vertical walls

1 Introduction A vertical wall is one of the typical maritime sheltering structures in the coastal region, which is mainly subjected to wave forces. It has been a com- mon engineering assumption adopted for design pur- poses that normally incident wave fo…     

东营凹陷王家岗地区王古1潜山构造特征及油气成藏条件

进一步了解王古1潜山的构造特征及成藏条件,利用渤海湾盆地济阳坳陷东营凹陷王家岗地区的大量三维地震剖面和一些钻井资料,并结合东营凹陷南坡缓坡带区域构造背景及成藏条件分析认为,王古1潜山初始发育于印支期,后经燕山期拉张断陷,切割为断块并抬升,最后于燕山期定型,为典型的缓坡盆倾残丘潜山。该潜山被NW向和NE向断裂切割,潜山高部位侵蚀风化严重,上覆孔店组盖层及沙河街组烃源岩,油气通过断层和不整合面运移至潜山顶部形成侵蚀残丘型油气藏。     

船艇费用-效能分析方法研究

文中介绍了费用-效能分析的基本步骤和方法,针对船艇的特点建立了寿命周期费用的分解结构和费用模型,并编制了船艇寿命周期费用的计算程序。最后以两条船艇的费用-效能分析为例,对费用-效能分析在船艇多方案选优中的应用作了初步探讨。     

A STUDY OF MICROTEKTITES IN THE PACIFIC OCEAN

In 1979, we found many small colorless transparent and light yellow-green glassy spherules in the surface layer and core sediment samples taken from the west of the Central Pacific Occan, which are considered to be microtektites. This finding is worthy of further determination of the range of strewn field and origin of tektites.     

两类双结构矩阵的标准型及其应用

给出2类双结构矩阵的标准型。作为应用,还利用新得出的标准型对这2类双结构矩阵特征值问题发展了结构算法,其中对第一类双结构矩阵特征值问题发展了只用实运算的结构算法。     

Processes Causing the Temporal Changes in Si/N Ratios of Nutrient Consumptions and Export Flux during the Spring Diatom Bloom

Two processes are generally explained as causes of temporal changes in the stoichiometric silicon/nitrogen (Si/N) ratios of sinking particles and of nutrient consumption in the surface water during the spring diatom bloom: (1) physiological changes of diatom under the stress of photosynthesis of diatom and (2) differences of regeneration between silicon and nitrogen. We investigated which process plays an important role in these changes using a one-dimensional ecosystem model that explicitly represents diatom and the other non-silicious phytoplankton. The model was applied to station A7 (41°30′ N, 145°30′ E) in the western North Pacific, where diatom regularly blooms in spring. Model simulations show that the Si/N ratios of the flux exported by the sinking particles at 100 m depth and of nutrient consumptions in the upper 100 m surface water have their maxima at the end of the spring diatom bloom, the values and timings of which are significantly different from each other. Analyses of the model results show that the differences of regeneration between silicon and nitrogen mainly cause the temporal changes of the Si/N ratios. On the other hand, the physiological changes of diatoms under stress can hardly cause these temporal changes, because the effect of the change in the diatom's uptake ratio of silicon to nitrogen is cancelled by that in its sinking rate.     

A Zonal Pathway for NADW in the South Atlantic

Several large deployments of neutrally buoyant floats took place within the Antarctic Intermediate (AAIW), North Atlantic Deep Water (NADW), and the Antarctic Bottom Water (AABW) of the South Atlantic in the 1990s and a number of hydrographic sections were occupied as well. Here we use the spatially and temporally averaged velocities measured by these floats, combined with the hydrographic section data and various estimates of regional current transports from moored current meter arrays, to determine the circulation of the three major subthermocline water masses in a zonal strip across the South Atlantic between the latitudes of 19°S and 30°S. We concentrate on this region because the historical literature suggests that it is where the Deep Western Boundary Current containing NADW bifurcates. In support of this notion, we find that a net of about 5 Sv. of the 15–20 Sv that crosses 19°S does continue zonally eastward at least as far as the Mid-Atlantic Ridge. Once across the ridge it takes a circuit to the north along the ridge flanks before returning to the south in the eastern half of the Angola Basin. The data suggest that the NADW then continues on into the Indian Ocean. This scheme is discussed in the context of distributions of dissolved oxygen, silicate and salinity. In spite of the many float-years of data that were collected in the region a surprising result is that their impact on the computed solutions is quite modest. Although the focus is on the NADW we also discuss the circulation for the AAIW and AABW layers.     

The fate of dumped sediments monitored by a high-resolution multibeam echosounder system,Weser Estuary,German Bight

With the development of high-resolution multibeam echosounder systems (MBES) for surveying shallow-water areas a new tool is available to monitor rapid changes in seabed morphology as, e.g., caused by the dumping of dredge spoil in coastal waters. In this study, four data sets of repeated bathymetric surveys with a MBES were processed and analyzed. The data were collected in a 1.94-km² dumping site in the outer Weser Estuary (German Bight). Between June and December 1998, 2.6 million m³ of dredged sediment were deposited there. The bathymetric maps generated in the course of this study reveal features such as subaqueous dunes, scour holes, and mounds of dumped dredge spoil. The mean water depth decreased by about 1 m during the dumping period. Furthermore, difference grids showing changes in sediment volume allowed a calculation of the sediment budget for the monitored area. After a time period of only 5 months, 0.5 million m³ of the originally dumped 2.6 million m³ of dredge spoil had already been removed from the dumping site.