牙鲆(Paralichthys olivaceus)β诺达病毒衣壳蛋白重组表达及复性条件优化 组表达及复性条件优化

β诺达病毒利用其缺乏校正功能的聚合酶进行基因组复制, 易导致突变, 因而具有广泛的宿主感染性, 而且可引发致死率极高的病毒性神经坏死症, 需要有针对性地研究检测及防御方法。本研究以感染牙鲆(Paralichthys olivaceus)的β诺达病毒为对象, 利用引物设计, 将His标签编码序列连接到完整病毒衣壳蛋白C端, 构建原核表达载体; 采用SDS-PAGE及质谱对表达产物进行分离和鉴定; 重组衣壳蛋白经镍离子亲和柱纯化后进行复性条件的正交优化。结果表明4个肽段经质谱鉴 定与预期一致; 纯化产物产量可达36mg/L; 4°C条件下, 复性缓冲液中尿素和PBS的最适浓度为0.8mol/L和0.05mol/L。本研究建立的制备方案可为研制相关疫苗以及开发针对该病毒的快速检测产品等提供有价值的参考。     

Distributional characteristics of grain sizes of surface sediments in the Zhujiang River Estuary

Distributions of the parameters of sedimentary grain sizes and their correlations were studied to trace the sources of silts and their movement trends in the Zhujiang River Estuary based on the analyses of grain sizes from more than 1080 sedimentary samples. The distributions of the median diameter, public value, quartile deviation, and skewness of sediments were complex in the Zhuiiang River Estuary mainly because of the impact of the matter source regions, distances from the source regions, and hydrodynamic conditions, such as waves, tidal currents, and coastal currents. Analyses of the parameters of the grain sizes for the various types of sediments showed that the distributions of the surface sediments in the Zhujiang River Estuary were controlled by many factors. Their matter sources were mainly the sediments discharged from the runoffs and ebb tidal currents, and from the open sea. The sediments mainly moved by suspension movement. The silts formed a large area of sediments with suspended fine silts in the Zhujiang River Estuary by internal adjustment transportation in the area, and moved toward the western coast of the Zhujiang River Estuary under the effect of Coriolis forces and coastal currents.     

南大西洋沉积物中多环芳香化合物的组成特征研究

10 samples of sediments obtained from the South Mid-Atlantic Ridge were measured for the abundances and distributions of polycyclic aromatic compounds(PAHs). The total concentrations of PAHs(∑PAHs) ranged from 2.768 to 9.826 μg/g dry sediment. The ∑PAHs was higher in sample 22V-TVG10 and sample 26V-TVG05 which were close to hydrothermal fields, with the lowest value in sample 22V-TVG14 which was farthest from hydrothermal fields, suggesting a probable hydrothermal origin of ∑PAHs of samples. Approximately nine kinds of PAHs were identified, and low molecular mass tricyclic and tetracyclic aromatic compounds were predominant in the samples. The concentrations of fluoranthene which were typical as hydrothermal alteration compounds were the highest among PAHs with dry weight between 0.913–3.157 μg/g. The phenanthrene homologue was most abundant in the samples, and the ratios between parent phenanthrene and methylphenanthrene which probably reflected the degree of hydrothermal alteration ranged from 0.097 to 1.602. The sample 22V-TVG10 possessing a maximum ratio value showed the intense influence of the hydrothermal alteration on this sample, which might further imply that PAHs in sediments were mainly derived from the hydrothermal alteration.     

琼东南盆地深水区构造热演化特征及其影响因素分析

To reveal the tectonic thermal evolution and influence factors on the present heat flow distribution, based on 154 heat flow data, the present heat flow distribution features of the main tectonic units are first analyzed in detail, then the tectonic thermal evolution histories of 20 profiles are reestablished crossing the main deep-water sags with a structural, thermal and sedimentary coupled numerical model. On the basis of the present geothermal features, the Qiongdongnan Basin could be divided into three regions: the northern shelf and upper slope region with a heat flow of 50–70 m W/m2, most of the central depression zone of 70–85 m W/m2, and a NE trending high heat flow zone of 85–105 m W/m2 lying in the eastern basin. Numerical modeling shows that during the syn-rift phase, the heat flow increases generally with time, and is higher in basement high area than in its adjacent sags. At the end of the syn-rift phase, the heat flow in the deepwater sags was in a range of 60–85 m W/m2, while in the basement high area, it was in a range of 75–100 m W/m2. During the post-rift phase, the heat flow decreased gradually, and tended to be more uniform in the basement highs and sags. However, an extensive magmatism, which equivalently happened at around 5 Ma, has greatly increased the heat flow values, and the relict heat still contributes about 10–25 m W/m2 to the present surface heat flow in the central depression zone and the southern uplift zone. Further analyses suggested that the present high heat flow in the deep-water Qiongdongnan Basin is a combined result of the thermal anomaly in the upper mantle, highly thinning of the lithosphere, and the recent extensive magmatism. Other secondary factors might have affected the heat flow distribution features in some local regions. These factors include basement and seafloor topography, sediment heat generation, thermal blanketing, local magmatic injecting and hydrothermal activities related to faulting and overpressure.     

Consolidation and creep behaviors of two typical marine clays in China

This paper presents an experimental investigation into the deformation characteristics of two typical marine clays obtained from Dalian and Shanghai, respectively, in China. Three kinds of laboratory tests, i.e. conventional oedometer tests, one-dimensional and triaxial creep tests were carried out. The results obtained from consolidation tests demonstrate linear v e ？ log？ relationships for Shanghai clay at normally consolidated state, while partly or even global non-linearrelationships for Dalian clay. The compression index c C for both clays follows the correlation of Cc=0.009（WL-10）where WL is the liquid limit of soil. The relationship between v log Kv （ Kv is the hydraulic conductivity of soil） and voidratio e is generally linear and the hydraulic conductivity change index kv C can be described by their initial void ratio forboth clays. The secondary compressibility of Dalian clay lies in medium to high range and is higher than that of Shanghaiclay which lies in the range of low to medium. Furthermore, based on drained triaxial creep tests, the stress-strain-timerelationships following Mesri＇s creep equation have been developed for Dalian and Shanghai clays which can predict thelong-term deformation of both clays reasonably well.     

鲜浒苔稀硫酸水解产糖工艺研究

分别用质量分数为0.6%、1.0%、1.4%、1.8%、2.2%的稀硫酸在121℃高温高压的条件下水解鲜浒苔30、60、90 min,浒苔水解残留物再用纤维素酶酶解,用DNS法测定上述各步骤中的还原糖产量。结果表明,浒苔总糖含量为67.2%。稀硫酸水解工艺中,硫酸质量分数为1.8%、水解90 min,浒苔还原糖转化率最高,达59.9%,每克干质量浒苔可产生447.0 mg还原糖。稀硫酸水解残留物纤维素酶酶解工艺中,浒苔还原糖转化率较低,在硫酸质量分数为0.6%、水解时间为60 min时还原糖转化率出现最大值,仅为6.5%。结果显示浒苔稀硫酸水解产还原糖效果显著,但并不能促进纤维素酶酶解。浒苔糖化可单独采用稀硫酸水解工艺,本实验条件下的最佳工艺条件为1.8%硫酸水解90 min。本研究探索了浒苔糖化工艺条件,对后续的浒苔燃料乙醇发酵研究具有一定的参考意义。     

基于PIV技术不同形式防波堤周围涡旋场特性研究(英文)

深水防波堤周围流场存在复杂的紊动变化,防波堤较容易发生失效破坏。本文利用PIV技术,基于模型实验,对规则波作用下防波堤周围的速度流场及其涡流特性进行了实验研究,获得了规则波流场的瞬时速度分布。通过对不同型式防波堤在不同的水力条件下的波浪流场实验数据的分析,定量地研究了防波堤堤身水域的涡旋流场的水力特性。     

基于特征区域统计的多光谱遥感影像海岛瞬时岸线提取(英文)

本研究采用IKONOS遥感图像,选取波谱特征区,通过区域像元统计并计算各波段权重系数,将影像进行波谱归一化处理,使归一化的影像岛陆与海水特征更明显。在此基础上,采用最大类间方差法确定最佳分割阈值,对归一化的影像进行二值化,从而提取海岛岸线。该方法采用特征曲线法进行水陆分离,得到的二值图像保持了原图的有效边缘,采用二值形态学提取的海岸线连续可靠、信噪比高。结果表明,该方法简单、快速,能有效提取海岸线,具有实用价值。     

基于码率控制的DMB预警信息预处理系统

为解决预警信息音频广播的单一性,通过对数字多媒体广播(DMB)的标准研究,设计了基于码率控制的DMB预警信息预处理系统.该系统采用MPEG-4 HE AAC和H.264/AVC编码算法对预警音视频信息分别进行信源编码,实现将编码后信息合成为TS码流;采用RS和卷积交织的信道编码方法实现TS码流的纠错控制,提高传输可靠性.针对传输码率不稳定、音视频传输容量和速率不匹配的技术难点,提出基于码率控制的音视频匹配控制传输方法,通过空包插入法实现对编码输出数据码率的智能控制,达到音视频预警信息的稳定实时输出.该预处理系统目前已通过验证,可显著提高预警信息传输的可靠性、稳定性和实时性.     

多旋翼无人机群自主探测大气边界层气象要素的模式分析

深入研究大气边界层气象现象的发生、发展、消散机制,需要获取三维同步气象要素数据,而现有探测技术与手段难以提供.因此,提出利用多旋翼无人机群进行自主探测大气边界层气象要素的探测模式,并就探测模式的背景与意义、特点与优势、流程与可行性,以及需要进一步研究的相关科学问题进行了分析,认为多旋翼无人机群自主探测大气边界层气象要素的探测模式针对性、灵活性、机动性、可操作性强,并具有自主动态同步探测、自主智能追踪探测等功能.