冬季和春季长江口及其近海水域浮游病毒丰度的分析

采用荧光显微技术,对2006年长江口及近海水域20个站点的表层及10m层或潜水体冬、春两季的浮游病毒丰度进行了检测,对浮游病毒丰度在季节（冬、春两季）、水平分布和垂直分布上的变化进行了探讨.调查区浮游病毒丰度在冬、春季节上并无明显差异,但在水平分布上存在很大差异,河口区浮游病毒直接检测量（Virus Direct Count, VDC）达到10^7个/ml,近海水域VDC为10^6个/ml,河口区的浮游病毒丰度都明显高于近海水域病毒丰度 （P＜0.01）.在垂直分布上,冬、春两季长江口水域水深小于10m的站位,表层浮游病毒丰度与底层病毒丰度无明显差别,水深大于10m的站位,表层水样的浮游病毒丰度都高于10m水层病毒丰度,说明长江口浮游病毒的垂直分布与站位总水深有关.还通过比较各站点VDC与叶绿素a含量的数据,分析了二者之间的相关性：冬季浮游病毒丰度与叶绿素a含量成正相关性;春季浮游病毒丰度与叶绿素a含量成负相关性,但病毒丰度受叶绿素a含量的影响仅为10%-11%.     

毛蚶人工育苗技术

根据对毛蚶性腺发育、产卵孵化、浮游幼虫及稚贝习性等的研究，初步探索了其人工育苗中主要技术环节。2龄毛蚶全为雄性或雌雄同体，3龄以上群体雌性占70％以上。26．6～27．4℃，受精卵22h发育到D形幼虫；26．5～23．0℃，D形幼虫经过15天发育到眼点幼虫；25．O～20．0℃，投附着基、13天左右稚贝壳长达到600μm时，可以出池到室外进行中间育成。     

固定化鲅鱼乙酰胆碱酯酶的制备及部分性质测定

酶的固定化是酶传感器制备过程中重要的1个环节.研究采用直接共价法固定鲅鱼乙酰胆碱酯酶（AChE）.制备方法为：0.1g CNBr活化的Sepharose 4B凝胶用1mmol/L的HCl充分溶胀后,与活力为10U的AChE溶液混合,于4℃下150r/min振荡8h.所制备的固定化酶活力回收率较高（96%）,对pH值和温度变化的适应能力均优于非固定化酶;在3个月保存期内,前者的活力损失13%,而后者的活力则下降89%.这说明固定过程能够大大提高AChE的抗逆性和保持酶活力的稳定,有利于酶传感器的制备.     

Stability Design System of Submarine Pipelines

This paper introduces a stability design system of submarine pipelines. The system can provide a reasonable design procedure for users with reference to domestic and foreign methods and in the light of home engineering experience.     

A New Approach to High-Order Boussinesq-Type Equations with Ambient Currents

A new approach to high-order Boussinesq-type equations with ambient currents is presented. The current velocity is assumed to be uniform over depth and of the same magnitude as the shallow water wave celerity. The wave velocity field is expressed in terms of the horizontal and vertical wave velocity components at an arbitrary water depth level. Linear operators are introduced to improve the accuracy of the kinematic condition at the sea bottom. The dynamic and kinematic conditions at the free surface are expressed in terms of wave velocity variables defined directly on the free surface. The new equations provide high accuracy of linear properties as well as nonlinear properties from shallow to deep water, and extend the applicable range of relative water depth in the case of opposing currents.     

温度、pH对缢蛏(Sinonovacula constricta)消化酶活力的影响

本文研究了温度、pH对缢蛏(Sinonovacula constricta(Lamarck))三种主要消化酶(蛋白酶、淀粉酶和纤维素酶)活力的影响。结果表明：当温度在5～75℃之间时，蛋白酶、纤维素酶和淀粉酶的最适温度分别为为55℃、45℃和65℃；在不同的pH范围内，蛋白酶的最适PH值为9．2，淀粉酶的最适PH值为6．3和7．7，纤维素酶的最适PH值为5．2。缢蛏的淀粉酶和蛋白酶活力较大，纤维素酶活力极微。     

优选沙子口污水处理厂排海口位置的研究

对青岛沙子口污水处理厂排海口位置进行了水动力条件、混合区域海水交换率及更新周期、污染物扩散系数及浓度分布、水质点迁移轨迹的比较，确定了其最佳排海口位置方案。     

Fuzzy Fatigue Reliability Analysis of Offshore Platforms in Ice-Infested Waters

1 .IntroductionTheBohaiGulfisinfestedbyiceinthewholewinter,andoffshorestructuresthereexperiencenotonlywind ,waves ,currentsandearthquake ,butalsoiceloadswhicharecomplicatedbecauseofthedynamicinteractionbetweenicesheetsandstructures.Duanetal.( 2 0 0 0 )hadacomprehensivere viewofthelatestadvancesonice inducedvibration ,fatigueandfractureofoffshorestructures ,payingmuchattentiontothedifferencesinicebreakingmodeanddynamicsofstructuresindifferentice in festedwaters .Theypresentedcriticallythelates…     

A Computational Model for Velocity Separation in Shallow Sea

Based on the hydrodynamical feature and the theoretical velocity profiles of tidal flow and vvind-induced flow in shal-low sea, a computational model is established for the first time, which can separate observed velocity into tidal velocity and wind-induced velocity by use of the least square method. With the model, not only the surface velocities of tidal flow and vvind-induced flow are obtained, but also the bed roughness height is determined and the wind velocity above the wa-ter surface is estimated. For verification of the model, the observed velocity in the Yellow River Estuary and the laborato-ry test is separated, then it is applied to the Yangtze River Estuary. All the results are satisfactory. The research results show that the model is simple in method, feasible in process and reasonable in result. The model is a valid approach to analysis and computation of field dala, and can be applied to separate the observed velocity in shallow sea; at the same time, reasonable boundary conditions of th     

浒苔(Enteromorpha prolifera)生活史的初步研究

通过实验室培养实验,全面地观察描述了浒苔生活史各个阶段的发育情况。雌雄异体的配子体成熟后,放散双鞭毛的配子,雌雄配子结合成合子进而发育成孢子体,配子也可以进行单性生殖发育成膜状的配子体。孢子体成熟放散四鞭毛的游孢子,游孢子固着后,可直接发育成配子体。其生活史是单倍体的配子体与二倍体的孢子体相互交替的同形世代交替。雌雄配子具有正趋光性,易大量聚集,结合后同游孢子一样都呈负趋光性。