Inorganic and organic nitrogen cycling in the Southern California Bight

On the basis of mass balance calculations performed for nitrogen (N) uptake experiments in the Southern California Bight (SCB), it has been suggested that a significant portion of dissolved inorganic N (DIN) uptake results in the production of dissolved organic N (DON). To investigate this process, the fate of ammonium (NH₄⁺) and nitrate (NO₃⁻) uptake was quantified within the euphotic zone at three coastal stations in the SCB using ¹⁵N tracer techniques. Several trends in the fate of DIN and the production of DON were observed. First, production of particulate N (PN), from both NH₄⁺ and NO₃⁻, was quantitatively more important in near surface waters, while DON release dominated within the nitracline. Second, the percentage of gross N uptake released as DON was generally higher when NO₃⁻, rather than NH₄⁺, was the substrate. Third, the percentage of N released as DON was higher at night, relative to the day. Fourth, rates of DON release were significantly correlated to NH₄⁺ regeneration, suggesting that similar mechanisms are responsible for both processes—presumably grazing. The results of this study indicate that the DON pool is a sink for DIN uptake on the time scale of hours. One implication of this finding is that new production estimates based on ¹⁵NO₃⁻ uptake rates will likely underestimate particle flux out of the surface layer because the rate of NO₃⁻ uptake is underestimated due to loss of DO¹⁵N during the incubation. On time scales of months to years, however, the N that is taken up as NO₃⁻ and released as DON will likely contribute to export flux via incorporation of the dissolved phase during seasonal mixing into sinking particles or transport. The export of DON on these time scales argues for the use of gross uptake rates to calculate f-ratios.     

五种中草药对海洋光合细菌生长影响的研究

研究了不同方式提取的中草药浸提液以及不同添加量的浸提液对海洋先合知菌生长的影响。结果表明对甘草、黄芩、栝楼等中草药水煮法效果较好,而对青蒿、党参等中草药醇提法效果较好,同时发现上述几种中草药对海洋光合细菌的生长均有促进作用。其中添加4.0%,4.0%和1.0%的栝楼、黄苓和党参浸提液的细菌经过120h的培养.细菌数分别比对照组提高了99.1%、68.8%和68.4%,甘草,青蒿.比对照组提高了10.9%和6.9%,其最佳添加量均为0.5%。     

Respiration partitioning in contrasting subtidal sediments: seasonality and response to a spring phytoplankton deposition

Biomass and respiration rates of bacteria, nematodes and macrobenthos were estimated in relation to the deposition of the spring phytoplankton bloom at two contrasting sites in the Southern North Sea: one with fine‐grained sediment close to the coastline and another with highly permeable sediments. Sediment community oxygen consumption (SCOC) was also measured. Bacterial biomass was relatively similar at both stations, whereas nematode and macrobenthic biomass were higher in fine‐grained sediment. In fine sediments, bacterial biomass increased quickly after deposition of the phytoplankton bloom, whereas the response of nematodes and macrobenthos was delayed. In coarser sediments, nematodes and macrobenthos also showed a fast response in terms of density and biomass. Respiration in permeable sediments was mainly dominated by bacteria at all periods of the year. Hence, nematode and macrobenthic respiration did not contribute strongly to SCOC. This is in contrast to the patterns observed in finer sediments, where both macrofauna and nematodes were important oxygen consumers as well. Macrobenthos contributed more to total SCOC than did nematodes in winter. However, shortly after the arrival of phytodetritus at the sea floor, nematodes and macrobenthos contributed equally to the total SCOC, indicating that all benthic size classes should be taken into account when investigating marine benthic respiration rates.     

黑潮源区沉积物微生物多样性初步研究

从黑潮源区采集上层沉积物,进行DNA提取,以细菌和古菌的16S rDNA通用引物PCR扩增黑潮源区沉积物中细菌和古菌群落的16S rDNA,并构建细菌和古菌的16S rDNA文库,经限制性片段长度多态性分析(Restriction Fragment Length Polymorphism,RFLP),DNA序列测定和系统发育分析,对黑潮源区表层沉积物的细菌和古菌多样性进行了研究。研究结果表明:黑潮源区细菌包括了变形杆菌(Proteobacteria)、酸杆菌(Acidbacterium)、浮霉菌(Planctanycene)、疣微菌(Verrucomicro-bia)和Candidate division OP8和拟杆菌(Bacteroidetes)共6个类群,其中变形杆菌是优势类群。古菌包括了泉古菌(Crenarchaeota)和广古菌(Euryarchaeota),其中泉古菌占优势;泉古菌包括MCG、C3、MBGA和MGI 4个类群,而广古菌包括SAGMEG、MBGE和MEG 3个类群,其中MBGE是优势类群。     

Ecological Effects of Remote Smallsand Descending on Urban Area:Deduced from the "Mud - Snow" in Changchun City

IntroductionContinued sandstorms occurring in North Chinaeally this year have attracted close attention of manyscientists from different aspects. It is recorded thatserious sandstorms occurred eight times in China in1960' s, thirteen times in 1970, s, fou…     

Microbial response to surface microtopography: the role of metabolism in localized mineral dissolution

We examine the role of microtopographical surface features on sulfide minerals in localizing and aligning bacterial adhesion. Experimental data shows strong correlation between bacterial cell alignment and principal crystallographic axes of pyrite (100 and 110). While bacteria often adhere to visible surface imperfections such as scratches, in many cases no associated surface features are visible. Additionally, the size of the surface imperfection does not unambiguously determine its effect in localizing and aligning bacterial cells. We theoretically model bacterial adhesion. We find that the depth of a surface feature such as a scratch is less important than its cross-sectional shape. Surface features that conform to the bacterial shape can strongly alter local bacterial adhesion energies, even with heights of only 10 nm. Hence, small local surface alterations due to bacterial metabolism could strongly affect local adhesion parameters, and may account for the observed bacterial distributions on mineral surfaces.     

Reduzierung von Bakterien- und Coliphagen-Konzentrationen auf der Fließstrecke eines Klärwerksableiters

Reduction of the Concentration of Bacteria and Coliphages along the Flowing Stretch of a Treated Sewage Channel The efficiency of surface waters to eliminate E. coli, fecal streptococci, Salmonella spp., and coliphages was evaluated in a small river which receives treated wastewater and which is rich in submerged macrophytes. The study took place between April and December, 1994. Total colony count, BOD₅, O₂ concentration and water temperature were determined in the river as well. As the river does not receive additional water downwards along its 17.2 km course, dilution effects could be ruled out as the cause for the elimination of the microorganisms. The reduction is assumed to happen rather due to sedimentation, grazing, and adsorption to the submerged waterplants. Immediately after discharge of the wastewater, the river water contained about 10⁵ cfu/100 mL E. coli and 10⁴ cfu/100 mL fecal streptococci, about 1000 pfu/100 mL coliphages, and, as a rule, was positive for salmonella in 10 mL. The reduction of E. coli, fecal streptococci, salmonella, clostridia, and coliphages at the end of the course was 1 to 2 orders of magnitude. This reduction took place mainly within the first 4.7 km, a part in which, due to low flowing velocities, suspended solids settle down efficiently. Besides, at the end of this part the submerged waterplants are especially abundant. The reduction of suspended solids correlated positively with that of BOD₅, bacteria, and coliphages. The reduction of microorganisms was not sufficient to fulfill the requirements of the European Community guidelines for bathing waters and for surface waters used as drinking water source. The regenerating capacity of surface waters is not sufficient to eliminate pathogens from convenionally treated wastewater. Therefore, tertiary treatment is necessary to keep receiving waters reasonably free from pathogens.     

紫金山铜矿原地破碎细菌浸出工艺的可行性分析

根据紫金山铜矿的实际情况，分析采用原地破碎细菌浸出工艺回收紫金山铜矿资源的工程技术可行性，在此基础上进行经济方面的讨论。     

The effect of distribution of iron-oxyhydroxide grain coatings on the transport of bacterial cells in porous media

 Among the demonstrated processes influencing the transport of bacteria through aquifers, the deposition of cells on mineral surfaces is one of the most important. For example, understanding the transport of introduced bacteria through aquifers is essential to designing some in situ bioremediation schemes. The impact of the presence and distribution of Fe(III)-oxyhydroxide-coated sand grains on bacterial transport through porous media was evaluated in column experiments in which bacteria (short rods; 1.2 μm length) were eluted through columns of quartz sand (0.5–0.6 mm in diameter) for several conditions of chemical heterogeneity of mineral substrate. Fe(III)-oxyhydroxide-coated sand was present as 10% of the mass, and it was arranged in three treatments: (1) homogeneously distributed, and present as a discrete layer (2) at the top and (3) at the bottom of 14-cm-long sand columns. A pulse input of 10⁸ cells ml^–1 was introduced in an artificial groundwater solution flowing at 14 cm h^–1 through the column, and eluted cells were counted. Peak breakthrough occurred at 1.0 pore volume. A large proportion of cells were retained; 14.7–15.8% of the cells were recovered after three pore volumes of solution had eluted through clean quartz sand, and only 2.1–4.0% were recovered from the Fe(III)-oxyhydroxide-coated sand mixtures. The three physical arrangements of the chemical heterogeneity resulted in essentially the same breakthrough of cells, indicating that the spatial distribution of iron coating does not affect the transport of bacteria. The results of the column transport experiments, which mimic hydrogeological conditions encountered in field problems, are consistent with our mechanistic understanding of bacterial sorption. Received: 10 April 1996 · Accepted: 17 February 1997     

Geographical distribution of general aerobic heterotrophic bacteria in surficial sediments from the Chukchi Sea and Canadian Basin

This paper determined the abundance of General Aerobic heterotrophic Bacteria(GAB) in surficial sediments from the Chukchi Sea and the Canadian basin by using MPN and discussed their geographical distribution.The result shows that the determination percentages of the GAB were high,even till 100 percentage.The abundance range and averages of GAB for 4℃and 25℃ were from 4.00×102 to 2.40×106,1.71×106 ind.·g-1(wet sample) and from 2.40×105 to 2.40×107,1.10×107 ind.·g-1(wet sample) respectively.Not only the abundance range but also the averages of GAB in 25℃ were higher than that in 4℃.The abundance of GAB in sediments show a tendency that it is roughly greater in the lower latitudinal area than in the higher latitudinal area.The abundance of GAB increased from east to west as for the longitudinal distribution.With the water depth increasing,the abundance of GAB at 4℃ decreased,but GBA at 25℃ is not changed obviously with water depth.It seems that warmer circumstantial temperature is more suitable for some GAB.