96孔酶标板法测定中国对虾血淋巴上清液抗菌活力和酚氧化酶活性的初步研究

用96孔酶标板法测量单尾中国对虾血淋巴上清液抗菌（Uα）应力和过氧化酶活性。实验表明，该法具有以下优点：1）可测定大批单尾对虾血淋巴上清液的抗菌活力，酚氧化酶活性；2）所需对虾血淋巴上清液微量化；3）用酶标仪读取酶的活性，减少了不同试验者人为读数时的误差；4）重复性高。     

GPS在海洋执法管理方面的应用--HYQ2-1型非自航式倾废航行数据记录仪

本文介绍了海洋技术研究所研制非自航式倾废航行数据记录仪的背景、目的和意义，概述了仪器的组成、原理及工作流程，阐述了地面处理系统的功能，并对仪器的发展前景进行了探讨。     

偏振激光散斑的相关性研究

采用发送一束线偏振激光产生散斑的方法，研究线偏振激光散斑、＂平均椭圆偏振＂激光散斑以及非偏振激光散斑的相关性和精细结构。指出：当漫射器为一片毛玻璃时，散斑激光是线偏振的．其方向与入射激光束相同；当漫射器为涂无光白漆的毛玻璃时，散斑激光是部分或全部退偏的，涂层越厚，退偏越甚。对于强线偏振散斑场，当偏振方向改变时，散斑的精细结构无明显变化，即使是两个正交方向上的散斑图，也保持良好的相关性。对于非偏振散斑场，当照明激光束的偏振方向改变时．散斑精细结构有明显变化。     

南海深海盆地沉积柱样中的浊流沉积

本文根据南海深海盆地三个沉积柱样的粒度结构、地球化学、微体古生物等特征分析,深讨了南海深海盆地细粒沉积物的浊积现象。结果表明,位于南海北部陆架斜坡上KL37孔的浊流沉积现象并不明显;位于陆架斜坡和深海盆地交界处的KL29孔存在着大量的浊积层,属于浊流沉积和半远洋沉积环境;位于南海盆地中部的KL91孔虽然已属于远洋性沉积环境,但除出现火山灰沉积外,浊流沉积作用仍然是相当活跃的。     

ISOLATION OF DNA FROM SEA URCHIN SPERM NUCLEI BY HYDROXYAPATITE CHROMATOGRAPHY

This article describes in detail the procedures in obtaining spermatids from living animals and in isolating sperm nuclei DNA by hydroxyapatite chromatography. The isolated DNA is free of RNAs and proteins after treatment with proteinase K, followed by chromatography on hydroxyapatite column which is eluted with stepwise-increased concentrations of potassium phosphate buffer. More than 89% of the DNA purified is found in the eluates of both 0.2 M and 0.25 M potassium phosphate buffer. The two fractions are identical in ultraviolet absorption spectrum. DNA molecules thus prepared should be useful for studying DNA structure and DNA replication by electron microscopy, since the length of the purified DNA molecules is in the range of the size of a replicating unit.     

中国南极中山锚地勘测

本文介绍了南极中山锚地的选择条件,勘测实施过程及水文、气象特点,并对测量区域作了分析研究与评价,科学地确定了适合科考船抛锚的锚地,致使“雪龙”船首次在中山锚地抛锚试抛成功,结束了中山站附近海域无锚地的历史。     

不同软基处理方法适用性比较

总结分析了国内外现有的软土地基处理的各种方法。通过对采用不同方法进行软基处理的工程实例处理效果的定量对比，发现不同软基处理方法的适用性差异很大，多数工程中仅用一种方法很难达到设计要求，而两种或多种方法联合则可望取得更好的效果。     

动荷载作用下海底粉土的孔压响应及其动强度

本文选用近海分布较广的粉土为研究对象 ,利用室内动三轴试验结果 ,找出动荷载作用下粉土的动应力应变关系 ,分析模拟波浪荷载作用下粉土中的孔压响应、临界循环次数 ,确定波浪作用下粉土的应力状态、破坏临界循环次数 ,判断不同深度处的粉土发生液化的可能性及发生液化所需要的时间 ;研究粉土在动荷载作用下的强度降低 ,为海上工程设计和施工提供科学依据。     

Effects of chemical ecological adjustment and control experiment on phytoplankton community in the Aoshan Bay

There is a low nutrient level in the Aoshan Bay. In June 1999, the chemical adjustmentand control experiment was made in the Aoshan Bay. Following tracts investigation was carried out before the experiment and on the 1st, 2nd, 4th, 5th, 6th and 45th day/after the experiment. While the variance of amount of phytoplankton, the replacement of superior species and the species composition of phytoplankton were researched. The results show that the amount of phytoplankton in the Aoshan Bay rises gradually after the experiment. Ceratium macroceros Cleve of pyrophyta was the dominant species before the experiment, its dominant index was 37.7％. Six days after the experiment, its dominant index dropped to 17.6％ . Meanwhile the dominant index of Asterionella japanics Cleve rose from 7.1％ to 39.2％ , it became the first dominant species. Forty-five days after the experiment, the amount of phytoplankton in the Aoshan Bay was 5.15 to 137.32 times more than that in 1997.     

魁蚶线粒体16S rRNA和COI基因片段序列测定及其应用前景

魁蚶（Ｓｃａｐｈａｒｃａｂｒｏｕｇｈｔｏｎｉｉ）是蚶科贝类的一种大型经济种类，主要分布于我国、日本和朝鲜半岛及俄罗斯东南部沿海。在我国，主要分布于辽宁、河北和山东沿海，生活在３～５０ｍ（多为２０～３０ｍ）水深的软泥或泥沙质海底，是我国北方沿海重要的经济贝类之一。但但有有关关其其自自然然群群体体的的遗遗传传变变异异及及群群体体间间遗遗传传分分化化等等方方面面的的研研究究不不多多 ;;同同时时，，作作者者和和日日本本研研究究者者发发现现，，中中国国、、韩韩国国和和日日本本魁魁蚶蚶群群体体在在形形态态和…