Concurrent imaging of chlorophyll fluorescence,Chlorophyll a content and green fluorescent proteins‐like proteins of symbiotic cnidarians

Research on photosynthetic cnidarians has been mainly focused on the symbiosis established between the cnidarian host and its dinoflagellates endosymbionts from genus Symbiodinium. Despite the potential of imaging techniques for assessing the spatial distribution of key parameters of cnidarian photobiology, such as photochemical activity, chlorophyll a content or green fluorescent proteins (GFPs), to our best knowledge, no study has ever attempted to simultaneous map these three features. In this study, we developed a modified imaging pulse amplitude fluorometer by applying excitation light of different wavelengths and selectively detecting short spectral bands through bandpass filters. The imaging system was used to sequentially excite and quantify chlorophyll variable fluorescence (maximum quantum yield of photosystem II, F_v/F_m), Chl a content (normalized difference vegetation index) and relative content of GFPs. The spatial distribution of these photophysiological parameters was mapped both horizontally, across the surface of the soft corals Sarcophyton cf. glaucum and Sinularia flexibilis and the zoanthid Protopalythoa sp., and vertically, throughout a vertical section of S. cf. glaucum. Results showed bleached areas within each individual coral colony and registered photophysiological changes with S. cf. glaucum tissue depth. Analysis of Protopalythoa sp. polyps’ expansion revealed differential surface patterns of NDVI and GFP concentration, and a negative relation between these latter parameters within each polyp. This novel non‐invasive approach allowed a high‐resolution characterization of the spatial relationship between these key parameters through the analysis of image information on a pixel‐by‐pixel basis, which has great potential for investigating the physiological state of symbiotic associations.     

站式全景遥感影像展示方法与应用技术研究

虚拟漫游技术已成功应用到社会各个领域,传统漫游技术如VRML(Virtual Reality Modeling Language)、Quick Time、PTGui(Panorama Tools)等存在集成难度大、建模时间长、投入成本高等问题。本文基于全景相机以及Pano2vr、Dynamic-Photo HDR、Photoshop等软硬件环境,研发搭建了一整套旅游景区全景影像采集与展示平台。该平台具有良好的沉浸性、多视角浏览、实现方法简单、搭建快速等优点,为旅游景点、酒店、历史文化名胜等场景的360度实景展示提供了有效地工程化解决途径。     

全景深度图像精度分析及优化方法研究

针对三维激光点云数据生成360°全景深度图像存在像素分辨率不均匀的问题,提出一种顾及目标量测精度及可见度的全景深度图像生成方法,在保证全景影像表达地物的完整性的前提下提高其数据的存取精度。通过坐标转换和投影变换生成与全景影像匹配的全景深度图像;基于摄影成像原理分析摄影中心高度、深度值和像素分辨率之间的关系,得到不同深度处目标分辨率随摄影中心升高趋于一致的结论;综合分析地面目标分辨率和杆状目标尤其是树冠对树干的遮挡问题,确定特定场景下生成全景深度图像的最佳摄影中心位置并重新生成深度图像。实验分析表明,该方法能够在保证杆目标可见度的前提下提高地面目标量测精度。     

一类信号恢复问题的投影迭代硬阈值算法

本文给出了基于L₀模求解该问题的非凸模型,借助于稀疏正则化方法来克服问题的不适定性。该模型利用紧小波框架对信号进行稀疏逼近,并利用L₀模度量稀疏性。提出了求解该模型的投影迭代硬阈值算法,并证明了算法的全局收敛性。该算法每一步都有闭式解,计算过程简洁高效。数值实验表明,方法在重建信号的视觉质量和量化指标方面均优于所对比的pFISTA方法。     

基于MODIS的春、夏季中国近海气溶胶对于云参数的影响差异研究

通过分析中国近海MODIS数据中气溶胶参数与云参数的相互关系,讨论了该区域气溶胶的间接效应及其对于云的可能影响.结果表明,在中国近海,气溶胶具有明显的间接效应,而且由于气溶胶种类和水汽的季节变化,使得气溶胶的间接作用具有很强的时间变化特征.在夏季,由于人为气溶胶占主导,它作为有效的云凝结核,使得气溶胶光学厚度（AOT）分别与云凝结核数（CCN）有正相关、与云滴有效半径（CER）有负相关性、与云光学厚度（COT）也存在着正相关,气溶胶的间接效应明显;在春季,由于沙尘气溶胶盛行,同时沙尘并不是很好的云凝结核,使得气溶胶光学厚度（AOT）分别与云凝结核数（CCN）的正相关减弱、与云滴有效半径（CER）则由夏季的负相关变为正相关、与云光学厚度（COT）存在弱的负相关,气溶胶的间接效应不明显.     

光学显微镜成像方式及投影冷光源的分频合成探讨

传统显微镜光源主要包括卤素灯光源、纯水过滤热射线光源、LED光源等,由于卤素灯光源含有大量760~1400 nm区段的红外线,纯水过滤热射线光源滤除红外线不彻底且水会导致透射光线的部分散射,LED光源在500 nm波长附近的光照强度不足、波形存在缺陷,容易灼伤样品或使成像质量受限,都难以满足需要采用大功率冷光源进行无损、高质量显微观察成像和测试的要求。本文在总结光学显微镜的成像方式、光源类型及各类光源优缺点的基础上,提出了采用三棱镜-凸透镜组分频合成冷光源的新思路。该思路首先利用三棱镜将卤素灯光源光谱按频率顺序打开,然后选择可见光区域内的光谱,用凸透镜组校正可见光方向后,再由三棱镜将这些可见光合成不含热射线的冷光源。理论和砂岩荧光薄片实际测试效果表明,该方法既可完全排除热射线,从而使样品免遭受破坏,又保持了完整的可见光光谱,解决了大功率冷光源红外线生热和光谱存在缺陷的难题,这为采用大功率冷光源的光学显微镜投影高质量成像提供了新的方法及切实可行的依据。该套分频合成光源的装置主要配置为:玻璃材质的同规格的三棱镜与凸透镜各两个,卤素灯光源一个。结构简单,可在需使用大功率冷光源进行无损成像和测试等方面获得广泛的应用。     

The symbiotic star CH Cygni – I. Non-thermal bipolar jets

Very Large Array surface brightness and spectral index maps of the evolving extended emission of the triple symbiotic star CH Cygni are presented. These are derived from observations at 4.8, 8.4 and 14 GHz between 1985 and 1999. The maps are dominated by thermal emission around the central bright peak of the nebula, but we also find unambiguous non-thermal emission associated with the extended regions. Our observations confirm that this is a jet. The central region has been associated with the stellar components through Hubble Space Telescope imaging. If the jets are the result of ejection events at outburst, expansion velocities are consistent with those from other measurement methods. We propose that the non-thermal emission is caused by material ejected in the bipolar jets interacting with the circumstellar wind envelope. The resulting shocks lead to local enhancements in the magnetic field from the compact component of the order of 3 mG.