ECOLOGICAL DISTRIBUTION OF HYDROCARBON-DEGRADATION BACTERIA IN XIAMEN HARBOR

The ecology of hydrocarbon-degrading bacteria were investigated during five cruises in Xiamen Harbor. The results demonstrated that number of hydrocarbon-degrading bacteria were 2.1×102-7.5×106 cell/1 and 1.7×102-1.5×106 ceil/g dry and hetcrotrophic bacteria were 3.0× 104-5.9× 109 cell/1 and 2.53× 103-5.0× 103 cell/g dry in seawater and sediment respectively. The isolated strains which can degrade the petroleum belong to fifteen genera. Most, strains can only degsade one kind of hydro- carbon or petroleum. The results showed that the population and the species-composition of hydro carbon-degrading microorganisms were positively correlated with existing level of oil pollution and with water temperature, but independent of total microbtat count.     

1998年春深圳湾环节环沟藻赤潮及其发生原因的探讨

1998年4月23日至29日深圳湾发生了较大规模的环节环沟藻赤潮,面积逾200km2,细胞密度高达3.8×106 个/L。赤潮发生于大量降雨之后 ,高峰期水体盐度在20以下。在深圳河河口海域 ,虽然富营养化程度也较高 ,但由于悬浮物含量过高 ,浮游植物及环节环沟藻数量较少。赤潮期间 ,深圳湾没有发生死鱼现象 ,养殖牡蛎的大量死亡估计与赤潮无关     

CO<Subscript>2</Subscript> Flux Measurements over the Sea Surface by Eddy Correlation and Aerodynamic Techniques

The measurements of the vertical transport of CO₂ were carried out over the Sea of Japan using the specially designed pier of Kyoto University on September 20 to 22, 2000. CO₂ fluxes were measured by the eddy correlation and aerodynamic techniques. Both techniques showed comparable CO₂ fluxes during sea breeze conditions: −0.001 to −0.08 mg m⁻²s⁻¹ with the mean of −0.05 mg m⁻²s⁻¹. This means that the measuring site satisfies the fetch requirement for meteorological observations under sea breeze conditions. Moreover, the eddy diffusivity coefficient used in the aerodynamic technique is found to be consistent with the coefficient used in the eddy correlation technique. The present result leads us to conclude that the aerodynamic technique may be applicable to underway CO₂ flux measurements over the ocean and may be used in place of the bulk technique. The important point is the need to maintain a measuring accuracy of CO₂ concentration difference of the order of 0.1 ppmv on the research vessels or the buoys.     

Roles of Biogeochemical Processes in the Oceanic Carbon Cycle Described with a Simple Coupled Physical-Biogeochemical Model

To evaluate the contribution of biogeochemical processes to the oceanic carbon cycle and to calculate the ratio of calcium carbonate to organic carbon downward export, we have incorporated biological and alkalinity pumps in the yoked high-latitude exchange/interior diffusion-advection (YOLDA) model. The biogeochemical processes are represented by four parameters. The values of the parameters are tuned so that the model can reproduce the observed phosphate and alkalinity distributions in each oceanic region. The sensitivity of the model to the biogeochemical parameters shows that biological production rates in the euphotic zone and decomposition depths of particulate matters significantly influence horizontal and vertical distributions of biogeochemical substances. The modeled vertical fluxes of particulate organic phosphorus and calcium carbonate are converted to vertical carbon fluxes by the biological pump and the alkalinity pump, respectively. The downward carbon flux from the surface layer to the deep layer in the entire region is estimated to be 3.36 PgC/yr, which consists of 2.93 PgC/yr from the biological pump and 0.43 PgC/yr from the alkalinity pump, which is consistent with previous studies. The modeled rain ratio is higher with depth and higher in the Pacific and Indian Oceans than in the Atlantic Ocean. The global rain ratio at the surface layer is calculated to be 0.14 to 0.15. This value lies between the lower and higher ends of the previous estimates, which range widely from 0.05 to 0.25. This study indicates that the rain ratio is unlikely to be higher than 0.15, at least in the surface waters.     

Long-Term Investigation of Spatio-Temporal Variations in Faunal Composition and Species Richness of Megabenthos in Ise Bay,Central Japan

Based on our long-term data from megabenthos sampling from 1993 to 2002 in Ise Bay, central Japan, we examined spatio-temporal variations in taxon composition, species richness and its distribution of megabenthos in the bay in relation to the occurrence of the oxygen-poor water (i.e. oxygen content less than 3 ppm) in bottom waters of the bay. A total of 261 species were identified including 6 cnidarians, 1 tentaculate, 5 annelids, 71 molluscs, 72 crustaceans, 16 echinoderms, 12 urochordates and 78 pisces. Of the most abundant 10 megabenthos species, the following 4 species of echinoderms made up more than the half of megabenthos biomass: Luidia quinaria, Echinocardium cordatum, Asterias amurensis and Astropecten scoparius. Species richness of megabenthos varied significantly between seasons and among stations. The severity and period of occurrence of the oxygen-poor water developing every summer play an important role in determining spatial distributions of species richness in the bay.     

合浦珠母贝26S蛋白酶体S4,S7亚基基因片段的分离与分析

26S蛋白酶体是真核生物中一种具有ATP依赖性的蛋白酶复合体,主要通过泛肽途径选择性降解细胞内与代谢调控、细胞周期有关的功能蛋白及异常蛋白,参与多种细胞活动的调控过程。26S蛋白酶体由具有催化活性的20S亚复合体和一个具有调节作用的19S亚复合体组成,其中19S亚复合体中的ATP酶亚基是调节26S蛋白酶体活性的重要组件。本通过简并引物PCR手段,从软体动物合浦珠母贝(Pinctada fucata)中扩增到参与构成19S亚复合体的S4和S7(MSS1)两个亚基的基因片段。这两个基因片段所编码的ATP酶组件包含有Gx4GKT,DEID,SAT和H／QRxGRxxR等26S蛋白酶体ATP酶亚基的共同功能基序。这是首次在软体动物中报道26S蛋白酶体的ATP酶亚基基因序列,为研究软体动物中26S蛋白酶体的结构与功能奠定了分子基础。     

远区台风“三巴”对长江口波浪动力场的作用机制

针对远区台风对河口波浪动力场的影响问题,利用第三代波浪模式SWAN计算了远区台风"三巴"期间长江口波浪动力场分布,分析了陆架至河口区的波浪能量耗散和波致泥沙侵蚀的时空分布,发现波浪由外海向近岸传播过程中,波-波相互作用导致能量由高频向低频转换,周期和波长逐渐增大,近底层轨道流速增大,能量密度增高;阐明白帽破碎是维持深水区波浪能量平衡和限制波高成长的主要机制,底摩擦耗能和水深诱导的破碎耗能是长江口横沙东滩和崇明东滩邻近海域波高衰减的主要原因;提出波浪产生的底部切应力与相对水深有关,当波浪传播到浅水区时,波长和周期越大,波浪切应力越大。研究揭示了与河口相距数百公里的远区台风能够对长江口波浪动力场产生明显影响,河口水下三角洲前缘是最容易受到波浪侵蚀的区域,研究成果弥补了目前关于陆架远区台风对河口波浪动力场影响研究的不足,对深化认识远区台风对长江口动力环境、地貌演变、航运安全和滩涂保护等有重要科学意义。     

大黄鱼倍性检测研究

于2001—2004年对人工诱导大黄鱼的不同发育阶段和不同组织进行倍性检测，胚胎眼泡期采用染色体计数法测定，鱼苗和成鱼阶段用流式细胞法，结果表明，胚胎染色体数目2n=48条为二倍体，3n=72条为三倍体．采用流式细胞法测定鱼苗和成鱼个体时，若受测样品的DNA相对含量值与已知对照组二倍体的比值约为1．5：1，其个体为三倍体；比值约为1：1，则为二倍体，无论用肌肉或尾鳍组织，皆可检测出其个体的倍性。     

基于GIS的柘林湾生态信息系统

柘林湾生态信息系统以ArcGIS为开发平台，采用客户端／服务器的体系结构，基于ArcObjects的组件式二次开发而成，实现了柘林湾的三维实景模拟、生态数学模型与GIS的有效集成、分析评价结果与地理空间数据相结合等功能，具有可视化、智能化、易扩展、安全实用等特点．该系统的建成．极大地提高了柘林湾生态信息的现代化管理水平，并为制定柘林湾的污染防治与生态环境保护策略提供了强有力的支持．     

Relationship between Salinity and Nutrients in the Subsurface Layer in the Suruga Bay

We investigated the water structure and nutrient distribution in the Suruga Bay from April 2000 to July 2002, especially the Offshore Water, which occupies a large part of the bay. The maximum salinity in the upper 200 m varied between 34.49 and 34.71, indicating a temporal change in the influence of Kuroshio Water on the Offshore Water. Seasonal variation in nutrient concentrations was largest from surface to 50 m. On the other hand, the variance in nutrient concentrations within each season was largest in the subsurface layer of 100–300 m in spring, summer and fall. In the Offshore Water, the change of nutrients was negatively correlated with that of salinity in each season. This suggests that an increasing intrusion of saline water brings about a lower nutrient concentration in the Offshore Water. Likewise, negative correlations were observed between the change of the maximum salinity and chlorophyll a (Δ [chl.a-int])/nutrients integrated in the upper 200 m. Δ[chl.a-int] was significantly correlated with the changes of nitrate and phosphorus, but there were no significant correlations between Δ[chl.a-int] and the change of silicate. These results suggest that the concentrations of chlorophyll a and nutrients in the Offshore Water were decreased due to the increasing intrusion of Kuroshio Water. The Offshore Water is likely to be related to the regulation of primary production by nitrate.