Molecular Characterization and Expression Analysis of SKIV Infection of Interferon-Induced Protein with Tetratricopeptide Repeats 1(IFIT1) in Epinephelus lanceolatus

Interferon-induced protein with tetratricopeptide repeats 1(IFIT1), also known as interferon-induced protein 56(IFI56) or Interferon-stimulated protein 56(ISG56), was originally identified as a protein induced upon treatment with interferon and inhibited by viral replication and translational initiation. In this study, Epinephelus lanceolatus IFIT1(ELIFIT1) gene was cloned for the first time. The complete cDNA of El IFIT1 gene includes 2921 nucleotides, and encodes a 437-amino acid(AA) protein. The putative ELIFIT1 protein has 9 TRP domains and is highly similar with IFIT1 proteins in other teleosts. In healthy fish, ELIFIT1 gene was highly expressed in the blood, which indicate its specific function in the peripheral immune system. Its expression was also observed in various immunity-related tissues including spleen, intestine, and kidney, Inducted with spotted knifejaw iridovirus(SKIV), ELIFIT1 gene expression was upregulated in the spleen, kidney, and liver 24 h after induction and reached its peak at 72 h, indicating that ELIFIT1 may play an important role in antivirus. These findings contribute to the understanding of the antiviral regulation of ELIFIT1 gene in teleost.     

提高鳜鱼催产率受精率和苗种成活率的试验

在常规催产剂中加入适量的Ａ型添加剂，可使鳜鱼的催产率提高１０％以上，受精率提高５％以上。使用１５×１０－６的福尔马林，可有效地杀灭寄生于鳃及体表的寄生虫和细菌，从而大大提高苗种成活率。     

Advances in studies of the effects of starvation on growth and development of fish larvae

Starvation has important effects on early development of fish. It determines the survival and growth of fish larvae, and plays an important role in the dynamics of fish population and fisheries recruitment, in this review, we discuss the current studies about the effects of starvation on growth and development of fish larval stage. The goals of this review are to understand some adap- tive mechanisms and ecological countermeasures of starved fish larvae and to provide the scientific guideline for exploring early life history processes, evaluating the nutrition condition and growth of larval fish, protecting fish resource and breeding fish larvae.     

Hydroacoustic estimates of fish biomass and spatial distributions in shallow lakes

We conducted acoustical surveys with a horizontal beam transducer to detect fish and with a vertical beam transducer to detect depth and macrophytes in two typical shallow lakes along the middle and lower reaches of the Changjiang(Yangtze) River in November 2013. Both lakes are subject to active fish management with annual stocking and removal of large fish. The purpose of the study was to compare hydroacoustic horizontal beam estimates with fish landings. The preliminary results show that the fish distribution patterns dif fered in the two lakes and were af fected by water depth and macrophyte coverage. The hydroacoustically estimated fish biomass matched the commercial catch very well in Niushan Lake, but it was two times higher in Kuilei Lake. However, acoustic estimates included all fish, whereas the catch included only fish 45 cm(smaller ones were released). We were unable to determine the proper regression between acoustic target strength and fish length for the dominant fish species in the two lakes.     

一株溶藻弧菌噬菌体的生理特性研究

对从湛江市水产品批发市场分离得到一株溶藻弧菌噬菌体的生理特性进行测定,结果表明:该株噬菌体噬菌斑12 h直径为1～1.5 mm,对紫外线敏感,暴露在紫外灯(20 W,30 cm)下30 min可丧失全部裂解活性;在pH8～11范围内裂解活性较强,最适pH值为11,裂解周期为65 min,对65℃以上的高温敏感,对乙醚和氯仿有较好的耐受性,对抗病毒药物阿昔洛韦敏感,最大常规稀释度为106CFU/mL,最佳感染复数为10。     

镇江长江豚类省级自然保护区鱼类多样性及年际变化

【目的】调查了解镇江豚类保护区鱼类资源状况,丰富鱼类资源本底数据。【方法】于2013、2016和2019年,分别开展3次渔业资源调查,对该区域鱼类种类组成、优势种、体型大小及群落多样性进行统计分析。【结果】共记录鱼类68种属于7目13科47属;鲤形目(Cypriniformes)鱼类种类占比较为稳定,鲇形目(Siluriformes)鱼类资源量有下降趋势,鲈形目(Siluriformes)鱼类种类占比上升明显;不同年份优势种替代较为明显;2016年的物种丰富度指数和多样性指数最高,分别为6.528、3.026。【结论】保护区鱼类资源较为丰富,但不同年份鱼类群落变化较为明显,渔业资源面临小型化趋势。     

Significant effects of fishing gear selectivity on fish life history

Over the past few decades, extreme changes have occurred in the characters of exploited fish populations. The majority of these changes have affected the growth traits of fish life history, which include a smaller size-at-age, an earlier age-at-maturation and among others. Currently, the causes of these life history traits changes still require systematic analyses and empirical studies. The explanations that have been cited are merely expressed in terms of fish phenotypic adaptation. It has been claimed that the original traits of fish can be recovered once the intensity of exploitation of the fish is controlled. Sustained environmental and fishing pressure will change the life history traits of most fish species, so the fish individual’s traits are still in small size-at-age and at earlier age-at-maturation in exploited fish populations. In this paper, we expressed our view of points that fishing gear has imposed selectiv-ity on fish populations and individuals as various other environmental factors have done and such changes are unrecoverable. Ac-cording to the existing tend of exploited fish individual’s life history traits, we suggested further researches in this field and provided better methods of fishery management and thereby fishery resources protection than those available early.     

Potential hazards in smoke-flavored fish

Smoking is widely used in fish processing for the color and flavor. Smoke flavorings have evolved as a successful alter- native to traditional smoking. The hazards of the fish products treated by liquid-smoking process are discussed in this review. The smoke flavoring is one important ingredient in the smoke-flavored fish. This paper gives the definition of smoke flavorings and the hazard of polycyclic aromatic hydrocarbons (PAHs) residue in the smoke flavorings on the market. It gives also an assessment of chemical hazards such as carcinogenic PAHs, especially Benzo-[a]pyrene, as well as biological hazards such as Listeria monocyto- genes, Clostridium botulinum, histamine and parasites in smoke-flavored fish. The limitations in regulations or standards are dis- cussed. Smoke flavored fish have lower content of PAHs as compared with the traditional smoking techniques if the PAHs residue in smoke flavorings is controlled by regulations or standards.     

广西桂平东塔河段产卵场水质综合评价

为了保护渔业生态环境和渔业资源,从2001-2003年对广西桂平东塔鱼类产卵场水体实施了连续调查监测,对该水域水质的时空变化趋势和质量状况进行了研究和综合评价。结果表明,东塔产卵场水体处于相对清洁至轻污染状态;总氮、总磷、锌是重点监测和控制的优先污染物。     

Structure and Dynamics of Fisheries in Nigeria

The changes that have taken place in Nigerian fisheries are reviewed. Artisanal fishery has continued to dominate the fisheries,contributing over 85% of total fish production. The inland water and coastal seas are fully exploited and the increase in fishery production is not likely. Aquaculture potentials remain untapped as much as deep-sea fisheries. The combined potential of the fisheries resources-freshwater,marine and aquaculture can meet over 90% of the nation's demand for fish. Opportunities for invest-ments,therefore,exist in the various subsectors,especially in the areas of storage,processing and preservation for the capture fishery and fish seed multiplication for aquaculture.     

Larva fish assemblage structure in three-dimensional floating wetlands and non-floating wetlands in the Changjiang River estuary

Fish populations have declined in many estuarine and freshwater ecosystems in part due to the loss of habitat in recent decades.Reconstructing lost habitat for larvae fish is a potential method for recovering larvae fish populations.Three-dimensional artificial floating wetlands(AFWs) on which Phragmites australis was planted were experimentally deployed to recover the lost habitat in the Changjiang(Yangtze) River estuary from May to July 2018.The AFW area was characterized by slow velocity,high transparency,low dissolved oxygen,and relatively constant water temperature.The total individuals of larvae fish in the AFW area(12 122 in total) was higher than that in the non-AFW area(1 250 in total),and the densities of most larvae fish species were higher in the AFW habitat than in the non-AFW area.The distributions of larvae fish species were positively influenced by habitat type because they were strongly related to the negative part of the first axis of the redundancy analysis,and Cyprinus carpio and Cyprinus auratus were inclined to habitat in the slow velocity and high transparency AFW habitat area.These results indicate that larvae fish species are inclined to inhabit the AFW habitat.The use of three-dimensional P.australis AFWs would be a potential method for enhancing the habitat of larvae fish in the degraded habitats along the estuary.     

雷州半岛红树林海区的鱼类种类调查

对雷州半岛8个红树林分布区设站位进行了生物多样性调查,其中获得鱼类127种,分别隶属于15目58科100属。以鲈形目最多,共27科49属65种,占51.2％。种类出现最多的科为(鱼叚)虎鱼科。采获种类最多的站位为北潭。各站位之间鱼类种类具有一定的差异性。调查获得的鱼类体型偏小,大部分种类具有食用价值,其中16种已为人工增养殖的对象。     

金枪鱼油的精炼工艺对脂肪酸组成的影响

粗鱼油通过精炼可以除去非甘油三酯杂质成分,研究表明黄鳍金枪鱼鱼油精炼的工艺参数为:800 g/L的H3PO4脱胶,添加量为油量的1%(体积分数);300 g/L的NaOH脱酸,添加量为油量的2%(体积分数);活性土脱色,添加量为油量的10%(质量分数);85℃下减压蒸馏脱臭15 min。经过精炼,鱼油呈淡黄色,澄清透明,有淡鱼腥味,理化指标达到鱼油SC/T3502-2000标准的精制鱼油一级要求,精制鱼油中PUFA的总含量(w)达37.06%,DHA和EPA含量(w)分别为25.10%和4.45%。     

一种改进的人工鱼群聚类算法

为了克服K-Means算法对初始类簇中心、噪声点、孤立点敏感缺点,将K-Means算法和人工鱼群算法结合,提出了改进的人工鱼群聚类算法。在该算法中将类簇中心看作一条人工鱼,让每条人工鱼执行随机、觅食、聚群、追尾行为中的一种,并将更新后的位置作为K-Means算法的初始值,不断重复人工鱼的位置更新和K-Means操作,直到算法结束。由于在算法中加入了动态移动步长和全局人最优人工鱼位置,聚类的收敛精度和速度都得到提高。使用iris和glass数据集进行聚类时,与其他算法相比,文中的收敛时间缩短2.6%,精度提高1.36%。     

Experimental Study on the Hydrodynamic Characteristics of a Submersible Fish Cage at Various Depths in Waves

Submersible fish cages can be submerged under the water to mitigate the negative effects that arise from severe sea conditions and improve the growing environment for the farmed fish. Thus they are increasingly applied in offshore aquaculture. To ensure both safety and economic efficiency of submersible fish cages, it is important to determine the optimum submergence depth. In this study, a series of physical model experiments were conducted to investigate the hydrodynamic performance of a submersible fish cage at various submergence depths(1/6, 1/4, 1/3, and 1/2 of the water depth as well as the floating condition for reference) with a model scale of 1:20. The results of the physical model experiment for the different depths were compared to analyze the effects of submergence depths on the mooring line tension and the movement of the floating collar. The results showed that the mooring line tension and the floating collar movement significantly attenuated with increasing submergence depth. However, the attenuation tendency became stable when the fish cage reached a certain depth. According to the results, 1/3 of water depth was determined as the optimal submergence depth of the fish cages. Deeper submergence depths showed no significant advantage from a perspective of the hydrodynamic characteristics of the fish cage. The determination of the optimum submergence depth is beneficial for the structural design and operation safety of submersible net cages.     

Clean and safe supply of fish and shellfish to clear the HACCP regulation by use of clean and cold deep ocean water in Rausu, Hokkaido, Japan

For the supply of fish and shellfish to consumers in fresh condition, clean handling after catch from the sea is essential. According to HACCP （Hazard Analysis and Critical Control Points）, it is important to meet such requirement by keeping fish and shellfish under a certain low temperature and clean conditions after catching. The deep ocean water （DOW） characterized by low temperature and cleanliness has been chosen for fish and shellfish handlings, particularly for salmon, cod, and sea urchin in Town ＇Rausu＇ in Hokkaido, Japan. DOW below 2.9℃ of an amount of nearly 5 000 m^3 is planned to be pumped up every day from a depth of about 350 m, and temporarily stored in a large simulated tank on land. DOW is then supplied to fish boats through hydrants distributed throughout the harbor and used for keeping salmon in clean and cold conditions. Ice made from DOW is also used for lowering temperature if necessary. DOW and ice made from DOW are also used during the transportation of fish and shellfish. The entire system is scheduled to be completed by the summer of 2005.     

Microbiota and Shelf Life of Whole and Gutted Pacific Saury(Cololabis saira) During Refrigerated Storage

Pacific saury(Cololabis saira)is usually sold as whole fish in wholesale markets,or in its gutted form which is easy for consumers to cook in retail markets.In order to assess the effect of gutting on the shelf life of Pacific saury during refrigerated storage and reveal the microbial community,fish samples grouped into(I)whole fish and(II)gutted fish were analyzed periodically for sensory,biochemical and microbiological characteristics,and high-throughput sequencing technology was used to investigate the microbiota.Results showed that the sensory score for the gutted fish became unacceptable on day 8,while the whole fish score remained acceptable for 10 days.The total volatile basic nitrogen(TVB-N)value of the gutted fish reached 30 mg N(100 g)^-1 on day 6,while that of the whole samples surpassed 30 mg N(100 g)^-1 on day 10.The thiobarbituric acid reactive substances(TBARS)value of the gutted fish got close to 5.0 mg kg^-1 on day 10,while that of the whole samples surpassed 5.0 mg kg^-1 on day 14.The aerobic plate colony counts(APCs)for the gutted and whole Pacific saury reached 7.0 log10 CFUg^-1 on days 6 and 10,respectively.Organoleptic,biochemical and microbiological analyses revealed that shelf-lives should be 6–8 days for gutted fish and 10 days for whole fish.Microbiota analysis results showed that gutting partly changed the initial microbiota,but didn't alter the dominant bacteria during storage.When the fish were spoiled,high proportion of Pseudomonadaceae was detected in both groups.     

Are the fish of the upper and lower Mekong interconnected？

The Mekong supports one of the richest inland fisheries in the world, with many of the fish migrating long distance to spawn. Little is known about the fisheries and migration strategies of the Upper Mekong whilst it is supposed that many fish species move between the Lower and Upper Mekong. Most likely, natural fish migration in the river has been altered by dam construction across the mainstream of the Upper Mekong. In this paper, the interconnectivity of fish species between different sections of the Mekong and negative impacts of dams on migratory fish are studied. Of the 162 fish species in the Upper Mekong and the 869 species in the Lower Mekong, 61 species are common. Results show that there is no significant difference at order level between the UM and LM. Similarity coefficients are used to evaluate interconnectivity at species, genus and family levels among four different sections of the Upper Mekong with each other and with the Lower Mekong as a whole. The highest similarity is found between the middle and lower reach of the Upper Mekong at species and genus levels and the middle and upper reach at family level. Of the eight cascade dams, Mengsong Dam in planning is considered as the biggest threat to migratory fish from the Lower Mekong and should be particularly concerned. Supported by National Natural Science Foundation of China (No. 40601096)     

Viral Diseases of Olive Flounder in Korean Hatcheries

In order to elucidate the state of diseases, especially viral diseases, and to prevent viral diseases from occurring in olive flounder hatcheries, a range of studies, including epidemiological study, were performed from 1997 to 2003. The location of the hatcheries investigated includes several representative sites in the east （Kangnung, Uljin, Pohang, Yangsan, Ulsan, Pusan）, south （Wando, Changheung, Goheung, Yeosu, Namhae, Tongyeong, Geoje, Jeju） and west （Seosan, Kunsan, Gochang, Yeongkwang, Mokpo, Chindo） costal areas of the Korea Peninsula. A total of 2000 cases have been examined in 7 years, in which mortality caused by viral agents accounts for 22%, or 446 cases. Mortalities associated with viral infection considerably increased from 14% in 1997 to 27% in 2003. A variety of viral diseases were observed, and the occurrences of viral epidermal hyperplasia, viral ascites and viral deformity, viral nervous necrosis, and hirame rhabdoviral disease are 14%, 51%, 25%, and 8% respectively. By investigating the viral infection of broodstock flounder, the infection rate of marine birnavirus （MABV） in hatcheries was identified to be approximately 30%, therefore, it is highly necessary to acquire and keep non-infected broodstock fishes.     

Studies on Antiviral and Immuno-Regulation Activity of Low Molecular Weight Fucoidan from Laminaria japonica

The antiviral activity in vitro and in vivo and the effect of the immune system of two fucoidan fractions with low molecular weight and different sulfate content from Laminaria japonica(LMW fucoidans) were investigated in order to examine the possible mechanism. In vitro, I-type influenza virus, adenovirus and Parainfluenza virus I were used to infect Hep-2, Hela and MDCK cells, respectively. And 50% tissue culture infective dose was calculated to detect the antiviral activity of two LMW fucoidans. The results indicated that compared with the control group, 2 kinds of LMW fucoidans had remarkable antiviral activity in vitro in middle and high doses, while at low doses, the antiviral activity of 2 kinds of LMW fucoidans was not statistically different from that in the blank control group. And there was no statistically difference between two LMW fucoidans in antiviral activity. In vivo, LMW fucoidans could prolong the survival time of virus-infected mice, and could improve the lung index of virus-infected mice significantly, which have statistical differences with the control group significantly(p 0.01). However, the survival time of the two LMW fucoidans was not statistically significant(p 0.05). In this study, it was shown that both of two LMW fucoidans(LF1, LF2) could increase the thymus index, spleen index, phagocytic index, phagocytosis coefficient and half hemolysin value in middle and high doses, which suggested that LMW fucoidans could play an antiviral role by improving the quality of immune organs, improving immune cell phagocytosis and humoral immunity.