琼胶降解菌F-6筛选、培养条件及对条斑紫菜(Porphyra yezoensis)细胞解离作用的研究

利用琼胶作为唯一的碳源从青岛太平角海域的海水和红藻样品中筛选分离得到一株高产琼胶酶的海洋菌F-6,对其最适的产酶条件,利用琼胶酶分离条斑紫菜的原生质体和原生质体的培养进行研究。结果表明,通过单次单因子和正交实验确定琼胶降解菌株F-6最适产酶培养基配方为(W/V):琼胶0.7%;酵母粉0.3%;蛋白胨0.5%;NaCl2.0%;K2HPO40.1%;CaCl20.02%;MgSO4·7H2O0.05%;FeSO4·7H2O0.002%;起始pH=7.5,最适的发酵产酶条件为:26℃培养36h。经条件优化后,粗酶液的酶活高达631.6U/ml。发酵液经过6000g离心30min得到粗酶液,粗酶液经过8k分离膜超滤,加入1mol/L渗透剂,0.22μm滤膜过滤除菌后降解条斑紫菜组织块,成功地分离得到大量的紫菜原生质体,其产率为3×106个原生质体/g新鲜紫菜组织。原生质体经初步培养发育成愈伤组织,其成活率为60%。     

山东蓬莱大黑山岛碱性火山岩地球化学特征

庙岛群岛中的大黑山岛出露碱性火山岩,对研究壳-幔过程及黄、渤海演化具重要意义。文中作者对大黑山岛火山岩的常、微量元素、K-Ar年龄及Nd-Sr-Pb同位素体系进行测试确定。结果表明,岩石属钠质碱性玄武岩系列,岩浆岩喷发可分为8Ma前和8Ma后两个阶段;岩石常量元素含量除K2O、Na2O外差异小;随着火山岩的演化,岩浆源区深度变大;大黑山火山岩Nd-Sr-Pb同位素比值与夏威夷洋岛玄武岩相似,其岩浆的地幔端元组分为PREMA DM,又以PREMA为主,并有MORB物质混入。     

“数字海底”系统建模中的关键技术研究

基于“数字海底”系统的理论基础研究和在海底科学应用中的具体实践,提出了“数字海底”的涵义、系统组成和体系结构,对空间数据管理技术、基于XML的WebGIS空间数据表达技术、复杂海底三维建模技术、多源信息的一体化集成显示技术、虚拟现实技术等“数字海底”系统建模中的关键技术进行了探讨,为从事海底资源研究和开发的人员提供各个层次的参考或帮助.     

Dynamic Analysis of A Pontoon-Separated Floating Bridge Subjected to A Moving Load

1 .Introduction Since the most significant feature of a movingloadisits mobility,the interaction betweenthe ve-hicle and bridge is very complicated,which can be classified as a coupled vibration problem.There-fore ,much attention has been paid to the dyna…     

Analysis of Dynamic Characteristics of Submarine Free Spanning Pipelines by Complex Damping Method

Considering the effect of the internal flowing fluid and the external marine environmental condition, the differential equation for the vortex-induced vibration (V1V) of the free spanning pipeline is derived and is discretized by the Hermit interpolation function. The free vibration equation with the damping term is solved by the complex damping method for the natural frequency, and then the effect of fluid damping on the natural frequency of the free spanning pipeline is analyzed.The results show that fluid damping has a significant influence on the damped natural frequency of the free spanning pipeline in the lock-in state, while it has little influence when the pipeline is out of the lock-in state. In the meantime,the change of the free span length has the same effect on the damped natural frequency and the undamped natural frequency.     

全球船舶压载水管理项目在我国启动

为了防止全球船舶压舱水中的有害水生物在海洋中传播,危害海洋生态环境,造成海洋污染,2000年9月20日～21日,我国召开了关于制定船舶压载水管理国家项目工作计划和宣传项目研讨会。据悉,国际海事组织近年来一直在努力采取措施,制订有关法规,并将在2002年召开大会予以通过。届时,关于船舶压载水的管理规定将成为全球强制性要求。     

Geochemistry and environment evolution of Core E in the Laizhou Bay since last stage of Middle Pleistocene

IwrincrIONOn the evolution of palcoenvironment, salt-water encroachmnt and the distributionof subsurface brine in the LaizhOu Bay area, many researches had been carried out, and muchknowledge abeut the gcolOgical features in this area since Late QUaternary was aCquired' ) (H8nand Wu, l992; Han and Meng, l994; Wen et al. l989). However there was IittIe studyon the sediment geOChemical characteristics since Quaternary. In this Paper, the element strati-graphic characteristics and evolut…     

The Effect of Internal Fluid on the Response of Vortex-Induced Vibration of Marine Risers

Based on Iwan‘s wake oseillator model developed with the classical van der Pol equalion, the differential equation for the response of the vortex-induced vihration (VIV) of the riser considering the effect of the internal flowing fluid and the external marine environmental condition is derived. The effect of the intermal flowing fluid on the response of VIV of the riser is studied by means of the Finite Element Method. The results show that the effect of the internal fluid velocity on the VIV of the riser is strong when the natural frequency of the riser is close to the vortex shedding frequency. In addition, the increase of the top tension can decrease the sensitivity of the riser to the internal fluid velocity.     

晚更新世末期东亚季风活动与陆架区沉积环境变迁

从距今 18 0 0 0年的最后冰期最盛时期到距今 10 0 0 0年开始的全新世海侵 ,全球气侯发生过急剧的变化。位于亚洲东部的古季风活动也经历了盛衰变化。大量的钻孔及浅地层剖面分析资料已经证实 ,冰期最盛时期陆架区古季风活动加强 ,陆架出现了沙漠环境与黄土沉积。当冰期结束 ,气候转暖 ,冬季风减弱之际 ,南黄海陆架及长江三角洲一带的沙漠与黄土分布范围也随之缩小 ,原先的沙漠活动区变成了黄土分布区 ,也就是所谓硬粘土沉积区。随着古季风活动的进一步衰退和海面的不断升高 ,来自陆架区的东北风变为冷湿气流。长江三角洲地区和陆架上出现了有利于沼泽发育的环境 ,全新世海侵把泥炭层淹没。陆架区晚更新世末期以来的地层中出现沙层、硬粘土和泥炭层的沉积序列 ,即“三元沉积结构”,是古冬季风盛衰过程的最好记录     

星载SAR浅海水下地形和水深测量模拟仿真──水下地形高度、坡度和方向与可测水深分析

根据星载合成孔径雷达(SAR)浅海水下地形和水深成像机理,建立了浅海水下地形和水深雷达后向散射截面仿真模型.利用该模型模拟并分析了不同地形条件下,浅海水下地形的雷达后向散射截面.分析结果表明,水下地形高度越高,SAR可测量的水深越深;水下地形坡度越大,越易被SAR所观测.水下地形的星载SAR测量还与水下地形的方向有关,与卫星飞行方向平行的水下地形最易被SAR观测,与卫星飞行方向垂直的水下地形最不易被SAR观测.