Isolation,biodegradation ability and molecular detection of hydrocarbon degrading bacteria in petroleum samples from a Brazilian offshore basin

The detection of microorganisms with potential for biodeterioration and biodegradation in petroleum fields is of great relevance, since these organisms may be related to a decrease in petroleum quality in the reservoirs or damage in the production facilities. In this sense, petroleum formation water and oil samples were collected from the Campos Basin, Brazil, with the aim of isolating microorganisms and evaluating their ability to degrade distinct classes of hydrocarbon biomarkers (9,10-dihydrophenanthrene, phytane, nonadecanoic acid and 5α-cholestane). Twenty eight bacterial isolates were recovered and identified by sequencing their 16S rRNA genes. Biodegradation assays revealed that bacterial metabolism of hydrocarbons occurred through reactions based on oxidation, carbon–carbon bond cleavage and generation of new bonds or by the physical incorporation of hydrocarbons into microbial cell walls. Based on the biodegradation results, selective PCR-based systems were developed for direct detection in petroleum samples of bacterial groups of interest, namely Bacillus spp., Micrococcus spp., Achromobacter xylosoxidans, Dietzia spp. and Bacillus pumilus. Primer sets targeting 16S rRNA genes were designed and their specificity was confirmed in silico (i.e. computational analysis) and in PCR reactions using DNA from reference strains as positive and negative controls. Total DNA from oil was purified and the amplification tests revealed the presence of the target bacteria in the samples, unraveling a significant potential for petroleum deterioration in the reservoirs sampled, once proper conditions are present for hydrocarbon degradation. The application of molecular methods for rapid detection of specific microorganisms in environmental samples would be valuable as a supporting tool for the evaluation of oil quality in production reservoirs.     

Water Availability of Sub-Basins with Restrictions on Use under Climate Change Scenarios in Brazil

Water Resources - The Queima Pé Stream Basin (QPSB) and Ararão River Basin (ARB) in central Brazil have had recurrent problems with water scarcity. This may get worse under anthropogenic...     

Camacho inlet southern Brazil - Instability causes and user conflicts

Inlet stabilization is a common practice in Brazil, as it is worldwide. In this paper we present results of the analysis of the stabilization practices of the Camacho Inlet, located in Santa Catarina State, southern Brazil. This inlet is maintained intermittently, in accordance with a regime designed to benefit both local fishermen and rice farmers. Fishermen require this inlet to be open as a navigation channel during the fishing season; rice farmers needs it closed during the irrigation period to allow the pumping of fresh water from the Congonhas River which drains into the Camacho’s backbarrier lagoon. An additional complication arises from the tendency of this inlet to close naturally due to infilling by dune migration. Aerial images spanning a 65 year time period (1938-2003) were analyzed, demonstrating the variability in the inlet’s width between 0 (closed) and 360 m. The analysis of inlet stability and user needs indicate that an appropriate solution is the implementation of an annual inlet dredging program in the austral autumn. This would allow for the inlet to remain open from May to September and then close naturally for the rest of the year, providing for a backbarrier fresh water reservoir during the irrigation period.     

Binary collisions and the slingshot effect

We derive the equations for the gravity assist manoeuvre in the general 2D case without the constraints of circular planetary orbits or widely different masses as assumed by Broucke (AIAA/AAS 1988) and obtain the slingshot conditions and maximum energy gain for arbitrary mass ratios of two colliding rigid bodies. Using the geometric view developed in an earlier paper by the authors (Rica da Silva, A., Lemos, J.P.S.: Am. J. Phys. 74, 584–590, 2006) the possible trajectories are computed for both attractive or repulsive interactions yielding a further insight on the slingshot mechanics and its parametrization. . The general slingshot manoeuvre for arbitrary masses is explained as a particular case of the possible outcomes of attractive or repulsive binary collisions, and the correlation between asymptotic information and orbital parameters is obtained in general.     

S?o Francisco-Congo Craton break-up delimited by U-Pb-Hf isotopes and trace-elements of zircon from metasediments of the Ara?uaí Belt

Detrital zircon U-Pb geochronology combined with Hf isotopic and trace element data from metasedimentary rocks of the Aracuai Belt in southeastern Brazil provide evidence for break-up of the Congo-Sao Francisco Craton. The U-Pb age spectra of detrital zircons from metasediments of the Rio Doce Group(RDG) range from 900-650 Ma and define a maximum depositional age of ca. 650 Ma. Zircon trace element and whole rock data constrain an oceanic island arc as source for the deposition setting of the protoliths to the metasediments. Zircon ε_(Hf)(t) values from these rocks are positive between +1 and +15, supporting previous evidence of a Neoproterozoic extensional phase and oceanic crust formation in a precursor basin to the Aracuai Belt. Recrystallization of detrital zircon at ca. 630 Ma is compatible with a regional metamorphic event associated with terrane accretion to the Paleoproterozoic basement after transition from an extensional to a convergent regime. The juvenile nature, age spectra and trace element composition recorded in detrital zircons of metasediments from the Aracuai Belt correspond with zircons from metasedimentary rocks and oceanic crust remnants of other orogenic belts to its south. This suggests that rifting and oceanic crust formation of the entire orogenic system, the so-called Mantiqueira Province, was contemporaneous, most likely related to the opening of a large ocean. It further indicates that the cratonic blocks involved in the orogenic evolution of the Mantiqueira Province were spatially connected as early as 900 Ma.     

Correction to: Spatial distribution and estimation of rainfall trends and erosivity in the Epitácio Pessoa reservoir catchment,Paraíba,Brazil

Natural Hazards - The article “Spatial distribution and estimation of rainfall trends and erosivity in the Epitácio Pessoa reservoir catchment, Paraíba, Brazil”, written by da...     

Urban forest loss using a GIS-based approach and instruments for integrated urban planning:A case study of Jo(a)o Pessoa,Brazil

Urban forests play an important role in the thermal comfort and overall life of local populations in large-and medium-sized cities.This study analyzes urban forest loss and maps land use and land cover (LULC) changes between 1991 and 2018 by evaluating the use of urban planning instruments for the mitigation of urban forest loss in Jo～o Pessoa,Brazil.For this purpose,satellite-derived LULC images from 1991,2006,2010 and 2018 and data on urban forest loss areas obtained using the Google Earth Engine were used.In addi-tion,this paper also discusses the instruments used for integrated urban planning,which are(a) the legal sector,responsibility and nature;(b) the urban expansion process;and (c) the elements of urban infrastructure.The results show a clear shift in land use in the study area.The major changes in LULC classes occurred in urban areas and herbaceous vegetation,while the greatest loss was in arboreal/shrub vegetation.Thus,an increase in the pressure to occupy zones intended for environmental preservation could be estimated.Our results showed similar accuracies with other studies and more spatial details.The characteristics of the patterns,traces,and hotspots of urban expansion and forest cover loss were explored.We highlighted the potential use of this proposed framework to be applied and validated in other parts of the world to help better understand and quantify various aspects of ur-ban-related problems such as urban forest loss mapping using instruments for integrated urban planning and low-cost approaches.     

Impacts of climate change on building energy demands in the intra-Americas region

It was recently reported a regional warming in the intra-Americas region where sea surface temperature exhibited increases exceeding 0.15 °C/decade and an accelerated air temperature rise that could impact building energy demands per capita (EDC). Reanalysis data is used herein to quantify the impacts of these warming trends on EDC. Results of the analysis depict a Southern Greater Antilles and inland South America with a positive annual EDC rate of 1–5 kWh per year. The Intergovernmental Panel on Climate Change (IPCC) Representative Concentration Pathways (RCP) 2.6 and 4.5 scenarios were selected to analyze energy demand changes in the twenty-first century. A multi-model ensemble forecasts an EDC increase of 9.6 and 23 kWh/month in the RCP2.6 and RCP4.5 at the end of the twenty-first century, which may increase average building cooling loads in the region by 7.57 GW (RCP2.6) and 8.15 GW (RCP4.5), respectively. Furthermore, 4 of 9 (RCP2.6) and 7 of 9 (RCP4.5) of the major countries in this region have EDCs ranging between 1887 and 2252 kWh/year at the end of this century. Therefore, increased energy production and improved energy infrastructure will be required to maintain ideal indoor building conditions at the end of the twenty-first century in these tropical coastal regions as consequence of a warmer climate.     

Flash flood in the mountainous region of Rio de Janeiro state (Brazil) in 2011: part I—calibration watershed through hydrological SMAP model

Natural Hazards - Flooding and water logging possess severe hazards to human population in many parts of the world. Mumbai, the study area, is one of the cities in India and has been frequently...