内蒙古科右前旗大石寨组火山岩锆石LA-ICP-MSU-Pb年龄及其形成背景

对大石寨组火山岩的形成时代和形成背景的认识存在较大的分歧.对采自建组剖面底部英安岩样品中的锆石进行阴极发光照相、微区原位LA-ICP-MS微量元素分析和U-Pb测年.锆石U-Pb同位素分析结果显示,锆石发育典型的岩浆震荡环带,高Th/U(0.51～1.57),轻稀土元素亏损,重稀土元素富集,具有强烈的正Ce异常和强烈的负Eu异常等特征,表明锆石均属于岩浆成因.22个分析点获得的谐和年龄为(314±1)Ma(MSWD=0.88),代表了大石寨组火山岩的形成时代.结合前人的资料,认为大石寨组火山岩为裂陷早期的产物,而晚古生代的沉积岩石组合为在这一基础上发展起来的具有裂陷盆地性质的沉积组合.     

深海沉积物宏基因组文库中产蛋白酶克隆CAPRO2的筛选及酶学性质分析

利用选择性筛选培养基从所构建的深海沉积物宏基因组文库中筛选得到一株产蛋白酶的克隆（CAPR0002）,对其进行了酶学性质分析．结果表明该酶的最适作用温度为65cc,最适作用pH值为9．0．该蛋白酶具有较好的热稳定性,在40cC以下的温度中可长期保持稳定,在50℃中处理6h后仍能保持60％的活力,在60℃下保温30rain后仍能保持约60％的活力．Ca^2＋、Mg^2＋、sr^2＋、co^2＋对该蛋白酶有明显的促进作用,而且ca^2＋的存在可明显提高该蛋白酶的热稳定性,ca^2＋、sr^2＋、c0^2＋这3种离子均在3．0mmol／dm^3。浓度时具有最高的促进作用,当浓度高于3．0mmol／dm’时,这3种离子对酶活力的促进作用减弱．Hg^2＋、Fe^2＋、cu¨对酶有明显的抑制作用．CAPR02蛋白酶在pH值为7。5～9．5时活力较高,pH值为7。5时可保持约80％的活力,pH值为9．5时保持60％的活力,而在pH值高于9．5的条件下酶活力下降较快,pH值为10．0时活力降为约15％,表明CAPR02属于碱性蛋白酶．丝氨酸蛋白酶抑制剂PMSF、E一64和AEBSF对CAPR02蛋白酶均无抑制作用,显示该酶不属于丝氨酸蛋白酶,而EDTA对酶有明显的抑制作用,表明该酶属于金属蛋白酶．     

氨氮胁迫对拟穴青蟹组织器官中SOD及GPX活性的影响

采用实验生态学的方法,探讨海水中分别添加氨氮（以氯化铵作为氨氮源）0（对照组,C0组）、10（C10组）、20（C20组）、30（C30组）、40 mg/dm3（C40组）,胁迫24、48、72、96 h对拟穴青蟹（Scylla paramamosain）各组织器官中SOD、GPX活性的影响.结果表明,拟穴青蟹鳃中SOD活性,除C40组外,其余各组在胁迫24、48 h随氨氮含量升高而升高,胁迫72、96 h则随氨氮含量升高而下降（p〈0.05）,C40组的则随胁迫时间延长而一直呈下降趋势;各组拟穴青蟹鳃、肝胰腺和肌肉的SOD活性变化趋势相同,鳃和肌肉SOD活性最高均出现在胁迫48 h,肝胰腺SOD活性最高则在胁迫72 h.各组拟穴青蟹鳃GPX活性在胁迫24、48和72 h随氨氮含量升高而下降,胁迫96 h随氨氮含量升高而增大;各组拟穴青蟹肝胰腺和肌肉GPX活性变化与肝胰腺SOD活性变化趋势相似,胁迫48 h,C40组拟穴青蟹肝胰腺GPX活性最高.氨氮胁迫显著影响拟穴青蟹生理效应.     

夏季长江口浮游细菌和浮游病毒的分布特征及环境制约因素

应用荧光显微直接计数法,研究了2006年夏季长江口及邻近海域浮游细菌、浮游病毒数量的分布特征,探讨了它们与环境因子之间的关系.结果表明:(1)浮游细菌数量为(6.92×105～5.54×106)个/mL,浮游病毒数量为(2.22×106～9.97×107)个/mL.浮游细菌和浮游病毒数量的平面分布特征较一致,均为近海过...     

深海平台组块运输船结构及其可靠性研究

根据我国海洋油气开发的需要,研究出一种可用于深海的大型构件、平台组块运输的新型甲板运输船.文中着重对其总纵强度和横向强度进行深入分析,优化出可满足任何海域的结构形式,并在此基础上进行可靠性的研究.     

偕胺肟基聚丙烯腈/蒙脱土纳米复合材料海水铀的吸附规律研究

采用偕胺肟化聚丙烯腈/蒙脱土(APAN/MMT)复合纳米吸附材料对海水中的铀进行吸附,考察了吸附条件对APAN/MMT吸附铀量的影响,并对APAN/MMT吸附铀的动力学和热力学进行了探讨.结果表明:铀初始浓度、溶液温度较高、采用磁力搅拌吸附方式有利于吸附速率的提高,溶液pH对吸附性能有一定的影响,在pH为5时,平衡吸附...     

Estimation of land production and its response to cultivated land conversion in North China Plain

Food safety and its related influencing factors in China are the hot research topics currently, and cultivated land conversion is one of the significant factors influencing food safety in China. Taking the North China Plain as the study area, this paper examines the changes of cultivated land area using satellite images, estimates land productivity from 1985 to 2005 using the model of Estimation System for Land Productivity (ESLP), and analyzes the impact of cultivated land conversion on the land production. Compared with the grain yield data from statistical yearbooks, the results indicate that ESLP model is an effective tool for estimating land productivity. Land productivity in the North China Plain showed a slight decreasing trend from 1985 to 2005, spatially, increased from the north to the south gradually, and the net changes varied in different areas. Cultivated land area recorded a marginal decrease of 8.0 × 10⁵ ha, mainly converted to other land uses. Cultivated land conversion had more significant negative impacts on land production than land productivity did. Land production decreased by about 6.48 × 10⁶ t caused by cultivated land conversion between 1985 and 2005, accounting for 91.9% of the total land production reduction. Although the land productivity increased in Anhui and Jiangsu provinces, it can not offset the overall adverse effects caused by cultivated land conversion. Therefore, there are significant meanings to control the cultivated land conversion and improve the land productivity for ensuring the land production in the North China Plain.     

Cold-adaptive alkaline protease from the psychrophilic Planomicrobium sp.547: enzyme characterization and gene cloning

A psychrophilic bacterium strain 547 producing cold-adaptive alkaline protease was isolated from the deep sea sediment of Prydz Bay, Antarctica. The organism was identified as a Planomicrobium species by 16S rRNA analysis. The optimal and highest growth temperatures for strain 547 were 15℃ and 30℃, respectively. The extracellular protease was purified by ammonium sulfate precipitation and DEAE cellulose-52 chromatography. The optimal temperature and pH for the activity of the purified enzyme were 35 ℃ and pH 9.0, respectively. The enzyme retained approximately 40% of its activity after 2 h of incubation at 50℃. The enzymatic activity was inhibited by 1 mmol/L phenylmethyl sulfonylfluoride (PMSF) and hydrochloride 4-(2-aminoethyl)-benzenesulfonyl fluoride (AEBSF), indicating that it was a serine protease. The presence of Ca2+ and Mn2+ increased the activity of the enzyme. The protease gene with a size of 1 269 bp was cloned from Planomicrobium sp. 547 using primers designed based on the conserved sequences of proteases in GenBank. The Planomicrobium sp. 547 protease contained a domain belonging to the peptidase S8 family, which has a length of 309 amino acid (AA) residues. The alignment and phylogenetic analysis of the AA sequence indicated that the protease belonged to the subtilisin family.     

巴基斯坦红其拉甫国家公园种子植物区系分析

红其拉甫国家公园(国家自然保护区)位于巴基斯坦北部地区,喀喇昆仑山南坡,介于36°04′～37°05′N和74°38′～75°45′E之间,海拔3 200～6 000 m之间,平均海拔4 000 m,总面积约2 270 km2,建于1975年.通过实地考察和查阅相关文献资料,对分布于该地区的植物从区系组成、区系性质的角...     

相控阵天气雷达监测预警龙卷风的过程剖析

天气雷达作为龙卷风监测预警的重要手段之一,应用具有超高时空分辨率的X波段双极化相控阵天气雷达系统,较好地捕获并提前预警龙卷风。以2022年6月19日07时发生在广东佛山南海的一次龙卷风为例,详细剖析龙卷生消及雷达监测预警过程。借助雷达智能预警软件,利用X波段双极化相控阵天气雷达的双偏振量和超高时空分辨率数据,实时反演三维风场和分析龙卷碎片(TVS)特征,能够显著提高龙卷风监测预警水平。实例表明,本次成功地提前18分钟预警龙卷,进一步说明了X波段双极化相控阵天气雷达在强对流天气探测方面具有较强的生命力。