海湾扇贝养殖群体遗传多样性的研究

用等位基因酶的聚丙烯酰胺凝胶电泳技术,研究了大连沿岸不同生态环境条件下的大李家湾低排筏、大李家湾高排筏、凌水桥、棋盘磨4个海湾扇贝养殖群体的遗传多样性.在4个群体的7种等位基因酶和闭壳肌蛋白中均检测到了22个基因位点,各群体的多态位点比例分别为22.73%,22.73%,22.73%,18.18%(P≤0.95)和27.27%,27.27%,31.82%,36.36%(P≤0.99);杂合度观测值分别为0.064,0.071,0.075,0.069;Hardy-Weinberg平衡偏离指数分别为-0.068,-0.020,-0.032,-0.050;近交系数分别为0.568,0.565,0.487,0.550;位点等位基因有效数分别为1.102,1.109,1.143,1.122;遗传变异综合评价指数分别为0.0060,0.0071,0.0088,0.0073.海湾扇贝养殖群体遗传多样性总体水平较低,但在这4个群体中,凌水桥群体的遗传多样性水平最高,大李家湾高排筏和棋盘磨群体的遗传多样性水平较高,大李家湾低排筏群体遗传多样性水平最低.     

AHP RESIN AND ITS URANIUM ADSORPTION MECHANISM

I. INTRODUCTION AHP resin is a new type of adsorbent and has been studied by the units concernedfor several years. There is little report about this work so far. AHP resin was synthesizedin aqueous phase with tetra-ethylene pentamine (TEPA), epichlorohydrin (ECH) andC-reagent as raw materials, and basic zinc carbonate (P_Z_n) and rubber solvent oil (120Gasoline) as pore-forming material. Compared with the technique of the amorphous lumpresin and that of the resin synthesized in oil phase, the new technique of resin synthesizedin aqueous phase manifests many advantages, such as raising the capacity of uranium-adsorption of the resin, simplifying the after-treatment process, reducing the cost, etc. Sea water, in which all kinds of cation and anion exist, is a system of electrolytes,and the concentrations of many ions are higher than the concentration of uranium for     

A COMPARATIVE STUDY OF 1982-1983 EL NIÑO EVENT

The 1982-1983 El Niño event is examined in comparison with the other El Niño events in 1949-1983. The relationships of subtropical high to the sea surface temperature (SST) show the important influence of the atter, which vaired with the characters of El Niño events.     

头足类视觉特性的电生理研究 Ⅰ.无针乌贼视网膜电图的特性

乌贼是重要的海洋水产资源之一,对其视觉特性的研究在理论上和实践上显然都有一定的意义。视网膜电图(ERG)作为网膜的综合电活动是反映视觉特性的一个良好指标。乌贼的ERG的特性至今缺乏系统的研究。本文对其ERG的波形和适应特性作了较详细的描述。续文将报导对ERG的光谱敏感性的分析。     

网格剖分积分法计算富钴结壳资源量

在矿区地质模型建立及边界条件限定的基础上,提出了基于网格剖分积分计算富钴结壳资源量的评估计算方法,推导了相应的计算公式,设计了相应的模块界面,并在"海底地形地貌自动成图系统"中实现了这种计算方法.这种资源量计算方法可应用于富钴结壳资源研究和评价计算.     

Bulk particulate organic carbon in the East China Sea: Tidal influence and bottom transport

Five oceanographic surveys were carried out in the East China Sea (ECS) and Yellow Sea from 1999 to 2003. In all, seven different sections were surveyed, but one section (the PN section) was observed on every cruise. Two time-series stations were also surveyed, one located at the Changjiang River mouth, the other over the continental shelf in the PN section.We identified biogeochemical characteristics for waters close to the Changjiang Estuary and in the Kuroshio waters (KW), respectively. Resuspension is a strong feature near bottom over the ECS continental shelf, with suspended matter values 13 times higher than that for the surface. A model of particulate organic carbon (POC) dynamics based on a rectangle equation reveals that POC concentration close to the Changjiang Estuary varies with a semidiurnal period of ∼13 h, coinciding with the tidal period. The upper limit for POC residence times in the seasons we covered over the shelf are estimated to be on the order of weeks and generally increase seaward from near the Changjiang Estuary to the KW. Short POC residence times suggest that POC in the ECS is rapidly exported from euphotic waters.A nepheloid layer, observed as elevated suspended matter in near the bottom of the water column, is important in particle transfer over the shelf, especially in winter when the residual current flows mainly eastward. Cross-shelf transport of POC via the nepheloid layer is estimated to be 0.22 × 10¹² g yr⁻¹. Comparison with other work indicates that POC transport is ∼2% of the Changjiang POC input.     

具有内波特性的渤海南部次重力波

次重力波(infragravity waves)是频率范围介于局地平均Brunt-V¾is¾l¾频率和0.05Hz(涌浪频率)之间的一种海洋波动,其能量在深水大洋中一般甚低,但在浅海中却很强.该波动的研究对于浅海海洋内部混合、近岸海洋工程、海洋生态学、海洋沉积学及军事海洋学均有重要的意义.Munk[1]很早就开始研究这类波动,然而主要由于观测技术的限制,对于该类波动的动力学机制的研究进展迟缓.     

斑鳢(Channa maculata)微囊藻毒素去毒相关基因sGST的克隆与序列分析

采用RT-PCR技术和RACE技术成功克隆淡水鱼类斑鳢sGST基因cDNA全序列,推测得到氨基酸序列,初步分析其结构功能域及系统进化关系。结果表明,斑鳢sGST基因cDNA序列全长为898bp,编码225个氨基酸。斑鳢sGST与真鲷、金头鲷、鲽、黑头鲦、川鲽、大口黑鲈等最新定名为ρ型的sGST氨基酸同源性较高,ρ型sGST为水生生物所特有并共同占据进化树上独立的分枝;与大鼠、小鼠、人等哺乳动物sGST现有所有类型同源性均很低,并且在进化树上距离也较远,表明本研究成功克隆的sGST基因应属于ρ型,可能在鱼类等水生生物对水栖环境的适应上有重要作用。     

超声波对湛江等鞭金藻生长和脂肪酸组成的影响

设计了一个超声频率、超声功率和处理时间三因素四水平的正交实验,研究了超声波对湛江等鞭金藻的生物学效应.实验结果表明,超声频率对湛江等鞭金藻的生物学效应显著,而超声功率和处理时间的影响相对较小.对于提高湛江等鞭金藻生长速率常数,最佳超声条件为20kHz,6W,10s3次;对于提高其脂肪酸不饱和度和主要不饱和脂肪酸百分比,最佳超声条件为20kHz,4～6W,30s.在最佳超声条件下,湛江等鞭金藻生长速率常数最高可达0.630d,是对照组的2.02倍,其脂肪酸不饱和度最高可达79.6%,比对照组提高7.8%,其中主要不饱和脂肪酸的百分含量均得到不同程度的提高.     

海水中金属设施防腐、防海洋生物污损及检测技术研究

随着工业的高速发展和人口的急剧増加及环境污染的加剧，淡水资源危机已引起世界各国的普遍关注。据统计，城市用水中工业用水通常占80%左右，而工业用水中有80%是工业冷却用水，所以开发利用海水代替淡水直接作冷却用水是节约淡水的重要途径。海水直流冷却系统具有深海取水温度低且恒定、冷却效果好和系统管理简单等优点，但必须具备以下条件:(1)管道系统防除生物附着污损；(2)管道系统的防结垢；(3)金属腐蚀与防护。多年来有关方面虽然相应地采取了许多措施，但由于主设备通常需常年连续运行，因而往往出现在某段时间、某些部位上效果降低与失效。现行的对海水环境中生物附着腐蚀检测主要采取取样离位分析方法，其结果与实际状态有差异，且生过程中许多重要部位，如海上平台、浮码头、船舶、海滨电厂、化工厂的海水冷却系统和海水管路的管道内、及死角区都很难取样，必要时只有定期停机检查(侯保荣1998； Efird，1976；吉井彻，1967)，造成很大的经济损失。 防止海水环境中的金属设施遭受海水腐蚀和海洋生物污损的方法很多，通常采用阴极保护、防污涂料、电解海水或电解重金属等方法，但这些方法的缺点是：(1)阴极保护能防止金属腐蚀而不能防止海洋生物附着；(2)防污涂料涂刷一次，只能用3-5年，对许多一次性埋入水中的管件无法进行二次补涂，防污涂料所释放出的毒物将长期污染环境；(3)利用电解海水或电解重金属所产生的次氯酸钠或重金属离子来进行防污，即电解防污效果明显，但对金属防腐的作用不大(侯保荣，1999;陈光章，1994;马土德，1996)。总结前人的经验，经过多年的研究，我们利用电化学方法研制出既能防止金属海水腐蚀又能防生物附着的双防与检测新技术系统装置，已通过实海实验，取得了良好的效果。