基于无人船的大洋中尺度涡观测系统展望

中尺度涡在大洋中普遍存在,研究发现其能量比大尺度海洋环流的能量大一个量级,在海洋物质能量输运和全球气候变化中起着重要的作用。受观测条件限制,目前对中尺度涡的观测主要通过卫星高度计实现,只能从海面高度来推算中尺度涡大小、分布、强度及其伴随的水体和能量输送,而卫星高度计对中尺度涡垂直结构特征认识不足,也导致了对中尺度涡所引起的上层海洋能量、热量输送估计误差偏大。目前对中尺度涡三维结构观测认识不足,展望未来将会出现基于无人船平台的大洋中尺度涡三维结构自动观测系统,该平台将集成自动水下剖面观测功能等先进技术,以便观测中尺度涡的垂直结构特征及其时空变化特征,进而可对中尺度涡带来的物质和能量输送进行系统认识。     

Seasonal variation and principle of cyanobacterial biomass and forms in the water source area of Chaohu City,China

We investigated seasonal variations in cyanobacterial biomass and the forms of its dominant population(M. aeruginosa) and their correlation with environmental factors in the water source area of Chaohu City,China from December 2011 to October 2012. The results show that species belonging to the phylum Cyanophyta occupied the maximum proportion of phytoplankton biomass,and that the dominant population in the water source area of Chaohu City was M. aeruginosa. The variation in cyanobacterial biomass from March to August 2012 was well fitted to the logistic growth model. The growth rate of cyanobacteria was the highest in June,and the biomass of cyanobacteria reached a maximum in August. From February to March 2012,the main form of M.aeruginosa was the single-cell form; M.aeruginosa colonies began to appear from April,and blooms appeared on the water surface in May. The maximum diameter of the colonies was recorded in July,and then gradually decreased from August. The diameter range of M. aeruginosa colonies was 18.37–237.77 μm,and most of the colonies were distributed in the range 20–200 μm,comprising 95.5% of the total number of samples. Temperature and photosynthetically active radiation may be the most important factors that influenced the annual variation in M. aeruginosa biomass and forms. The suitable temperature for cyanobacterial growth was in the range of 15–30°C. In natural water bodies,photosynthetically active radiation had a significant positive influence on the colonial diameter of M.aeruginosa(P0.01).     

Importance of kelp-derived organic carbon to the scallop <Emphasis Type="Italic">Chlamys farreri</Emphasis> in an integrated multi-trophic aquaculture system

Bivalves and seaweeds are important cleaners that are widely used in integrated multi-trophic aquaculture (IMTA) systems. A beneficial relationship between seaweed and bivalve in the seaweed-based IMTA system has been confirmed, but the trophic importance of seaweed-derived particulate organic materials to the co-cultured bivalve is still unclear. We evaluated the trophic importance of the kelp Saccharina japonica to the co-cultured scallop Chlamys farreri in a typical IMTA farm in Sungo Bay (Weihai, North China). The dynamics of detritus carbon in the water were monitored during the culturing period. The proportion of kelp-derived organic matter in the diet of the co-cultured scallop was assessed via the stable carbon isotope method. Results showed that the detritus carbon in the water ranged from 75.52 to 265.19 μg/L, which was 25.6% to 73.8% of total particulate organic carbon (TPOC) during the study period. The amount of detritus carbon and its proportion in the TPOC changed throughout the culture cycle of the kelp. Stable carbon isotope analysis showed that the cultured scallop obtained 14.1% to 42.8% of its tissue carbon from the kelp, and that the percentages were closely correlated with the proportion of detritus carbon in the water (F =0.993, P= 0.003). Evaluation showed that for 17 000 tons (wet weight) of annual scallop production, the kelp contributed about 139.3 tons of carbon (535.8 tons of dry mass). This confirms that cultured kelp plays a similar trophic role in IMTA systems as it does in a natural kelp bed. It is a major contributor to the detritus pool and supplies a vital food source to filter-feeding scallops in the IMTA system, especially during winter and early spring when phytoplankton are scarce.     

大黄鱼白细胞介素17受体E-like的分子特征及进化分析

白细胞介素17受体(Interleukin 17 receptor,IL-17R)家族与IL-17细胞因子家族介导的各类反应有着密切联系.IL-17RE-like(IL-17REL)是一类最新发现的IL-17受体,其结构与IL-17RE相似,但尚未发现其配体.本研究克隆得到大黄鱼(Larimichthys crocea)IL-17REL(Lyc IL-17REL)的全长c DNA,其开放阅读框全长1 659个核苷酸,推断可编码一个由552个氨基酸组成的蛋白,含有信号肽、胞外段、跨膜区和胞内段.与其他物种的IL-17REL相似,Lyc IL-17REL蛋白的胞外段具有一个保守的FN I-like结构域,而胞内段缺少IL-17受体家族保守的SEFIR(SEF and IL-17R)结构域.多序列比对分析表明Lyc IL-17REL与日本河豚(Takifugu rubripes)IL-17REL的氨基酸序列同源性最高,序列一致性可达57.0%.系统进化分析表明,脊椎动物的IL-17受体家族可分为2类,IL-17RA/RB/RD为一类,IL-17RC/RE/REL为一类.其中鱼类及高等脊椎动物的IL-17REL与IL-17RE亲缘关系最近,并且推测二者可能是由同一基因从硬骨鱼类之前的物种中分化而来.组织分布表明Lyc IL-17REL在所有被检测的组织中为组成型表达,在黏膜相关组织如小肠和鳃中的表达量最高.大黄鱼受嗜水气单胞菌(Aeromonas hydrophila)诱导刺激后,Lyc IL-17REL在其头肾及鳃中的基因转录水平均显著上调.这表明Lyc IL-17REL可能在细菌感染引起的免疫反应中起着重要作用.     

Soil phosphorus composition and phosphatase activities along altitudes of alpine tundra in Changbai Mountains,China

Alpine tundra ecosystems have specific vegetation and environmental conditions that may affect soil phosphorus(P) composition and phosphatase activities. However, these effects are poorly understood. This study used Na OH-EDTA extraction and solution31 P nuclear magnetic resonance(NMR) spectroscopy to determine soil P composition and phosphatase activities, including acid phosphomonoesterase(Ac P), phosphodiesterase(PD) and inorganic pyrophosphatase(IPP), in the alpine tundra of the Changbai Mountains at seven different altitudinal gradients(i.e., 2000 m, 2100 m, 2200 m, 2300 m, 2400 m, 2500 m, and 2600 m). The results show that total P(TP), organic P(OP), OP/TP, Na OH-EDTA extracted P and AcP, PD, and IPP activities over the altitude range of 2500–2600 m are significantly lower than those below 2400 m. The dominant extracted form of P is OP(73%–83%) with a large proportion of monoesters(65%–72%), whereas inorganic P is present in lower proportions(17%–27%). The activity of Ac P is significantly positively correlated with the contents of soil OP, total carbon(TC), total nitrogen(TN), and TP(P 0.05), indicating that the Ac P is a more sensitive index for responding P nutrient storage than PD and IPP. Soil properties, P composition, and phosphatase activities decrease with increased altitude and soil p H. Our results indicate that the distribution of soil P composition and phosphatase activities along altitude and Ac P may play an important role in P hydrolysis as well as have the potential to be an indicator of soil quality.     

Emissions of biogenic sulfur gases (H<Subscript>2</Subscript>S,COS) from <Emphasis Type="Italic">Phragmites australis</Emphasis> coastal marsh in the Yellow River estuary of China

Emissions of biogenic sulfur gases (hydrogen sulfide (H₂S) and carbonyl sulfide (COS)) from Phragmites australis coastal marsh in the Yellow River estuary of China were determined during April to December in 2014 using static chamber-gas chromatography technique with monthly sampling. The results showed that the fluxes of H₂S and COS both had distinct seasonal and diurnal variations. The H₂S fluxes ranged from 0.09 μg/(m²·h) to 7.65 μg/(m²·h), and the COS fluxes ranged from–1.10 μg/(m²·h) to 3.32 μg/(m²·h). The mean fluxes of H₂S and COS from the P. australis coastal marsh were 2.28 μg/(m²·h), and 1.05 μg/(m²·h), respectively. The P. australis coastal marsh was the emission source of both H₂S and COS over the whole year. Fluxes of H₂S and COS were both higher in plant growing season than in the non-growing season. Temperature had a dramatic effect on the H₂S emission flux, while the correlations between COS flux and the environmental factors were not found during sampling periods. More in-depth and comprehensive research on other related factors, such as vegetation, sediment substrates, and tidal action is needed to discover and further understand the key factors and the release mechanism of sulfur gases.     

Influencing factors of seed long-distance dispersal on a fragmented forest landscape on Changbai Mountains,China

Seed long-distance dispersal(LDD) events are typically rare, but are important in the population processes that determine large-scale forest changes and the persistence of species in fragmented landscapes. However, previous studies focused on species dispersed via animal-mediated LDD, and ignored those dispersed by wind. The aim of this study was to assess the effects of canopy openness, edge, seed source, and patch tree density on the LDD of seeds by wind in forest. We collected birch seeds, a typical wind-dispersed species, throughout a larch plantation. We then assessed the relationship between birch LDD and each factor that may influence LDD of seeds by wind including distance to edge, canopy openness size, distance to mature forest, and the tree density of the larch plantation. We used univariate linear regression analysis to assess the influence of those factors on birch LDD, and partial correlations to calculate the contribution of each factor to LDD. The results showed that both canopy openness and edge had significant influences on birch LDD. Specifically, a negative relationship was observed between distance to edge and birch LDD, whereas there was a positive correlation between canopy openness size and LDD. In contrast, the distance to the mature forest was not correlated with birch LDD. Our results suggest that patch tree density could potently affect the probability of LDD by wind vectors, which provides novel and revealing insights regarding the effect of fragmentation on wind dynamics. The data also provide compelling evidence for the previously undocumented effect of habitat fragmentation on wind-dispersed organisms. As such, these observations will facilitate reasonable conservation planning, which requires a detailed understanding of the mechanisms by which patch properties hamper the delivery of seeds of wind-dispersed plants to fragmented areas.     

Difference in the influence of Indo-Pacific Ocean heat content on South Asian Summer Monsoon intensity before and after 1976/1977

Monthly ocean temperature from ORAS4 datasets and atmospheric data from NCEP/NCAR Reanalysis I/II were used to analyze the relationship between the intensity of the South Asian summer monsoon(SASM) and upper ocean heat content(HC) in the tropical Indo-Pacific Ocean.The monsoon was differentiated into a Southwest Asian Summer Monsoon(SWASM)(2.5°–20°N,35°–70°E) and Southeast Asian Summer Monsoon(SEASM)(2.5°–20°N,70°–110°E).Results show that before the 1976/77 climate shift,the SWASM was strongly related to HC in the southern Indian Ocean and tropical Pacific Ocean.The southern Indian Ocean affected SWASM by altering the pressure gradient between southern Africa and the northern Indian Ocean and by enhancing the Somali cross-equatorial flow.The tropical Pacific impacted the SWASM through the remote forcing of ENSO.After the 1976/77 shift,there was a close relationship between equatorial central Pacific HC and the SEASM.However,before that shift,their relationship was weak.     

Analysis of phenotypic and genetic parameters for growthrelated traits in the half smooth tongue sole, <Emphasis Type="Italic">Cynoglossus semilaevis</Emphasis>

Phenotypic and genetic parameters for growth-related traits in the half-smooth tongue sole, Cynoglossus semilaevis, were estimated in 22 full-sib families produced by normal and neo-male breeding stocks. As phenotypic males with female genotypes, neo-males are harmful in C. semilaevis aquaculture because they reduce overall production. The present study evaluated the difference in the growth-related traits: total length (TL), body weight (BW) and square root of body weight (SQ_BW) at the age of 570 days between normal and neo-male offspring (neo-males used as male parents). The difference in the proportion of females between normal and neo-male offspring was also assessed. Based on the linear mixed model, restricted maximum likelihood (REML) and best linear unbiased prediction (BLUP) were used to estimate various (co)variance components and estimated breeding values (EBVs) of growth-related traits. As a result, all the mean values of the three studied traits were significantly larger in normal offspring than in neo-male offspring. Additionally, the female proportion was significantly larger in normal offspring than in neo-male offspring. Heritability was 0.128±0.066 2 for TL, 0.128±0.065 5 for BW and 0.132±0.062 9 for SQ_BW, all of which were low level heritabilities. The correlation coefficients of EBVs and phenotypic values of the target traits were 0.516 for TL, 0.524 for BW and 0.506 for SQ_BW, all of which were highly significant (P <0.01). Genetic correlations among TL, BW and SQ_BW were positive high (0.921–0.969) and higher than those of phenotype (0.711–0.748), both of which had low standard errors (0.063–0.123 for genotype, and 0.010–0.018 for phenotype). Compared with normal offspring, neo-male offspring have lower breeding values for each studied trait through EBVs comparison. Therefore, neo-male offspring should not be used as broodstock in a C. semilaevis breeding programs.     

Involvement of cyclic electron flow in irradiance stress responding and its potential regulation of the mechanisms in <Emphasis Type="Italic">Pyropia yezoensis</Emphasis>

Pyropia yezoensis, belongs to the genus of Porphyra before 2011, inhabit on intertidal zone rocks where irradiation changes dramatically, implying that the seaweed has gained certain mechanisms to survive a harsh environment. Based on the photosynthetic parameters with or without the inhibitors determined by a Dual-PAM-100 apparatus, we investigated the photosynthetic performance and the changes in electron flow that occurred during the algae were stressed with different light intensities previously. When the irradiation saturation was approaching, the CEF around PS I became crucial since the addition of inhibitors usually led to an increase in non-photochemical quenching. The inhibitor experiments showed that there were at least three different CEF pathways in Py. yezoensis and these pathways compensated each other. In addition to maintaining a proper ratio of ATP/NAD(P)H to support efficient photosynthesis, the potential roles of CEF might also include the regulation of different photoprotective mechanisms in Py. yezoensis. Under the regulation of CEF, chlororespiration is thought to transport electrons from the reduced plastoquinone (PQ) pool to oxygen in order to mitigate the reduction in the electron transfer chain. When irradiation was up to the high-grade stress conditions, the relative value of CEF began to decrease, which implied that the NADP⁺ pool or PQ⁺ pool was very small and that the electrons were transferred from reduced PS I to oxygen. The scavenging enzymes might be activated and the water-water cycle probably became an effective means of removing the active oxygen produced by the irradiation stressed Py. yezoensis. We believe that the different mechanisms could make up the photoprotective network to allow Py. yezoensis for survival in a highly variable light stress habitat, which may enlighten scientists in future studies on irradiance stress in other algae species.