南极酵母Rhodotorula sp.NJ298的鉴定及其产虾青素的研究

本研究对产虾青素的南极菌株NJ298进行了鉴定,同时对影响虾青素产量的主要环境因子进行了测试。形态特征、生理生化特征及ITS序列的系统发育分析表明,南极菌株NJ298属于红酵母属(Rhodotorula);虾青素积累主要在NJ298生长的对数期和稳定期;乙酸钠、蛋白胨是合成虾青素的最佳碳、氮源;在0-15℃之间,南极酵母NJ298均可以生长,但是低温更有利于虾青素的积累;pH值在6-8范围内NJ298均可生长,最适pH值为7.5;该菌对盐度要求不严格。     

产低温超氧化物歧化酶南极菌株的筛选、鉴定及其部分酶学性质

从421株南极细菌中筛选出产超氧化物歧化酶南极细菌NJ062、NJ379、NJ522和NJ548。它们的最适生长温度在10-15℃之间,在0℃均能生长,属于嗜冷菌。4株南极嗜冷菌均含有一种类型的SOD同工酶。菌株NJ062的超氧化物歧化酶为Mn-SOD;菌株NJ379,NJ522和NJ548的超氧化物歧化酶均为Fe-SOD。它们所产的超氧化物歧化酶最适酶活温度大约在40-50℃,在0℃仍有较高的活性,为最高活性的20%-35%,热稳定性较差,属低温酶。通过16S rDNA序列对4株产低温超氧化物歧化酶的南极嗜冷菌NJ062、NJ379、NJ522和NJ548进行鉴定。结果表明,它们均属于细菌域(Bacteria)中的紫细菌类群(Proteobacteria)中的γ-亚群。其中菌株NJ062属于假交替单胞菌属(Pseudoalteromonassp.);NJ379属于希瓦氏菌属(Shewanellasp.);NJ522和NJ548属于海单胞菌属(Marinomonassp.)。     

Screening and molecular classification of low-temperature protease from Antarctic microorganism and its characterization

<正> 107 strains producing protease were screened from 260 strains of Ant-arctic psychrophilic bacteria,among which proteolytic activity of five strains wasmore than 45 U ml~(-1).The 16S rRNA gene sequences homology and phylogenet-ic analysis of five Antarctic psychrophilic bacteria showed that NJ276、NJ5-9、NJ16-70、NJ345 belonged to the described genus Pseudoalteromonas and NJ341belonged to the genus Colwellia.The growth and the protease characteristic offour Antarctic psychrophilic bacteria had been studied,and the result showedthat the optimal temperature for growth and protease-producing of four strainswas about 10℃.Their growth and protease-producing were still high during in-cubating 2-5 days.The maximum proteolytic activity occurred at pH 9 for fourAntarctic psychrophilic bacteria.The optimal temperature of protease action ofboth strains NJ276 and NJ5-9 was about 50℃,however,the optimal temperatureof protease action of both strains NJ341 and NJ345 was about 40℃,and theirproteolytic activity under 0℃ exhibited nearly 30% of the maximum activity,but their thermal stabilities were weaker.These results indicated that proteasesfrom NJ341 and NJ345 were low-temperature proteases.     

老卤蒸发—冷却结晶脱镁的研究

采用蒸发—冷却结晶方法,对青海大柴旦盐湖提钾后的老卤进行脱镁试验研究。试验结果表明,一次蒸发温度为125℃时,老卤的镁去除率达到了33%,蒸发冷却后卤水的流动性较好。一次蒸发温度在135℃时,老卤的镁去除率达到了74%以上,但蒸发冷却后的卤水流动性差,过滤性能不佳。二次蒸发采用125℃蒸发后的卤水为原料,没有达到预期效果。     

2016年夏秋季南极布兰斯菲尔德海峡威氏棘冰鱼脂肪酸组成及其食性指示研究

作为20世纪70年代末期底拖网渔业的主要捕捞对象以及近年来南极磷虾渔业的兼捕对象之一，威氏棘冰鱼(Chaenodraco wilsoni)在海洋捕食者与饵料生物之间的能量流动中起着重要的传递作用，而目前针对该鱼种的营养特性及其食性的研究却十分有限。为此，本研究分析了布兰斯菲尔德海峡威氏棘冰鱼肌肉组织中脂肪酸的含量及其组成，并进一步探讨特征脂肪酸对食性的指示。结果表明，体长范围为4.8—30.7 cm、质量范围为0.3—250.5 g的威氏棘冰鱼肌肉样本中共检测出29种脂肪酸，其中含量较高的脂肪酸包括C16:0，C18:0，C18:1n9c，C20:5n3(EPA)以及C22:6n3(DHA)，各类脂肪酸类型含量由高至低依次为多不饱和脂肪酸(PUFA)，饱和脂肪酸(SFA)以及单不饱和脂肪酸(MUFA)。威氏棘冰鱼多种特征脂肪酸含量与体长之间不具显著的相关关系，表明其摄食并不随着个体的生长而发生较大的变化。秋冬季威氏棘冰鱼呈现杂食性特性，主要摄食以硅藻为食的生物，如南极磷虾(Euphausia superba); 同时对底栖生物和海底碎屑也有一定的摄食，并会摄食一定量的桡足类生物。)     

High-frequency rock temperature data from hyper-arid desert environments in the Atacama and the Antarctic Dry Valleys and implications for rock weathering

In desert environments with low water and salt contents, rapid thermal variations may be an important source of rock weathering. We have obtained temperature measurements of the surface of rocks in hyper-arid hot and cold desert environments at a rate of 1/s over several days. The values of temperature change over 1-second intervals were similar in hot and cold deserts despite a 30 °C difference in absolute rock surface temperature. The average percentage of the time dT/dt > 2 °C/min was ~ 8 ± 3%, > 4 °C/min was 1 ± 0.9%, and > 8 °C/min was 0.02 ± 0.03%. The maximum change over a 1-second interval was ~ 10 °C/min. When sampled to simulate data taken over intervals longer than 1 s, we found a reduction in time spent above the 2 °C/min temperature gradient threshold. For 1-minute samples, the time spent above any given threshold was about two orders of magnitude lower than the corresponding value for 1-second sampling. We suggest that a rough measure of efficacy of weathering as a function of frequency is the product of the percentage of time spent above a given threshold value multiplied by the damping depth for the corresponding frequency. This product has a broad maximum for periods between 3 and 10 s.     

低温胁迫下巴旦杏的形态生理变化及抗寒性研究

莎车1号、英吉沙2号和莎车18号是新疆喀什地区巴旦杏的主栽品种。通过测定低温胁迫下细胞膜透性、叶绿素荧光参数的变化,对巴旦杏品种的抗寒性进行了比较研究,并进行了冻害分级。结果表明,随处理温度降低,相对电导率逐渐增加,枝条细胞膜受损程度在加重。引起巴旦杏品种低温伤害的临界温度范围为-24～-27 ℃,3个品种枝条半致死温度（LT50）分别为-24.9 ℃、-29.3 ℃、-27.7 ℃,英吉沙2号的抗寒性较强。随处理温度降低,巴旦杏枝条基础荧光（Fo）值逐渐上升,至某一温度达到峰值后开始下降,光系统（PSⅡ）最大光化学效率（Fv/Fm）值呈明显下降趋势。-24～-27 ℃左右的低温会造成PSⅡ结构的损伤,且品种抗寒性越差,PSⅡ结构受损越明显。综合分析结果表明,供试的3个巴旦杏品种中, 英吉沙2号抗寒性较强,莎车18号次之,莎车1号抗寒性较弱。     

Effects of salinity, temperature and light on germination of invasive Prosopis juliflora (Sw.) D.C.

Effects of salinity, temperature, light and their interactions on the rate and final percentage of germination were evaluated for the invasive shrub Prosopis juliflora, grown under arid environmental conditions of the United Arab Emirates (UAE). Seeds that were not treated with NaCl germinated well in a wide range of temperatures and in both full light and complete dark. Seed germination decreased significantly with the increase in both NaCl concentration and temperature. Optimal germination percentage occurred at 25 °C. The inhibitory effect of high salinity on final germination percentage was greater at 40 °C than at 15 and 25 °C. Germination was completely inhibited in 400 mM NaCl at 40 °C and in 600 mM NaCl at 25 °C. However, germination rate was significantly greater at 40 °C than at 15 and 25 °C in lower salinities and the reverse was true at higher salinities. Germination in light was significantly greater than in the dark at lower salinity levels and high temperature.     

Li2O·nB2O3(n=4,5)-20%LiCl-H2O过饱溶液20℃结晶动力学研究

采用结晶动力学方法对Li2 O :B2 O3=1 :4和 1 :5在 2 0 %LiCl-H2 O中的过饱的溶液在 2 0℃时的结晶动力学过程进行了研究 ,两种不同Li2 O :B2 O3(摩尔比 )配比的过饱和溶液均析出LiB5O8·5H2 O一种固相 ,通过X -ray粉末衍射、IR光谱和热分析对结晶析出固相进行了表征。同时拟合给出了结晶动力学方程 ,并对结晶反应机理进行了探讨。     

宁夏0 ℃积温时空演变特征分析

利用宁夏全区25个气象台站日平均气温资料,统计分析≥0 ℃积温的时空演变及其开始、结束日期的变化特征;采用复Morlet小波分析方法和Mann-Kendall突变检测方法^〔1〕,揭示了宁夏不同区域≥0 ℃积温的周期及突变特征;并对其演变成因进行了初步探讨。结果表明：近50 a宁夏各区域≥0 ℃的积温均呈增大趋势,积温的持续时间明显延长;全区及引黄灌区0 ℃积温在1997年出现由少到多的突变,在周期上存在3～4 a和9 a左右的长周期;≥0 ℃积温期间引黄灌区极端最低气温<0 ℃的出现日数明显减少,由此而引起的冻害也相应减少。造成宁夏≥0 ℃积温增加、持续时间延长的主要原因是气候变暖前后大气环流出现了明显调整。变暖前,500 hPa高度场上中高纬度地区,特别是巴尔喀什湖附近易于形成低槽,有利于极地冷空气南下影响宁夏区域,造成气温偏低,≥0 ℃的积温偏小;气候变暖之后,500 hPa环流场发生了显著变化,中高纬度地区经向环流减弱,纬向环流增强,不利于槽区的形成和发展,极地南下冷空气活动次数减小,下游大范围区域内的气温升高,对应的0 ℃积温增加。     

温度对武夷山不同海拔土壤有机碳矿化的影响

采用碱吸收法测定武夷山不同海拔土壤分别在15℃、25℃、35℃下培养35d时土壤有机碳矿化速率及矿化量的变化．结果表明,土壤有机碳矿化速率随培养时间延长而逐渐降低,尤以培养3d～7d时下降最为明显．各海拔土壤累积矿化量均随培养温度升高而逐渐增加．培养35d时15℃和35℃下土壤累积矿化量随海拔升高而增加,但25℃下黄红壤的累积矿化量高于红壤和黄壤而低于山地草甸土．各培养温度下,土壤有机碳平均矿化速率均以山地草甸土最高,红壤最低．而对于各海拔土壤,不同温度下土壤有机碳平均矿化速率大小顺序为：15℃〈25℃〈35℃．培养3d时温度为15℃／25℃黄壤的Q10值显著高于其他海拔土壤（P〈0．05）,但培养35d时15℃／25℃下的土壤Q10值以黄红壤的最高．培养3d和35d时,25℃／35℃下不同海拔土壤Q10值差异均不显著（P〉0．05）．根据土壤平均矿化速率计算的Q10值在温度范围为15℃／25℃时以黄壤的最高,但25℃／35℃时红壤的Q10值最大．     

乔治王岛菲尔德斯半岛的地温特征和岩石热物理性质

1985—1986年度和1986—1987年度在菲尔德斯半岛对地面温度进行了测量,共获得129个数据,并对带回的样品进行了岩石热导率测量,共获得210个数据。本文在分析和归纳这些数据的基础上对菲尔德斯半岛的地温特征和岩石热物理性质进行了初步探讨。     

1981-2019年全球气温变化特征

1981—2019年全球气温变化特征是揭示全球气温变化的空间差异性以及实现全球共同应对气候变化的关键。本文基于7套再分析数据,采用气候变化速率及空间插值等分析方法,分析了1981—2019年全球气温变化时空特征及主要国家气温变化。结果表明：1981—2019年全球陆地气温以0.320 ℃/10a的速率呈极显著升高趋势,年平均气温增加了0.835 ℃;南、北半球陆地气温变化速率分别为0.147 ℃/10a、0.362 ℃/10a,均呈极显著增加趋势,分别增加了0.874 ℃、0.828 ℃。全球陆地80%面积上气温呈现显著增加趋势,年平均气温升高速率最大的区域位于80°N~90°N,其次是70°N~80°N、60°N~70°N,高纬大于中、低纬,格陵兰地区、乌克兰、俄罗斯等中高纬度国家或地区增温速率较快,尤以格陵兰地区增加速率最快,气温变化速率为0.654 ℃/10a;增温最慢的地区主要位于新西兰和赤道附近的南美洲、东南亚、非洲南部等地,气温变化速率不足0.15 ℃/10a。本文统计的146个国家中,年平均气温呈显著增加趋势的国家136个,占93%;气温无显著变化的国家10个,占6.849%。1981—2019年全球增温2.0 ℃以上、1.5 ℃以上、1.0 ℃以上的国家分别为4个、34个、68个,分别约占统计国家的2.740%、23.288%、46.575%。本文认为1998年以来全球并没有出现气温变暖停滞的现象。     

中国西北地区近50年夏季0 ℃层高度及气温时空变化特征

采用1960-2009 年112 个地面观测站和21 个探空站气象资料,分析中国西北地区近50 年夏季0 ℃层高度和平均气温变化趋势。结果表明：西北地区近50 年来夏季0 ℃层高度平均值为4675.7 m,在1987 年以前表现为下降趋势,平均每年下降5.6 m,而1987 年以后表现为显著的上升趋势,平均每年上升2.6 m;夏季平均气温与0 ℃层高度变化趋势大致相同,西北气候显著转型前后表现出相反的变化趋势,且1987 年以来夏季平均气温增加急剧,平均每10 年上升0.53 ℃。1960 年以来夏季0 ℃层高度在北京时间08 时该区大部分地区表现出显著的上升趋势,而在20时大部分地区表现出相反的变化趋势,1987 年以来在08 时和20 时大部分地区夏季0 ℃层高度都为上升趋势;50 年来大部分地区夏季平均气温都表现为上升趋势,乌鲁木齐和秦巴山地则表现为显著的下降趋势。西北不同气候区的夏季0 ℃层高度和平均气温变化也有一定的差异。西北地区夏季0 ℃层高度和平均气温具有较好相关性,东部季风区相关性最差,青藏高寒区最好。     

Lipid composition of phytoplankton from the Barents Sea and environmental influences on the distribution pattern of carbon among photosynthetic end products

The colonial algae Phaeocystis pouchetii and Dinobryon pellucidum dominated the phytoplankton crop at three stations in the Polar Front area of the Barents Sea.
Lipid extracted from the seawater containing the phytoplankton was dominated by neutral lipid classes, particularly triacylglycerols, and phospholipids were more abundant than galactolipids at all stations. Polyunsaturated fatty acids comprised between 15 and 26% of fatty acids of total lipid.
Of the carbon assimilated into lipid over 24 hours, 40% was located in the neutral lipid fraction. Phospholipids contained a smaller proportion of fixed carbon than galactolipids.
No defiinte relationships were observed between the distribution of fixed carbon in photosynthetic end products and the temperature or irradiance at which the phytoplankton was incubated. At a constant irradiance of 8.5 μmol m⁻²s⁻¹, the highest proportion of fixed carbon was recovered in protein at 4.5°C, but at −1.5°C most radioactivity was present in low molecular weight compounds. Regardless of incubation conditions, lipid always contained less than 30% of total assimilated carbon.     

南极乔治王岛菲尔德斯半岛土壤的化学风化作用

西南极南设得兰群岛乔治王岛菲尔德斯半岛土壤化学分析表明土壤中存在着一定的化学风化作用。相对于母质,土壤中的SiO_2、Al_2O_3、Fe_2O_3、FeO、MgO,GaO和Na_2O淋失,K_2O、H_2O~+、H_2O~-、TiO_2和P_2O_5富集。土壤中SiO_2/Al_2O_3的平均值为4.71,属弱风化壳类型。硅铝比反映出成土作用时间越长,土壤的风化程度越高,化学风化作用大于南极大陆边缘和内部。Al_2O_3在南极的淋失率大于SiO_2,使较老土壤剖面由下向上硅铝比增大。土壤的化学性质很大程度上取决于母质。南极强烈的冻融扰动作用破坏了土壤分层。土壤中存在着较强的水合作用。     

三种北极微藻对不同温度的适应性研究

为了研究北极微藻对不同环境温度的适应性,对分离自北极王湾地区的三种微藻-玛氏骨条藻(Skeletonema marinoi)、小球藻(Chlorella sp.)和衣藻(Chlamydomonas sp.)在不同温度条件下(0℃、4℃和8℃)的生长曲线和抗氧化酶系统进行了测定.结果表明微藻对温度的适应性因种而异:玛氏骨条藻在4℃具有最快生长速率,最高的超氧化物歧化酶(SOD)和过氧化氢酶(CAT)酶活性,及最低的丙二醛(MDA)含量;小球藻的最快生长速率及酶活均在8℃时,MDA含量最低值出现在0℃;衣藻生长情况相差不大,MDA含量的最低值出现在8℃.抗氧化酶系统变化规律也因藻种及温度而异--在0℃与4℃变化时,玛氏骨条藻抗氧化酶系统的三个指标的差异极显著(P<0.01);在0℃与8℃变化时,玛氏骨条藻与小球藻的SOD活性的差异显著(P<0.05),其余两个指标差异不显著;衣藻在三个温度下的三个指标差异均不显著.总体而言,三种藻对三个温度的适应性均较好:其MDA的含量均较低,三种藻各自具有独特的生理代谢机制来适应不同的温度环境.     

Seed germination of five Helianthemum species: Effect of temperature and presowing treatments

The germination behaviour of five Helianthemum species (H. almeriense, H. appeninum, H. cinereum, H. hirtum, H. squamatum) has been studied under controlled conditions. Constant 15, 20 or 25 °C and alternating 25/15 °C temperature regimes and 16/8 h light/dark photoperiod conditions were used. Presowing treatments applied were manual scarification, boiling water, hot water, dry heat and sulphuric acid. Germination values recorded were final germination percentage and germination rate expressed as days to reach 50% of the final germination percentage (T₅₀). Incubation temperature had no significant effect on final germination percentage for untreated seeds of the five Helianthemum species. However, variation due to temperature was significant for scarified seeds, with the lowest germination percentage attained at 25 °C. In all Helianthemum species studied, the highest germination percentages were obtained by manual scarification of seeds. Germination rate of scarified seeds decreased as germination temperature increased. The different presowing treatments investigated allowed some germination in some species, but none were any better than manual scarification. The high germination among most species studied, following mechanical rupture of the seed coat, shows that the mechanism of dormancy lies in the seed coat. The physical dormancy caused by impermeable seed-coat appears to be the main reason of poor germination of untreated seeds of Helianthemum species studied.     

南极长城站海湾夏季底层海水温度变化特征

本文介绍了在南极长城站海湾对底层海水温度所进行的长期自动观测,并用已获得的1987年1月至4月90天的连续观测记录,首次对夏季底层海水温度的变化特征进行了分析。     

异向性岩芯冰的反复压缩-退火实验研究

在蠕变试验机上完成了三组(6块样品)单轴反复压缩-退火实验,试验机载荷精度为1％,实验温度为-2±0.2℃。实验样品取自南极洛多姆冰帽BHQ钻孔的冰岩芯。三组试样的受压方向分别与冰岩芯的轴向平行、成45°角和垂直。试验前试样的C轴组构为单极大型或大致为单极大型,晶粒大小不一。用0.8MPa的初始轴向应力对样品进行压缩,达到10％应变后卸载退火72小时。每组实验如此反复进行6次。结果表明,在较高的温度和大载荷下,样品经反复压缩-退火,其初始组构特征消失,代之以小环状组构和细的、等粒的晶体,某些情况下发展为多极大型组构。结构和组构分析表明,新组构的形成机制主要是再结晶作用。随着压缩-退火反复进行,六块样品在组构上的差异减少,它们的流变特性趋于一致,晶粒尺寸逐渐减少而趋于一个稳定值。