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71.
72.
固定化鲅鱼乙酰胆碱酯酶的制备及部分性质测定 总被引:3,自引:0,他引:3
酶的固定化是酶传感器制备过程中重要的1个环节.研究采用直接共价法固定鲅鱼乙酰胆碱酯酶(AChE).制备方法为:0.1g CNBr活化的Sepharose 4B凝胶用1mmol/L的HCl充分溶胀后,与活力为10U的AChE溶液混合,于4℃下150r/min振荡8h.所制备的固定化酶活力回收率较高(96%),对pH值和温度变化的适应能力均优于非固定化酶;在3个月保存期内,前者的活力损失13%,而后者的活力则下降89%.这说明固定过程能够大大提高AChE的抗逆性和保持酶活力的稳定,有利于酶传感器的制备. 相似文献
73.
Qiu Dahong Zang Jun Jia Ying
Academician of the Chinese Academy of Sciences Professor the State Key Laboratory of Coastal Offshore Engineering 《中国海洋工程》1996,(2)
Based on the 2nd order cnoidal wave theory, the characters of shallow water standing waves and their action on vertical walls are studied in this paper. The theoretical expressions of the wave surface elevation in front of and the wave pressure on the vertical wall are obtained. In order to verify the theoretical results, model tests were made in the State Key Laboratory of Coastal and Offshore Engineering at DUT. For the wave surface elevation in front of the wall and the wave forces on the wall at the moment when the wave surface at the wall surface goes down to the bottom of the wave trough, the calculated results coincide quite well with the experimental results. For the wave forces on the wall at the moment when the wave surface at the wall surface goes up to the top of the wave crest, the theoretical expressions are modified by the experimental results. For the convenience of practical use, calculations are made for the wave conditions which usually occur in enginering practice by use of the inves 相似文献
74.
You Yuming Hou Min Senior Engineer River Harbour Department Nanjing Hydraulic Research Institute Nanjing Assistant Engineer River Harbour Department Nanjing Hydraulic Research Institute Nanjing 《中国海洋工程》1992,(3)
- The data of landsat TM of multi-temporal for Lingdingyang Estuary, Pearl River in China is firstly used with suspended sediment concentration of field measurement to establish a correlative model equation. After the ratio processing of TM data and atmospheric correction, the images of suspended sediment concentration of different temporals are exported from the image processing systems AREIS II and III. These images express the characteristics of suspended sediment distribution, the mode of sediment transport and the extent of dispersion under the actions of tidal current and wind condition of different seasons. 相似文献
75.
Cao Guoao Shen Yilei
Dr.Professor Tongji University Shanghai
Shanghai College of Architectural Municipal Engineering Shanghai 《中国海洋工程》1991,(1)
In this paper, the responses of the interaction system of R.C. gravity single-leg platform to seismic excitation are mainly analysed. A set of nonlinear equations for the interaction system are established by using the wave, one is the soil-structure interaction and the other is the fluid-structure interaction. The seismic response of the interaction system is analysed for the influence of the asymmetric structure, fluid action, etc. with the input of seismic SH waves in any direction. The numerical results are given for a simple example. 相似文献
76.
A new approach to high-order Boussinesq-type equations with ambient currents is presented. The current velocity is assumed to be uniform over depth and of the same magnitude as the shallow water wave celerity. The wave velocity field is expressed in terms of the horizontal and vertical wave velocity components at an arbitrary water depth level. Linear operators are introduced to improve the accuracy of the kinematic condition at the sea bottom. The dynamic and kinematic conditions at the free surface are expressed in terms of wave velocity variables defined directly on the free surface. The new equations provide high accuracy of linear properties as well as nonlinear properties from shallow to deep water, and extend the applicable range of relative water depth in the case of opposing currents. 相似文献
77.
温度、pH对缢蛏(Sinonovacula constricta)消化酶活力的影响 总被引:8,自引:0,他引:8
本文研究了温度、pH对缢蛏(Sinonovacula constricta(Lamarck))三种主要消化酶(蛋白酶、淀粉酶和纤维素酶)活力的影响。结果表明:当温度在5~75℃之间时,蛋白酶、纤维素酶和淀粉酶的最适温度分别为为55℃、45℃和65℃;在不同的pH范围内,蛋白酶的最适PH值为9.2,淀粉酶的最适PH值为6.3和7.7,纤维素酶的最适PH值为5.2。缢蛏的淀粉酶和蛋白酶活力较大,纤维素酶活力极微。 相似文献
78.
79.
1 .IntroductionTheBohaiGulfisinfestedbyiceinthewholewinter,andoffshorestructuresthereexperiencenotonlywind ,waves ,currentsandearthquake ,butalsoiceloadswhicharecomplicatedbecauseofthedynamicinteractionbetweenicesheetsandstructures.Duanetal.( 2 0 0 0 )hadacomprehensivere viewofthelatestadvancesonice inducedvibration ,fatigueandfractureofoffshorestructures ,payingmuchattentiontothedifferencesinicebreakingmodeanddynamicsofstructuresindifferentice in festedwaters .Theypresentedcriticallythelates… 相似文献
80.
Li Yucheng Wang Fenglong Professor Dalian University of Technology Dalian Senior Engineer Dalian University of Technology Dalian 《中国海洋工程》1993,(1)
- The wave-current forces on vertical piles in side-by-side arrangement induced by irregular waves with opposing current are investigated experimentally in this paper. The characteristics in both time and frequency domain of in-line, lift and resultant forces are analyzed. The grouping effect coefficients of inline, lift and resultant forces on piles related to KC number and relative spacing parameters are given. These results are compared with those in the case of irregular waves combined with following currents. It is found that the results in these two cases are quite different. The range of KC number tested is 10- 60, the range of Reynolds number is (0.55-3.43) ×104. 相似文献