热稳定κ-卡拉胶酶产生菌Bacillus sp. Car19的发酵条件优化

对一株分泌热稳定κ-卡拉胶酶印尼热泉菌进行了种属鉴定, 并采用响应面法对该菌发酵产酶条件进行了优化。鉴定结果表明, 该菌株属于芽孢杆菌属(Bacillus), 命名为Bacillus sp. Car19(GeneBank: KT865196)。发酵条件优化结果显示, 9个环境因子影响Bacillus sp. Car19产酶量。其中影响Bacillus sp. Car19产酶量的三个主要因素分别为培养温度、培养基中Cu²⁺浓度和培养基中NaCl浓度。综合次要因素对Bacillus sp. Car19产酶影响, Bacillus sp. Car19最佳产酶发酵条件为: 培养温度52.31℃、Cu²⁺浓度6.93 mmol/L、NaCl浓度37.03 g/L, 培养基pH为6, 接种量1%, 培养时间36h, 半乳糖浓度0.3 g/L,硝酸铵浓度7g/L, 卡拉胶浓度0.5g/L。优化后发酵上清液酶活力达到15.21 U/mL, 与优化前相比提高了1.5 倍。     

基于SMOS卫星数据的海表面盐度模型

海表面盐度是研究海洋对全球气候影响以及大洋环流的重要参量之一,而卫星遥感技术是获取海表面盐度数据的最有效方法.目前,L波段的SMOS和Aquarius/SAC-D遥感卫星正在用于探测海表面盐度,并根据卫星观测数据和物理机制反演出海表面盐度的产品.但在某些近陆地区域,由于淡水流入及陆地射频(RFI)等因素影响,卫星反演盐度的产品精度较低.文中利用“东方红2号”科学考察船的实测数据、SMOS卫星数据,首次针对中国南海海域提出了用贝叶斯网络模型计算海表面盐度,并用验证数据集(实测Argo盐度)对模型进行适应性评估.经过计算,模型误差和验证误差分别为0.47 psu和0.45 psu,而相应的SMOS Level 2产品的精度分别为1.90 psu和1.82 psu.此模型为海表面盐度的计算提供了一个新方法.     

锈蚀钢筋在疲劳荷载作用下压磁场分布研究

针对锈蚀钢筋的疲劳损伤与寿命评估中的难度大、精度低等问题,引入压磁检测技术,对锈蚀钢筋疲劳过程中的压磁场分布进行检测,从宏观与微观相结合的角度分析压磁场分布与疲劳损伤之间的联系。试验结果表明,锈蚀钢筋的压磁场分布能够反映疲劳损伤的演变规律,疲劳后期非线性特征加剧,锈蚀率及应力幅越大则变化越为明显,磁感强度值、磁场分布梯度、"反转"现象等均有助于进行疲劳损伤评价与寿命预测。研究结果可为压磁无损检测技术在钢筋混凝土结构中的应用提供依据。     

Bias estimation and correction for triangle-based surface area calculations

The calculation of surface area is meaningful for a variety of space-filling phenomena, e.g., the packing of plants or animals within an area of land. With Digital Elevation Model (DEM) data we can calculate the surface area by using a continuous surface model, such as by the Triangulated Irregular Network (TIN). However, just as the triangle-based surface area discussed in this paper, the surface area is generally biased because it is a nonlinear mapping about the DEM data which contain measurement errors. To reduce the bias in the surface area, we propose a second-order bias correction by applying nonlinear error propagation to the triangle-based surface area. This process reveals that the random errors in the DEM data result in a bias in the triangle-based surface area while the systematic errors in the DEM data can be reduced by using the height differences. The bias is theoretically given by a probability integral which can be approximated by numerical approaches including the numerical integral and the Monte Carlo method; but these approaches need a theoretical distribution assumption about the DEM measurement errors, and have a very high computational cost. In most cases, we only have variance information on the measurement errors; thus, a bias estimation based on nonlinear error propagation is proposed. Based on the second-order bias estimation proposed, the variance of the surface area can be improved immediately by removing the bias from the original variance estimation. The main results are verified by the Monte Carlo method and by the numerical integral. They show that an unbiased surface area can be obtained by removing the proposed bias estimation from the triangle-based surface area originally calculated from the DEM data.     

Opportunistic natural experiments using digital telemetry: a transit disruption case study

ABSTRACT

In the past decade society has entered a technological period characterized by handheld computing that supports input and processing from numerous sensors. Today’s mobile phones offer the ability to integrate input from sensors monitoring various external and internal sources (e.g., accelerometer, magnetometer, microphone, GPS, wireless Internet, and Bluetooth). Furthermore, these raw inputs can be integrated and processed in ways that can offer novel representations of human behaviour. As a result, new opportunities to examine and better understand human spatial behaviour are available; one such application is the constant monitoring of a group of people over an extended period of time. Such a research setting lends itself to natural experiments that emerge as a result of regular and on-going observations. We report here on the observation of a natural experiment that took place in the context of a month-long monitoring study of 28 participants using mobile phone-based ubiquitous sensor monitoring. The implications for public health and transportation planning are discussed.     

桩锚支护深基坑施工过程变形特征分析

以济南市某大型深基坑工程为依托,通过FLAC3D对深基坑工程不同施工阶段的变形特性进行了数值计算分析;桩锚支护深基坑,地表最大竖向变行产生在距离基坑边缘10 m位置处,且最大竖向变形量为46.16 mm;最大水平位移发生在桩顶冠梁位置处,最大水平位移为53.45 mm,是基坑支护最薄弱环节,基坑呈现三角形状向内侧滑动的趋势。     

山东曹县地区土壤地球化学特征

山东省曹县地区土壤地球化学调查是按浅层土壤样品1点/4 km~2,深层土壤样品1点/16 km~2。测试N,P,K,Cu,Zn,B,Mo,Mn,F,As,Hg,Pb,Cr,Cd等14种指标。在统计这些指标的表层、深层土壤地球化学特征参数的基础上,对其与世界、全省同类参数的差异进行了分析,并分析了区内表层、深层土壤元素含量的相关性,认为该区表层土壤元素含量的显著特征是高F而低P,Zn,Mo。大部分元素在表层土壤中的含量继承了土壤母质的成分特征,但N,P,F,Hg等受人为活动和污染源的作用在表层土壤中明显富集。     

Diagnosis of earth-fill dams by synthesised approach of sounding and surface wave method

The spatial distribution of the strength inside the earth-fill is identified by the sounding tests. In this research, the Swedish weight sounding (SWS) is employed, and the spatial high-density test is performed to identify the spatial correlation structure. Furthermore, the synthesised approach of the SWS and surface wave method, which is one of the geophysical method, is proposed to compensate the shortage of each approach. Consequently, the correlation structure of an earth-fill could be identified accurately, and the high resolution of the spatial distribution could be visualised based on the survey results.     

Effects of linear and exponential heterogeneity on the dynamic response of a moving load in an irregular isotropic half-space: a comparative study

Heterogeneity, being a trivial feature inside the earth or in a geostructure, makes a strong basis for its consideration in the study of geomechanics. Inclusion of the concept of heterogeneity along with irregularity in the medium brings a novelty to the existing literature related to the study of the moving load. The present study investigates the effects of linear and exponential heterogeneity on the dynamic response due to a normal load moving with constant velocity on a rough irregular heterogeneous isotropic half-space in a comparative approach. Expressions for both normal and shear stresses for either case of heterogeneity have been established in closed form. Substantial effects of the affecting parameters such as depth, irregularity factor, maximum depth of irregularity, frictional coefficient, linear heterogeneity parameter and exponential heterogeneity parameter on normal and shear stresses for both the cases of heterogeneity have been observed. Numerical computation has been carried out and the effects of said parameters have been meticulously examined by means of graphs. Moreover, different cases of heterogeneity and homogeneity along with various types of irregularity namely rectangular, parabolic and no irregularity are compared which serve as a focal theme of the study.     

Effects of Mudstone Particle Content on Shear Strength of a Crushed Sandstone–Mudstone Particle Mixture

The present study focuses on the shear strength, deformation, and particle crushing of sand which is mixed by crushed sandstone and mudstone particles. Two triaxial tests—one for unsaturated specimens and the other for saturated specimens—were performed, along with sieve analysis tests. Results obtained from the triaxial tests indicate that, with the increment of the mudstone particle content by weight, the angle of internal friction decreases, the cohesion increases and then decreases, and both the initial angle of shearing resistance at the atmospheric pressure and decremental angle increase and then decrease. Compared to the unsaturated specimens, the deviator stress or normalized deviator stress of the saturated specimens at the same axial stress may decrease due to the wetting action. Thus, the shear strength of the tested materials may be reduced by the wetting action. Results of sieve analysis tests indicate that the mixing of some mudstone particles into the sandstone particle mixture may reduce the amount of particle crushing, but the wetting action may increase the amount. The amount of the crushed particle may arrive at a minimum of 40% of mudstone particle content by weight.