| 一株溶藻弧菌噬菌体的生理特性研究 |
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| 引用本文： | 曾林,邱德全,谢警鸿,于鸽.一株溶藻弧菌噬菌体的生理特性研究[J].广东海洋大学学报,2012(1):24-29. |
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| 作者姓名： | 曾林 邱德全 谢警鸿 于鸽 |
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| 作者单位： | 广东海洋大学水产学院;广东海洋大学广东省海洋开发研究中心 |
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| 基金项目： | 广东省科学技术厅项目(2009B020309002,2011B020307011);广东省海洋渔业局科技项目(A201008B05,A201008E03) |
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| 摘 要： | 对从湛江市水产品批发市场分离得到一株溶藻弧菌噬菌体的生理特性进行测定,结果表明:该株噬菌体噬菌斑12 h直径为1～1.5 mm,对紫外线敏感,暴露在紫外灯(20 W,30 cm)下30 min可丧失全部裂解活性;在pH8～11范围内裂解活性较强,最适pH值为11,裂解周期为65 min,对65℃以上的高温敏感,对乙醚和氯仿有较好的耐受性,对抗病毒药物阿昔洛韦敏感,最大常规稀释度为106CFU/mL,最佳感染复数为10。
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| 关 键 词： | 溶藻弧菌 噬菌体 生理特性 |
A Study of the Biochemical Characteristics of the Vibrio alginolyticus Bacteriophage |
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| ZENG Lin,QIU De-quan,XIE Jing-hong,YU Ge.A Study of the Biochemical Characteristics of the Vibrio alginolyticus Bacteriophage[J].Journal of Zhanjiang Ocean University,2012(1):24-29. |
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| Authors: | ZENG Lin QIU De-quan XIE Jing-hong YU Ge |
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| Institution: | 1(1.Fisheries College,Guangdong Ocean University,Zhanjiang 524025,China;2.Marine Development &Research Center of Guangdong Province,Guangdong Ocean University,Zhanjiang 524025,China;) |
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| Abstract: | The physiological characteristics of a Vibrio alginolyticus Bacteriophage was studied and the Bacteriophage was isolated from market of aquatic products in Zhanjiang.The results showed that the diameter of Bacteriophage plaque was 1-1.5mm(12h).Lytic activity of Bacteriophage lost within 30 min when Bacteriophage exposed to UV light(20W,30cm).Bacteriophage were UV-sensitive.They had strong lytic activity at pH 8-11,and the optimum pH value was 11.The lytic cycle of them was 65 min.They were sensitive as the ambient temperature was more than 65℃.They were tolerated with ether and chloroform.They were sensitive to antiviral drugs acyclovir.Routine test dilution(RTD) was 106.Optimal multiplicity of infection was 10. |
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| Keywords: | Vibrio alginolyticus Bacteriophage Biochemical characteristic |
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