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21.
Barley(Hordeum vulgare L.) is one of the earliest domesticated crop species and ranked as the fourth largest cereal production worldwide. Forward genetic studies in barley have greatly advanced plant genetics during the last century; however, most genes are identified by the conventional mapping method. Array genotyping and exome-capture sequencing have also been successfully used to target the causal mutation in barley populations, but these techniques are not widely adopted because of associated costs and partly due to the huge genome size of barley. This review summarizes three mapping cases of barley cuticle mutants in our laboratory with the help of RNA-sequencing. The causal mutations have been successfully identified for two of them and the target genes are located in the pericentromeric regions. Detailed information on the mapping-by-sequencing, mapping-and-sequencing, and RNA-sequencing assisted linkage mapping are presented and some limitations and challenges on the mapping assisted by RNA sequencing are also discussed. The alternative and elegant methods presented in this review may greatly accelerate forward genetics of barley mapping, especially for laboratories without large funding. 相似文献
22.
基于生态敏感性-生态恢复力-生态压力度(SRP)概念模型,从地形、气候、植被和社会经济 因子选取 8 个评价指标,利用遥感和 GIS 技术,采用主成分分析方法求取权重,对祁连山地区启动 水源涵养区生态环境保护和综合治理规划研究前后近 10 a 的生态脆弱性程度进行系统、定量地评 估,旨在揭示生态脆弱性的分布特征、时空演变及动因,为区域生态保护、资源利用和可持续发展 提供参考。结果表明:(1)从研究区生态脆弱性分布来看,祁连山地区主要以轻度和重度脆弱为 主,脆弱性程度从西北向东南地区逐渐减弱,西北地区植被覆盖度小,海拔高,生态环境较为恶劣 是导致脆弱性程度较高的原因;(2)祁连山地区 3 期生态脆弱程度呈逐渐下降趋势,综合指数分别 为 3.307、3.118 和 3.103;2005 年 生 态 脆 弱 性 较 高 ,极 度 脆 弱 面 积 为 28 610 km2,2010 年 下 降 为 11 723 km2,2015 年降低为 6 174 km2,极度脆弱面积逐渐减少;(3)从祁连山地区生态脆弱性演变动 因来看,8 个指标对生态脆弱性影响均较为显著,但在不同的时间影响程度各不相同,2005—2015 年 3 期数据中对生态脆弱性影响最大的均为植被指数,降水次之,地形因子影响最小。总体来看, 近年来祁连山地区生态脆弱性程度有所降低,但仍然需要加强保护力度,促进生态环境可持续 发展。 相似文献
23.
Microbial degradation technologies have been developed to restore ground water quality in aquifers polluted by organic contaminants effectively in recent years. However, in course of the degradation, the formation of biofilms in ground water remediation technology can be detrimental to the effectiveness of a ground water remediation project. Several alternatives are available to a remedial design engineer, such as Permeable Reactive Barriers (PRBs) and in -situ bioremediation, Hydrogen Releasing Compounds (HRCs) barrier, Oxygen Releasing Compounds (ORCs) barrier etc. which are efficient and cost- effective technologies. Excessive biomass formation renders a barrier ineffective in degrading the contaminants, Efforts are made to develop kinetics models which accurately determine bio - fouling and bio - filn formation and to control excessive biomass formation. 相似文献
24.
私营企业主是我们社会主义市场经济的建设者 ,是否“合法”是我们正确解读私营企业主的关键。我国目前的私营企业主分为两类三种。合法经营的私营企业主中的优秀分子是吸收入党的主要对象 相似文献
25.
A CASE STUDY ON CONCENTRATION AND DECENTRALIZATION:BEHAVIOR AND DYNAMIC MECHANISM OF SPATIAL EVOLUTION IN METROPOLITAN AREA,NANJING,CHINA 总被引:1,自引:1,他引:0
In rapid socio-economic development,the process of concentration and dispersal of various elements tends to be more dramatic,tremendously influencing the shaping and transformation of the space in metropolitan area.Survey of spatial concentration and decentralization has thus become a basic method in examining metropolitan spatial evolution.In this research,three elements were selected as the essential indicators of the process:demographic density distribu-tion,employment density distribution and business office location.Performance of these elements in Nanjing City was exam-ined historically.As Nanjing City could be regarded as a representative of metropolitan areas in China,its situation large-ly suggestes the general characteristics in similar areas of China.Hence based on the investigation of Nanjing City,four general implications were highlighted.First ,metropolitan areas in China are in a violent process and shift of spatial concentra-tion and decentralization.Second,from now to at least the near future,concentration will continue to be the central fea-ture.Third,the landscape of metropolitan areas basically exhibits a dual structure character.The gap in environmental and ecological qualities among different districts will continue for a long time.Fourth,Central Business District (CBD) is playing an important role in helping to convert the traditionally single-centered city structure into a polycentric one. 相似文献
26.
青藏高原隆升的非线性动态有限元仿真研究 总被引:7,自引:4,他引:3
根据青藏高原的地质特征建立分析模型,采用3维动态有限元方法,在计算仿真板块速度场的基础上,计算在青藏高原的隆升过程中该地区地壳岩石的等效应力和位移随时间的变化,计算仿真得到的速度场与1998年GPS观测的速度场吻合良好;与过去一贯的假设相反,计算结果反映出地壳应力场不是静态的,而是此起彼伏,不断变化的,应力值最大且变化最剧烈的地区在克什米尔地区、鄂尔多斯地区和鲜水河-小江断裂带,与地震多发区域吻合。 相似文献
27.
对青藏东北缘现今块体划分、运动及变形的初步研究 总被引:19,自引:9,他引:10
利用2维非连续变形分析方法(DDA),以位移代替围压作为边界约束力,研究青藏东北缘现今块体划分及其运动变形。根据该地区地质构造及地震活动,以GPS点测量位移作为模拟结果约束点,得出了较合理的块体划分模型和随时间演化的主应变分布图,并把应变高值区与近几年来发生的5级以上地震作对比,得出了研究区内地震危险性可能较大的区域。另外,对模拟的甘青块体与阿拉善块体的边缘带断裂左旋运动做了大概计算。 相似文献
28.
高速滑坡问题是当前工程地质和环境地质学界极为关注的问题。本文对诸如滑体高速滑动所对应的时空界限、滑体滑动过程所产生的热效应以及空气浮托力等高速滑坡产生机制问题进行了初步探讨。 相似文献
29.
A wide-field time-series CCD photometric survey of variable stars in the field of the open cluster NGC 2168 was carried out using the BATC Schmidt telescope. In total 13 new variable stars are discovered with three W UMa systems, one EA type and two EB type eclipsing binaries (one of them could be a W UMa system), and seven pulsating stars including three candidates of δScuti stars. 相似文献
30.
Xue-Fei Chen Zhan-Wen HanNational Astronomical Observatories Yunnan Observatory Chinese Academy of Sciences. Kunming Graduate School of the Chinese Academy of Sciences Beijing 《中国天文和天体物理学报》2005,5(1):65-76
We have studied the influence of different choices of core-envelope transition point on the final merger of contact binaries with two main-sequence components. A binary of 1.00 + 0.90M⊙ with an initial orbital period of 0.35d is examined. The mass fraction of the primary mixed with the matter of the secondary, qmix, determined by the chosen core-envelope transition point, ranges from 0.04 to 1.00 in our analysis. If as qmix< 0.8, none of the 相似文献