青岛文昌鱼肌球蛋白重链基因片段的重组表达

将青岛文昌鱼(Branchiostoma belcheri tsingtauense)肌球蛋白重链基因片段亚克隆到pET-30a质粒,构建出重组表达载体并转化入大肠杆菌Escherichia coli BL21中.经SDS-PAGE和Western杂交检测表明,在IPTG诱导下含有重组载体的菌株可表达分子质量约30 ku的融合蛋白.诱导条件优化实验结果显示,该菌株经0.2 mmol/L IPTG诱导1 h就可大量表达此蛋白.可溶性实验确认该重组蛋白是可溶性蛋白.本研究为文昌鱼肌球蛋白重链特异性抗体的制备奠定了基础.     

福建漳江口红树林保护区浮游植物群落的季节变化研究

于2001年1月～2003年1月对福建省漳江口红树林区水体浮游植物群落的季节变化进行了研究。结果表明,漳江口红树林区浮游植物以硅藻门种类为主,优势种为长菱形藻(Nitzschia longissima)和菱形藻(Nitzschia sp.1)等,同时出现多种裸藻、绿藻和甲藻。本次调查共鉴定到浮游植物31属87种(包括变种),其中硅藻门23属75种(包括变种),蓝藻门3属3种,绿藻门1属4种,金藻门1属1种,甲藻门2属2种,裸藻门1属2种。浮游植物密度变化范围为2.78×104～1.14×106个/L,平均为3.51×105个/L,季节变化为双峰型。出现大量的底栖硅藻和淡水性藻类是该水域浮游植物的一个特点。浮游植物的组成和结构表明该水域水质较好。     

中国海监船舶导航与监控系统的研究

海监船舶在维护南海海洋权益、实施海域使用管理、海洋环境保护和海洋防灾救灾等方面起着重要的作用。文章从技术实现角度出发,结合海监船舶的航行方法、区域及受控监管特点,对海监船舶导航及监控的技术和信息需求做了深入研究,设计并基本实现了“海监船舶导航与监控管理信息系统”。     

红树林单宁的研究进展

植物单宁(vegetable tannin)，又称植物多酚(plant polyphenol)是一类广泛存在于植物体内的多元酚化合物，在维管植物中的含量仅次于纤维素、半纤维素和木质素，主要存在于植物的皮、根、叶、果中，含量可达20％。作为皮革的一种传统鞣剂，单宁一般指的是相对分子质量为500～3000的多酚。Haslam提出了植物多酚这一术语，它包括了单宁及相关化合物(如单宁的前体化合物和单宁的聚合物)。根据化学结构的不同，     

中太平洋西部深海沉积物中的火山碎屑与粘土矿物

对于太平洋深海沉积物的研究虽有很久的历史(如1873-1876年“挑战者”号的科学考察),但对该区开展深入系统的岩石学和矿物学研究却是六十年代以后的事.随着研究手段的发展和进步,已积累了关于该区深海沉积物的大量资料.目前我国的海洋工作者也正在努力开展这方面的科学研究工作.     

Steady-state analysis of undersea cables

A numerical solution and computer program developed to analyse undersea flexible cable problems is presented. The cable is subject to hydrodynamic loads from a surrounding fluid medium. The object is to determine the cable profile and tensile forces developed. The cable equations and boundary conditions result in a classical two-point boundary value problem. An iteration ‘shooting method’ is used for solution. The program has been written to analyse a class of towing and anchoring problems. However the analysis is general and applicable to most steady-state cable problems.     

栉孔扇贝(Chlamys farreri) 血细胞类型及抗菌力的研究

用2种方法将栉孔扇贝血细胞分为2大类透明细胞、颗粒细胞.吉姆萨染色观察,透明细胞的细胞质中颗粒很少,细胞核圆形,居中,细胞质较少,核质比大;颗粒细胞比透明细胞大且多,染色深,但核质比小,在细胞质中有颗粒,有些颗粒细胞具双核.密度梯度离心结果,透明细胞主要位于上层,颗粒细胞则大部分位于下层.吞噬力、抗菌活力、溶菌活力及活性氧检测结果表明,颗粒细胞均强于透明细胞.     

智能潜器的集成仿真系统

本文介绍了智能潜器集成仿真系统的硬、软件结构．通过这一系统所展示的水下虚拟仿真环境能够在研究和开发智能潜器的控制体系结构、潜器载体的水动力学、信息融合和目标识别等工作中发挥巨大作用     

南海尖峰海山多金属结壳地球化学

南海尖峰海山多金属结壳富含30多种元素,其锰铁矿物主要由钡镁锰矿,δ-MnO_2和FeOOH·xH_2O组成。与其它海区的结壳相比,尖峰海山结壳富含Cu、Ni、Ba、Zn、Pb等元素,而Co、Ti、稀土元素(REE)、Sr等元素相对较贫。研究表明,HREE亏损,具明显的Ce正异常,较明显的Tb正异常和Yb负异常。这是氧化弱碱性海洋环境所致。结壳是水成作用的产物,它的形成受南海独特的古海洋环境所控制,海底火山热液作用,可能也是影响因素之一。     

A nitrate and silicate budget in the equatorial Pacific Ocean: a coupled physical–biological model study

A coupled physical–biological model was developed to simulate the low-silicate, high-nitrate, and low-chlorophyll (LSHNLC) conditions in the equatorial Pacific Ocean and used to compute a detailed budget in the Wyrtki box (5°N–5°S, 180–90°W) for the major sources and cycling of nitrogen and silicon in the equatorial Pacific. With the incorporation of biogenic silicon dissolution, NH₄ regeneration from organic nitrogen and nitrification of ammonia in the model, we show that silicon recycling in the upper ocean is less efficient than nitrogen. As the major source of nutrients to the equatorial Pacific, the Equatorial Undercurrent provides slightly less Si(OH)₄ than NO₃ to the upwelling zone, which is defined as 2.5°N–2.5°S. As a result, the equatorial upwelling supplies less Si(OH)₄ than NO₃ into the euphotic zone in the Wyrtki box, having a Si/N supply ratio of about 0.85 (2.5 vs. 2.96 mmolm⁻² day⁻¹). More Si(OH)₄ than NO₃ is taken up with a Si/N ratio of 1.17 (2.72 vs. 2.33 mmolm⁻² day⁻¹) within the euphotic zone. The difference between upwelling supply and biological uptake is balanced by nutrient regeneration and horizontal advection. Excluding regeneration, the net silicate and nitrate uptakes are nearly equal (1.76 vs. 1.84 mmolm⁻² day⁻¹). However, biogenic silica export production is slightly higher than organic nitrogen (1.74 vs. 1.59 mmolm⁻² day⁻¹) following a 1.1 Si/N ratio. In the central equatorial Pacific, low silicate concentrations limit diatom growth; therefore non-diatom new production accounts for most of the new production. Higher silicate supply in the east maintains elevated diatom growth rates and new production associated with diatoms dominate upwelling zone. In contrast, the new production associated with small phytoplankton is nearly constant or decreases eastward along the equator. The total new production has a higher rate in the east than in the west, following the pattern of surface silicate. This suggests that silicate regulates the diatom production, total new production, and thereby carbon cycle in this area. The modeled mean primary production is 48.4 mmolCm⁻² day⁻¹, representing the lower end of direct field measurements, while new production is 15.0 mmolCm⁻² day⁻¹, which compares well with previous estimates.