Molecular phylogenetic analysis of attached Ulvaceae species and free-floating Enteromorpha from Qingdao coasts in 2007

Based on the sequence data of the nuclear ribosomal DNA internal transcribed spacer(ITS) 1,5.8 S,and ITS 2,the molecular phylogeny was analyzed on Ulvaceae species collected from Qingdao coasts in summer of 2007,including 15 attached Ulva and Enteromorpha samples from 10 locations and 10 free-floating Enteromorpha samples from seven locations.The result supported the monophyly of all free-floating Enteromorpha samples,implying the unialgal composition of the free-floating Enteromorpha,and the attached Ulvaceae species from Qingdao coasts were grouped into other five clades,suggesting that they were not the biogeographic origin of the free-floating Enteromorpha in that season.     

不同光生物反应器培养坛紫菜 丝状体的比较研究

Vegetative cells of Porphyra haitanensis sporophytes were grown in a bubble-column and a draft-tube internal-loop airlift bioreactor at 20℃ with an illuminance of 40μmol photons m-2 s-1 for 20days. The draft-tube internal-loop airlift bioreactor yielded maximum cell density of 4850mg DCW (Dried Cell Weight)/L and higher growth rate of 0.094/d, compared to the bubble-column, 3950mg DCW/L and 0.086/d, respectively. The advantages were related to the more homogeneous fluid dynamic characteristics in the draft-tube internal-loop airlift bioreactor than the bubble-column bioreactor.     

耕地开垦费如何计算——浅析征地区片综合地价实施后河南省耕地开垦费计征办法

“十五”期间,全国因建设占用、灾毁、生态退耕、农业结构调整共减少耕地11380万亩,同期土地整理复垦开发补充耕地2140万亩,耕地面积净减少9240万亩.截至2005年10月31日,全国耕地面积18.31亿亩,人均耕地1.4亩.     

重磁异常正反演计算在煤田中的应用

以海拉尔盆地呼和湖研究区为例,对重磁异常进行正、反演计算,求取各个主要地层组、段顶界面埋深。Ｐａｒｋｅｒ正、反演计算方法,兼有频率域里计算速度快,空间域计算准确的优点。该方法是重磁异常定量解释中有利工具,在平面解释中各层深度值连续性加强,适于煤田地质、油田地质普查阶段、高精度重磁勘探,定量解释、为后期钻探提供参考数据。     

裙带菜配子体基因工程选择标记的研究

研究了裙带菜(Undaria pinnatifida)配子体对除草剂草丁膦的敏感性以及温度对草丁膦处理效果的影响。结果表明,裙带菜配子体对草丁膦敏感;统计学分析得出了草丁膦在不同温度条件下处理裙带菜配子体的半致死浓度(LD50)及95%可信限;温差达到10℃时,相同处理时间各组内不同处理温度样品的LD50之间呈现显著差异,第6天的LD50由10℃组的15.78 mg/L降为20℃组的11.56 mg/L和25℃组的10.33 mg/L。研究发现可以尝试采用草丁膦作为裙带菜配子体遗传转化的选择试剂。     

一次超级单体的多普勒雷达特征分析

运用长沙多普勒天气雷达的基本反射率因子(R)、径向速度(V)、垂直积分液态含水量(VIL)、垂直剖面等产品,分析了2008年4月8日发生在湘东地区的一次超级单体强对流天气过程。分析表明:这次过程是一次典型的超级单体过程,在反射率因子上出现了钩状回波、三体散射回波、弱回波、高悬垂回波,相应的径向速度图上出现了持久的中气旋,风暴顶表现为强烈的辐散,降雹前相应的液态垂直累积含水量有一个跃增,这些多普勒雷达特征均为超级单体风暴预警提供了有利的临近预报因子。     

GAMIT在虚拟机系统中的安装与使用

GAMIT／GLOBK软件是全球应用最广泛的GPS数据分析软件之一,运行于Linux操作系统。由于Linux版本众多,使得该软件的安装过程中遇到很多的问题。介绍了以RedHat9．0虚拟机系统平台,安装、使用最新版GAMIT／GLoBK10．40的详细过程,并以实例进行了检验。     

盆地烃类运移二维二相流动数学模型分析与应用实例

从地质固体系统与流体系统相互作用所形成的能量流、物质流的观点，系统地论述了建立烃类运移主要控制方程，即烃水两相拉格朗日型异常地层压力方程和饱和度方程，研制了开发了烃类运移数值模拟系统，阐明了该耦合系统的要素功能和求解方法，提供了改善系统模拟精度的措施，最后介绍了该模拟系统在松辽盆地东南隆起某测线的应用实例。     

Molecular phylogenetic analysis of attached Ulvaceae species and free-floating <Emphasis Type="Italic">Enteromorpha</Emphasis> from Qingdao coasts in 2007

Based on the sequence data of the nuclear ribosomal DNA internal transcribed spacer (ITS) 1, 5.8 S, and ITS 2, the molecular phylogeny was analyzed on Ulvaceae species collected from Qingdao coasts in summer of 2007, including 15 attached Ulva and Enteromorpha samples from 10 locations and 10 free-floating Enteromorpha samples from seven locations. The result supported the monophyly of all free-floating Enteromorpha samples, implying the unialgal composition of the free-floating Enteromorpha, and the attached Ulvaceae species from Qingdao coasts were grouped into other five clades, suggesting that they were not the biogeographic origin of the free-floating Enteromorpha in that season. Supported by NSFC (40506030); the Innovative Key Project of the Chinese Academy of Sciences (KZCX2-YW-209); Science & Technology Project of Qingdao City (06-2-2-12-JCH)     

鲜浒苔稀硫酸水解产糖工艺研究

分别用质量分数为0.6%、1.0%、1.4%、1.8%、2.2%的稀硫酸在121℃高温高压的条件下水解鲜浒苔30、60、90 min,浒苔水解残留物再用纤维素酶酶解,用DNS法测定上述各步骤中的还原糖产量。结果表明,浒苔总糖含量为67.2%。稀硫酸水解工艺中,硫酸质量分数为1.8%、水解90 min,浒苔还原糖转化率最高,达59.9%,每克干质量浒苔可产生447.0 mg还原糖。稀硫酸水解残留物纤维素酶酶解工艺中,浒苔还原糖转化率较低,在硫酸质量分数为0.6%、水解时间为60 min时还原糖转化率出现最大值,仅为6.5%。结果显示浒苔稀硫酸水解产还原糖效果显著,但并不能促进纤维素酶酶解。浒苔糖化可单独采用稀硫酸水解工艺,本实验条件下的最佳工艺条件为1.8%硫酸水解90 min。本研究探索了浒苔糖化工艺条件,对后续的浒苔燃料乙醇发酵研究具有一定的参考意义。