Experimental study of the fine structure of the wind field near a rough surface

The air-flow velocity field near the water surface is studied in the zone of wind-wave intensification. Caused by a periodic separation of eddies, a nonzero time-averaged value of the wind velocity in the near-water streamline is detected at the leading slope of the wave. The distribution of pressure along the wave with allowance for the vertical velocity shear and disturbances produced by eddies and a periodic deceleration of the viscous layer was calculated with the aid of the Cauchy-Lagrange integral. This procedure made it possible to calculate the growth rate of the wave amplitude, whose value was found to be close to its experimental value at the initial stage of acceleration.     

钝顶螺旋藻藻蓝蛋白和多糖的抗肿瘤免疫活性研究

对钝顶螺旋藻藻蓝蛋白 (PC)和多糖 (PSP)的抗肿瘤免疫活性进行了研究。采用免疫酶标技术、MTT法及定量溶血分光光度测定法等免疫分析实验 ,从免疫器官、免疫细胞、免疫分子 3个水平上进行藻蓝蛋白和多糖的抗肿瘤免疫活性检测。结果显示 ,藻蓝蛋白和多糖处理组小鼠瘤块直径及瘤体重量均小于对照组 ,T细胞及 B细胞活性明显增强 ,体液抗体量显著提高 ,螺旋藻藻蓝蛋白比多糖抑制肿瘤细胞生长和提高机体免疫力的作用更明显。     

海水中低含量铵氮的高灵敏度荧光法测定

提出了一种改进的、可测定海水中低含量铵氮的高灵敏度荧光分析方法。其原理是在硼酸缓冲溶液的作用下,海水中的铵氮与邻苯二甲醛(OPA)发生衍生化反应,通过测定生成的荧光产物来确定水体中铵氮的浓度。方法的检测限极低(0.002 5μmol/L),重现性好,且水样用量少(10 mL),适用于海水中溶解有机物的光铵化研究及其他低铵含量水样的测定。     

青岛近海地区SO2和SO42-干沉降速率的季节变化

用小麦岛和团岛观测站提供的青岛近海气象和水温资料,以适于SO2气体和硫酸盐(SO42-)气溶胶的干沉降模型研究了模型中的输送阻尼对两种污染物质干沉降速率的贡献,并计算了2003年青岛近海地区两种污染物质干沉降速率的季节变化。结果表明:对SO2而言,粘性副层传输系数对沉降速率的贡献大于空气动力学传输系数;对于SO42-,空气动力学传输系数对沉降速率的贡献要大于表面传输系数。SO2和SO42-的干沉降速率变化范围分别为0.187~0.868cm/s和0.188~0.532cm/s。两种污染物质干沉降速率的四季变化有相似的规律,即冬季>秋季>春季>夏季。     

Thermohydrodynamics of the ocean decay of nonlinear oscillations in a bounded basin of variable depth

We study the time decay of surges of a liquid in a round shallow-water basin of variable depth. The dependence of the logarithmic decrement of oscillations on the bottom topography and wind velocity is analyzed. The role of convective acceleration and bottom friction in the formation of both the level of vertical displacement of the surface of the basin and the velocity field of horizontal wave currents is estimated. __________ Translated from Morskoi Gidrofizicheskii Zhurnal, No. 2, pp. 3–11, March–April, 2006.     

渤海裸甲藻和链状亚历山大藻的麻痹性贝毒毒素分析

目的:分析渤海裸甲藻(Gymnodinium sp.)和链状亚历山大藻(Alexandrium catenella)的麻痹性贝毒毒素,为渤海天津海域的赤潮研究积累基础数据。方法:通过实验室培养裸甲藻和链状亚历山大藻,选取对数生长期、平台生长期的裸甲藻以及平台生长期的链状亚历山大藻,利用高效液相色谱法(HPLC)对这两种微藻进行麻痹性贝毒(PSP)毒素分析。结果:裸甲藻细胞内不含有麻痹性贝毒(PSP);链状亚历山大藻细胞内含有C毒素和GTX1-4毒素,该微藻每个细胞毒素含量约为10.81 fmol/cell。结论:裸甲藻细胞内虽不含有麻痹性贝毒(PSP),但不能排除其含有其它毒素的可能。链状亚历山大藻细胞内含有麻痹性贝毒(PSP),属于有毒微藻,需要对其进行密切监测。     

栉江珧工厂化育苗技术研究

通过亲贝强化营养和升温促熟培育,提早栉江珧人工育苗时间;合理控制幼虫密度,前期4~5个/mL,后期2~3个/mL,严把饵料质量关,投喂新鲜无污染的饵料,科学换水与充气,及时分级筛选等系列技术措施,克服栉江珧育苗中易发生幼虫粘连的技术难题,使育苗生产顺利进行;开展了多种附着基投放的采苗方法试验,选出了铺砂浮动网箱采苗和网袋装网片附着基和细砂吊在池中采苗2种比较理想的采苗方法,提高稚贝的附着变态率,其附着变态率达30%以上,促进稚贝的生长和成活,在1 000 m3水体中育出2 mm以上的稚贝1.096亿粒。     

黄茅海河口湾现代动力地貌体系和冲淤过程分析

1980—1993年对黄茅海河口湾进行沉积物采样和水流测定及水深测量。根据水动力和地形条件，冲淤分析及Mclaren模型研究河口湾的动力地貌体系、冲淤特征和现代沉积物运移。结果表明：（1）水下地形主要为下泄流或上溯流控制的“深槽－槽沟－浅滩－湾口”的动力地貌体系，反映了河口湾“东进西出”的水流格局；（2）整个河口湾以淤积为主，只有崖门深槽有较明显的优势冲刷特征，并随着崖门深槽向海推移和河口湾“东进西出”水动力作用，黄茅海落潮三角洲相应向西南进积；（3）应用Mclaren模型揭示了黄茅海河口湾现代沉积物运移规律，同样反映了河口湾具有“东进西出”的运移趋势。     

Application of the first internal transcribed spacer(ITS-1) of ribosomal DNA as a molecular marker to population analysis in farrer''s scallop Chlamys farreri

Sequence variation of the first internal transcribed spacer of ribosomal DNA （ ITS - 1 ） was examined and its application to the study of genetic variation was explored in four populations of farter＇ s scallop Chlamys farreri. ITS - 1 fragments, with a length of about 300 bp,of 78 individuals collected from Dalian, Qingdao, Yantai in China and Korea respectively were amplified via PCR, cloned and sequenced. Intra-genomic variation was examined by sequencing several clones of single individuals. Alignment and polymorphism analysis detected 44 haplotypes and 50 polymorphic sites which consist of 30 substitutions and 20 indels, indicating a high level of polymorphisms. Sequence analysis also showed a very low level of intra-individual variation. All these features validated the feasibility of application of ITS - 1 fragment to population analysis. Polymorphism analysis showed that the Korea sample has the richest genetic variation, followed by Yantai and Qingdao samples. AMOVA （analysis of molecular variance） showed that the majority （96.26%） of genetic variation was distributed within populations and 3.74% resulted from among populations, but with P 〈 0.05 （ = 0.042）, indicating that the populations in this study have significant divergence. This output was basically concordant with the result arising from RAPD data and different from that from mitochondrial 16S rDNA sequence data. Discussion on this inconsistency was made accordingly.     

基于声学调制解调器的声速剖面遥测技术

声速是海洋声呐测量中最重要的声学参数之一。拖缆式声速剖面仪可提供实时测量功能,但其缆长不能满足深海测量要求;自容式声速剖面仪可满足深海测量要求,但工作效率低。如何提高野外深海声速剖面测量工作效率是深海调查一项非常有意义的工作。文中详细介绍了基于声学调制解调器的声速剖面遥测系统的主要结构和功能,揭示了水声通信技术在深海非铠装缆测量中的应用优势。