Development of a real-time PCR method for Thalassiosira rotula rapid detection

Gene specific primers and DNA probe were designed based on the sequence of 18S rDNA cloned from the red tide alga Thalassiosira rotula. A real-time fluorescent quantitative PCR (RFQ-PCR) method was developed for quantitative detection of T.rotula. The RFQ-PCR assay data showed that the results obtained with the RFQ-PCR quite good agreement with those with the light microscope (LM) counting method, which suggested that the RFQ-PCR could be a useful method for red tide alga detection.     

几株原甲藻核糖体大亚基RNA基因的部分克隆及序列分析

对7株赤潮原甲藻28S rDNA 5'端部分序列进行扩增、克隆和序列测定,并从GeneBank上获取14个原甲藻28S rDNA序列,用NJ法和ME法构建了原甲藻属的系统树,并对序列进行分析.结果表明,7株原甲藻28S rDNA扩增序列长度为950~958 bp,通过NJ法和ME法构建的系统树完全一致.大部分分离自不同海域的同种原甲藻的序列高度保守,而不同种间在序列高变区却有较大的差异.但来自南海海域的海洋原甲藻(Prorocentrum micans)与分离自其他海域的株系序列差异较大,甚至超出了有些种间的差异.由28S rDNA高变区获得的序列,有望成为浮游植物特异性分子探针设计的良好靶区域.     

Calculating Pollution Indices by Heavy Metals in Ecological Geochemistry Assessment and a Case Study in Parks of Beijing

Pollution index is a powerful tool for ecological geochemistry assessment. The commonly used pollution indices by heavy metals in soils and sediments were classified as two types of single index and integrated index in an algorithm point of view. Four single indices of contamination factor (or concentration factor), ecological risk factor, enrichment factor, and index of geo-accumulation were illustrated, and the reference values for calculating single indices were distinguished into background levels and threshold pollution values. Eight integrated indices were divided into two groups. One group is suitable for the normal distribution single indices including the sum, average, weighted average,vector modulus, and Nemerow pollution indices, and the other for log-normal distribution including the product, root of product, and weighted power product pollution indices. Using background levels as reference values, five contamination classes were divided, and the terminologies are suggested for the single and integrated indices to unify the assessment results. Software of EGAPI was developed in a single document interface to calculate the four single and eight integrated indices by heavy metals to assess the quality of soil and sediment ecological geochemistry. Pollution indices by heavy metals of Cu,Pb, and Zn in soils in parks of Beijing were calculated using EGAPI software, and these five contamination classes and terminologies suggested in this study were evaluated and used. Results ofintegrated indices of Cu, Pb, and Zn in soils indicated that the soil qualities are unpolluted as a whole and varied from low polluted to unpolluted status from the center to the outskirts of Beijing City.     

基于LBV变换的遥感影像多步骤分类法研究

面对与日俱增的遥感数据,如何快速准确地进行影像分类成为遥感领域亟待解决的问题。本文在前人的基础上提出了一种基于LBV变换的遥感影像多步骤分类方法。L:地物的总辐射水平,集中反映了裸地的信息;B:地物的可见-红外光辐射平衡,是地面水分状况和水体存在的一个良好指标;V:地物辐射随波段变化的方向和速度向量,能集中反映地面植被状况。多步骤分类法遵循由易到难的原则,能克服类别之间的互相影响,从而提高分类精度。文章结合LBV变换和多步骤分类法的优点对影像进行了分类,结果表明该方法简单易行,且能达到良好的分类精度。     

基于ODBC的地震前兆数据批量卸出与转换程序的实现

介绍了CDBC技术、工作流程及数据传递方式；分析了研制数据卸出与转换程序的关键技术及实现方法；描述了程序的组织结构。通过实例说明了该程序在全国前兆台网中心卸载与转换地震前兆数字化的观测数据中的应用。     

青藏铁路抛石路基的温度特性研究

铁路道渣和片石铺层的对流换热为多孔介质的热传导问题，根据多孔介质中流体热对流的连续性方程、动量方程和能量方程，应用伽辽金法导出了多孔介质对流换热的有限元公式，并对抛石路基和传统道渣路基在未来25a创温度变化进行了预报分析和比较．计算结果表明，在150cm的抛石厚度，片石直径为10cm，年温度较差30℃的倩况下，在路基中心线y＝一5m处，抛石路基下的冻土温度要比传统路基的温度低2．45℃，抛石路基有对其下面的冻土提供冷能的制冷作用，可以保证冻土路基的稳定．因此，推荐该种路基作为青藏铁路高温冻土区的路基结构，可以最大限度地保护冻土区的铁路．     

开发利用地理信息系统(GIS)综合分析地学信息进行矿产预测

众所周知,矿产资源预测是综合地学信息,进行优选靶区的有效手段之一。随着地学工作的深入和勘探技术的发展,已获取了大量的多源地学信息,如地质、地球物理、地球化学和遥感等资料。怎样从这众多的资料中提取有用信息进行综合分析,达到矿产资源预测的目的,一直是地学界探索的课题。过去,应用人工的方法来进行此项工作,不但费力、投资大,而且难以达到预期的效果,方法技术也不利于推广。如今,高速、高质量的计算机已趋于普及,使应用计算机技术高效地处理堆积如山的资料成为可能,尤其是近些年发展起来的地理信息系统(GIS),为综合处理地学资料的矿产资源预测的方法技术,开拓了广阔的前景。地理信息系统是一种计算机系统的应用软件,它集数据库管理和图像分析技术为一体,以空间数据迭加分析为重要特征。地学工作者进行矿产资源预测时,通常是从多源地学资料中提取有用信息,再应用专家知识,结合数学方法,如逻辑运算、贝叶斯(Bayes)规则等,建立预测模型,以模型的推理网格为线索,利用地理信息系统有效的空间分析手段,把各种证据图层综合迭加,最终产生以概率为指标的矿产资源预测图,高概率指示有利的矿产资源远景区,可作为勘探者和决策者进行勘查规划的依据。     

鄂尔多斯盆地奥陶系海相碳酸盐岩生烃性能与中部长庆气田气源成因研究

用激光 -荧光显微镜等有机岩石学方法 ,挑选样品中有明显生、排烃现象的碳酸盐岩 ,再以测定的这类碳酸盐岩的有机碳含量为依据 ,确定本区高成熟碳酸盐气源岩残余有机碳的下限指标用 0 .13%～ 0 .14 %比较合理。根据碳酸盐岩储层孔隙中充填沥青和皮膜状残余沥青的分布和含量 ,论证了陕参 1井等奥陶系碳酸盐岩地层在地质历史中 ,存在若干油气有效储层。采用连续升温 ,步进取样的热模拟实验方法 ,揭示了本区煤与碳酸盐岩干酪根随热演化作用增强 ,烃类气体产物的干燥系数增大 ,δ1 3C1 、δ1 3C2 明显变重的规律 ,并根据样品实验结果推算 ,本区石炭系—二叠系煤最大生气阶段的δ1 3C1 应为 - 2 7‰～ - 2 8‰ ,碳酸盐岩最大生气阶段的δ1 3C1 应为- 31.7‰～ - 33‰。在气源成因判识中 ,根据主生气期阶段对天然气成藏储聚的贡献最大、甲烷碳同位素较重以及长庆气田风化壳气藏天然气δ1 3C1 重值区并不与石炭系—二叠系煤的镜质组反射率高值区匹配的现象等进行气源判识 ,认为长庆奥陶系风化壳气藏 ,具有下古生界海相碳酸盐岩气与上古生界石炭系—二叠系煤成气的混源成因     

Determination of paleo-pressure for a natural gas pool formation based on PVT characteristics of fluid inclusions in reservoir rocks——A case study of Upper-Paleozoic deep basin gas trap of the Ordos Basin

~~Determination of paleo-pressure for a natural gas pool formation based on PVT characteristics of fluid inclusions in reservoir rocks——A case study of Upper-Paleozoic deep basin gas trap of the Ordos Basin~~     

雅安芦山4.20震后县级单元生态系统风险评价

区域生态风险评价是对各种生态风险及环境问题进行评价和管理的重要手段。针对雅安地震灾区特殊的自然地理及生态环境特征,选取芦山县为研究对象,采用遥感、GIS及SPSS统计分析的方法,通过风险源、风险受体、暴露和易损性分析,建立生态风险综合评价模型,划分生态风险区类型,进而提出生态风险管理对策。结果表明:1)微度和低度生态风险区集中分布在高海拔的森林及草地生态系统,该区生物多样性丰富,抗干扰能力较强,地质灾害及人类活动影响较小;2)中度和高度生态风险区具有沿农田及建设用地生态系统集聚分布的特征,该区地质灾害频繁,地壳活动性较强,生态系统抵抗灾害的能力较差。研究结果可为地震灾区防御、规避风险及安全选址提供科学依据。